Current Search:  Ovarian Cancer (x)

View All Items

  • CSV Spreadsheet
(1 - 4 of 4)
TO EVALUATE THE FUNCTION OF THE OXYTOCIN RECEPTOR IN THE CONTEXT OF OVARIAN CANCER CELL MICROENVIRONMENT TO DETERMINE IF OXYTOCIN CAN INDUCE AN ANTI-INFLAMMATORY RESPONSE
Title
TO EVALUATE THE FUNCTION OF THE OXYTOCIN RECEPTOR IN THE CONTEXT OF OVARIAN CANCER CELL MICROENVIRONMENT TO DETERMINE IF OXYTOCIN CAN INDUCE AN ANTI-INFLAMMATORY RESPONSE.
Creator
Schachner, Benjamin I, Samsam, Mohtashem, University of Central Florida
Abstract / Description
The treatment of most cancers can still be considered inadequate despite the steady progress being made. A prime example of this issue is with epithelial ovarian cancers; this disease presents a significant issue, with a 5-year survival rate of 46% and a survival rate of 28% in patients that develop metastatic disease. Since ovarian cancer has such a high mortality rate, effective treatment modalities are necessary to prolong the quality of life after diagnosis. Psychosocial stress is related...
Show moreThe treatment of most cancers can still be considered inadequate despite the steady progress being made. A prime example of this issue is with epithelial ovarian cancers; this disease presents a significant issue, with a 5-year survival rate of 46% and a survival rate of 28% in patients that develop metastatic disease. Since ovarian cancer has such a high mortality rate, effective treatment modalities are necessary to prolong the quality of life after diagnosis. Psychosocial stress is related to the progression, proliferation, and migration in cancer patients, but the mechanisms of this relationship are not fully understood. The present in vitro study investigated the ability of oxytocin, a neuropeptide associated with social support, to attenuate the stress response. Catecholamines, a subclass of stress hormones, were used to simulate the stress induced inflammation process in ovarian cancer cells. To evaluate oxytocin's capacity to attenuate the stress response, the ovarian cancer cell lines SKOV3, HEYA8, OVCAR8, and OV432 were separately treated with the presence or absence of catecholamines with the addition of oxytocin. Protein expression of the oxytocin receptor was investigated using a western blot protocol. Oxytocin receptor, oxytocin, and IL-6 mRNA expression was evaluated by quantitative PCR. Treatment with Oxytocin attenuated the inflammatory response resulting from catecholamine treatment. The oxytocin receptor gene and protein were present in each cell line, suggesting that oxytocin has an anti-inflammatory role in the tumor microenvironment in ovarian cancer patients. These results provide a mechanism by which social support, working through the release of oxytocin, promotes an anti-inflammatory process in ovarian cancer patients. This study may shed light into new pharmacological approaches for the treatment of ovarian cancer.
Show less
Date Issued
2017
Identifier
CFH2000344, ucf:45809
Format
Document (PDF)
PURL
http://purl.flvc.org/ucf/fd/CFH2000344
ONLINE SUPPORT GROUP FOR CHINESE WOMEN WITH OVARIAN OR CERVICAL CANCER
Title
ONLINE SUPPORT GROUP FOR CHINESE WOMEN WITH OVARIAN OR CERVICAL CANCER.
Creator
Xing, Yuan, Loerzel, Victoria, University of Central Florida
Abstract / Description
Women with gynecological (GYN) cancer experience a wide spectrum of physical, emotional and social effects after diagnosis and treatment of their cancer. The insufficient availability of local support groups, limited transportation for the rural Chinese population and a shortage of oncologists make it difficult to have face-to-face support groups for Chinese GYN cancer patients. However, the wide access of Internet has provided an opportunity for people to have online support groups. The...
Show moreWomen with gynecological (GYN) cancer experience a wide spectrum of physical, emotional and social effects after diagnosis and treatment of their cancer. The insufficient availability of local support groups, limited transportation for the rural Chinese population and a shortage of oncologists make it difficult to have face-to-face support groups for Chinese GYN cancer patients. However, the wide access of Internet has provided an opportunity for people to have online support groups. The purpose of this study was to observe and describe the types of support given to and by Chinese GYN cancer survivors in a QQ chat group. This was a qualitative study that used the directed content analysis approach. A QQ group was observed for two weeks between March 10th (12:01am) and March 24st (11:59pm) 2018 Beijing time. Observed online posts were copied and pasted into a WORD ™ document for analysis. There were 4 themes observed: sharing experience, information exchange, emotional support and Guardian Against Cancer group member benefits. The results of the study suggested that women supported each other but little evidence-based support was observed. Healthcare providers should be monitoring and engaging in conversations with group members. Well-planed and organized information sessions should also be beneficial for members. Further research on understanding members' needs on online support groups and the effectiveness of intervention should be conducted.
Show less
Date Issued
2018
Identifier
CFH2000382, ucf:45808
Format
Document (PDF)
PURL
http://purl.flvc.org/ucf/fd/CFH2000382
A novel link between Akt1 and Twist1 in ovarian tumor cell motility and invasiveness
Title
A novel link between Akt1 and Twist1 in ovarian tumor cell motility and invasiveness.
Creator
Shah, Nirav, Altomare, Deborah, Zhao, Jihe, Khaled, Annette, University of Central Florida
Abstract / Description
Ovarian cancer results in more deaths per year than any other cancer of the female reproductive system. The low survival rate is partly due to the lack of early detection and the susceptibility to relapse. The AKT serine threonine kinase plays a pivotal role in hallmark cellular processes for the progression of ovarian cancer, including tumor cell growth and migration. Therapeutic targeting of pan-AKT has been problematic, in part due to feedback mechanisms and crosstalk with other pathways....
