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Determining differential effects of Interleukin-2 on innate and adaptive immune cells in lymphoid organs and the gastrointestinal Tract

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Date Issued:
2019
Abstract/Description:
Interleukin-2 (IL-2) is a pleiotropic cytokine demonstrated to be effective in treating cancer. However, the clinical use of IL-2 can be associated with severe side effects including gastrointestinal toxicity (GT). Similar GT symptoms are observed in inflammatory diseases such as CD (CD). Interestingly mounting evidence indicates a role for IL-2 in CD, but the underlying mechanisms are unknown. Indeed, studies on the in-vivo activities of IL-2 have mostly focused on secondary lymphoid organs and immune cells associated with them. Very few studies have addressed how IL-2 signals impact populations of immune cells in the gut. Here, we aim to identify and compare the effects of systemic IL-2 administration on six major leukocyte population and their subsets in mice using multicolor flow cytometry. While we confirmed previously observed changes in specific immune cell populations in the spleen, very few changes were seen in the gut and gut associated lymphoid tissues. Unexpectedly, a sharp decline was seen in B cells, most notably in Peyer's Patches, in mice treated with IL-2. Our data furthermore indicates that B cells in IL-2 treated mice undergo enhanced apoptosis in Peyer's Patches. Some studies suggest that changes in B cells may contribute to development of CD. Thus, this study may aid in defining ways in which IL-2 can contribute to disease etiology, and lead to novel treatments for CD.
Title: Determining differential effects of Interleukin-2 on innate and adaptive immune cells in lymphoid organs and the gastrointestinal Tract.
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Name(s): Singh, Ayushi, Author
McKinstry, Karl, Committee Chair
Naser, Saleh, Committee CoChair
Copik, Alicja, Committee Member
University of Central Florida, Degree Grantor
Type of Resource: text
Date Issued: 2019
Publisher: University of Central Florida
Language(s): English
Abstract/Description: Interleukin-2 (IL-2) is a pleiotropic cytokine demonstrated to be effective in treating cancer. However, the clinical use of IL-2 can be associated with severe side effects including gastrointestinal toxicity (GT). Similar GT symptoms are observed in inflammatory diseases such as CD (CD). Interestingly mounting evidence indicates a role for IL-2 in CD, but the underlying mechanisms are unknown. Indeed, studies on the in-vivo activities of IL-2 have mostly focused on secondary lymphoid organs and immune cells associated with them. Very few studies have addressed how IL-2 signals impact populations of immune cells in the gut. Here, we aim to identify and compare the effects of systemic IL-2 administration on six major leukocyte population and their subsets in mice using multicolor flow cytometry. While we confirmed previously observed changes in specific immune cell populations in the spleen, very few changes were seen in the gut and gut associated lymphoid tissues. Unexpectedly, a sharp decline was seen in B cells, most notably in Peyer's Patches, in mice treated with IL-2. Our data furthermore indicates that B cells in IL-2 treated mice undergo enhanced apoptosis in Peyer's Patches. Some studies suggest that changes in B cells may contribute to development of CD. Thus, this study may aid in defining ways in which IL-2 can contribute to disease etiology, and lead to novel treatments for CD.
Identifier: CFE0007865 (IID), ucf:52777 (fedora)
Note(s): 2019-12-01
M.S.
Medicine,
Masters
This record was generated from author submitted information.
Subject(s): IL-2 -- GUT -- GALT (gut associated lymphoid tissues) -- S4B6 IL-2C -- Lungs -- Spleen -- PP's -- MLN -- Lamina Propria -- B cells -- Tcells -- Crohn's Disease -- Gastrointestinal Toxicity
Persistent Link to This Record: http://purl.flvc.org/ucf/fd/CFE0007865
Restrictions on Access: campus 2020-12-15
Host Institution: UCF

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