Show moreOvarian cancer results in more deaths per year than any other cancer of the female reproductive system. The low survival rate is partly due to the lack of early detection and the susceptibility to relapse. The AKT serine threonine kinase plays a pivotal role in hallmark cellular processes for the progression of ovarian cancer, including tumor cell growth and migration. Therapeutic targeting of pan-AKT has been problematic, in part due to feedback mechanisms and crosstalk with other pathways. The hypothesis for this study is that AKT 1, -2 and -3 isoforms may have different roles and regulate cell processes in uniquely varied ways. A transgenic mouse model that expresses the SV40 T-antigen viral oncogene and is known to have dramatically increased susceptibility to ovarian cancer was utilized, and it had genetic inactivation of either AKT1 or AKT2 through targeted deletion of the individual genes because these isoforms have been implicated in this cancer. Primary ovarian tumor cell cultures were established and found to exhibit different morphology, proliferation and migration that may indicate a different role for the AKT1 and AKT2 isoforms in these contexts. Ovarian tumor cells with absence of AKT1 predominantly exhibited reduced cell migration when compared to cells with retention of AKT1 and absence of AKT2. Since AKT is known to be important for epithelial-mesenchymal transition (EMT), a process potentially associated with tumor cell metastasis, the expression of transcription factors implicated in EMT was assessed by real-time array analysis in ovarian tumor cells knocked-out for either AKT1 or AKT2. Twist1, one of the major players in EMT, was not detectable in the cells missing the AKT1 isoform. Results indicate an association of Twist1 with AKT1 in EMT and migration of ovarian tumors cells. This finding is significant because AKT2 has been implicated as the major player of cell migration in human breast cancer cells. Collectively, these findings support a tissue specific role of the AKT isoforms, and may provide insights regarding the most useful cell context in order to target components of the AKT signaling pathway indirectly affecting EMT in order to prevent tumor progression in patients with ovarian and perhaps other types of cancers.
Show less
Date Issued
2012
Identifier
CFE0004630, ucf:49916
Format
Document (PDF)
PURL
http://purl.flvc.org/ucf/fd/CFE0004630
Development of Cytotoxic Natural Killer Cells for Ovarian Cancer Treatment
Title
Development of Cytotoxic Natural Killer Cells for Ovarian Cancer Treatment.
Creator
Pandey, Veethika, Altomare, Deborah, Zhao, Jihe, Khaled, Annette, Estevez, Alvaro, University of Central Florida
Abstract / Description
Ovarian cancer is a leading cause of gynecological malignancy. Cytoreductive surgery and frontline platinum/taxane-based chemotherapy provides good initial efficacy in the treatment, but poor long-term patient survival. This is mainly caused by tumor relapse due to intraperitoneal spreading and ineffective alternate therapies to treat these resistant tumors. The challenge in the field is to develop strategies that would prove effective in these patients and extend overall survival.Over the...
Show moreOvarian cancer is a leading cause of gynecological malignancy. Cytoreductive surgery and frontline platinum/taxane-based chemotherapy provides good initial efficacy in the treatment, but poor long-term patient survival. This is mainly caused by tumor relapse due to intraperitoneal spreading and ineffective alternate therapies to treat these resistant tumors. The challenge in the field is to develop strategies that would prove effective in these patients and extend overall survival.Over the years, various treatments have been developed for the treatment of cancer amongst which, adoptive cell immunotherapy has shown promising results. But despite the efficacy seen in the clinic, there are concerns with the complexity of treatment and associated side effects. Therefore, there is still a need for better understanding of how different components of the immune system react to the presence of tumor. In this study, healthy human peripheral blood mononuclear cells (PBMCs) were used to examine the immune response in a mouse model with residual human ovarian tumor, where natural killer (NK) cells were found to be the effector cells that elicited an anti-tumor response. Presence of tumor was found to stimulate NK cell expansion and cytotoxicity in mice treated intraperitoneally (IP) with PBMCs+Interleukin-2 (IL- 2). Intravenous (IV) adoptive transfer of isolated NK cells has been attempted in ovarian cancer patients before, but showed lack of persistence in patients resulting in lack of anti-tumor efficacy. Experiments in this study highlight the significance of NK cell-cytotoxic response to tumor, which may be attributed to interacting immune cell types in the PBMC population (when treated IP), as opposed to clinically used isolated NK cells showing lack of anti-tumor efficacy in ovarian cancer patients (when treated IV).iiiNK cell immunotherapy is mainly limited by insufficient numbers generated for adoptive transfer, limited in vivo life span after adoptive transfer, lack of cytotoxicity and some logistical concerns that impede its widespread implementation. Therefore there is a need to develop methods of NK cell expansion that provide stimulation similar to other immune cell types in the PBMC population. The second part of this study utilizes a method of in vivo NK cell expansion using a particle-based approach in which plasma membranes of K562-MB21-41BBL cells (K562 cells expressing membrane-bound IL-21 and 41BB ligand) are used for specific NK cell expansion from PBMCs. NK cells expanded with this method were cytotoxic, showed in vivo persistence and biodistribution in different organs.Collectively, these studies show that NK cells are a major innate immune component that can recognize and kill the tumor. Their cytotoxic ability, using particle-based stimulation, can be enhanced for a second-line treatment of relapsed tumors such as in ovarian cancer as well as other cancer types.
Show less
Date Issued
2015
Identifier
CFE0006369, ucf:51531
Format
Document (PDF)
PURL
http://purl.flvc.org/ucf/fd/CFE0006369