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- Title
- SEXUALITY AND COURTSHIPS OF 19TH CENTURY VIENNA VS 21ST CENTURY AMERICA THROUGH THE ROLES OF ALFRED AND SAM IN ROMANCE, ROMANCE.
- Creator
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Haynie, James, Weaver, Earl, University of Central Florida
- Abstract / Description
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For my thesis, I plan to research the sexuality and the process of courtship, and the differences between 19th century Vienna and modern America, specifically as to how it relates to my characters in Romance, Romance. My interest in the topic springs directly from the script. What I mean is, my initial idea of sexuality of the 19th century is very Victorian and straight-laced, where as I think of modern day as very sexually liberated. However, in the script, the first act, set in Vienna, is...
Show moreFor my thesis, I plan to research the sexuality and the process of courtship, and the differences between 19th century Vienna and modern America, specifically as to how it relates to my characters in Romance, Romance. My interest in the topic springs directly from the script. What I mean is, my initial idea of sexuality of the 19th century is very Victorian and straight-laced, where as I think of modern day as very sexually liberated. However, in the script, the first act, set in Vienna, is much more sexually explicit and active. In fact, the second act is all building up to an affair that does not even occur. Working as these characters, I believe that a historical view of what sexuality was actually like outside of Victorian England will lead me to a much better understanding of my character, and his particular past and desires for both sexual partners and potential mates. I also believe this research will assist me in constructing the modern character of Sam who can all at once love his wife, desperately want an affair, and cower away from said affair when the opportunity finally arises. Another interesting impact this research will create for my work is the juxtaposition of these two time periods and their individual codes of etiquette for behavior in this play. While the play is divided into two separate one-acts, they are presented together as one collective work, so the combination of each piece clearly leads to comparisons and further understanding of the other play. I believe this research will not only enlighten me about these two eras but also enlighten my performance, and assist me in effectively carrying out this complex pair of roles with a richer character developed. It also will provide me with a better understanding of the juxtaposition of these two plays, why they are paired together in the way they have been, and how this juxtaposition is effective in this particular work of musical theatre.
Show less - Date Issued
- 2008
- Identifier
- CFE0002380, ucf:47820
- Format
- Document (PDF)
- PURL
- http://purl.flvc.org/ucf/fd/CFE0002380
- Title
- PATTERNED CELL CULTURES FOR HIGH THROUGHPUT STUDIES OF CELL ELECTROPHYSIOLOGY AND DRUG SCREENING APPLICATIONS.
- Creator
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Natarajan, Anupama, Hickman, James, University of Central Florida
- Abstract / Description
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Over the last decade, the field of tissue and bio-engineering has seen an increase in the development of in vitro high-throughput hybrid systems that can be used to understand cell function and behavior at the cellular and tissue levels. These tools would have a wide array of applications including for implants, drug discovery, and toxicology, as well as for studying cell developmental behavior and as disease models. Currently, there are a limited number of efficient, functional drug...
Show moreOver the last decade, the field of tissue and bio-engineering has seen an increase in the development of in vitro high-throughput hybrid systems that can be used to understand cell function and behavior at the cellular and tissue levels. These tools would have a wide array of applications including for implants, drug discovery, and toxicology, as well as for studying cell developmental behavior and as disease models. Currently, there are a limited number of efficient, functional drug screening assays in the pharmacology industry and studies of cell-surface interactions are complicated and invasive. Most cell physiology studies are performed using conventional patch-clamp techniques or random networks cultured on silicon devices such as Microelectrode Arrays (MEAs) and Field Effect transistors (FETs). The objective of this study was to develop high-throughput in vitro platforms that could be used to analyze cell function and their response to various stimuli. Our hypothesis was that by utilizing surface modification to provide external guidance cues for various cell types and by controlling the cell environment in terms of culture conditions, we could develop an in vitro hybrid platform for sensing and testing applications. Such a system would not only give information regarding the surface effects on the growth and behavior of cells for implant development applications, but also allow for the study of vital cell physiology parameters like conduction velocity in cardiomyocytes and synaptic plasticity in neuronal networks. This study outlines the development of these in vitro high throughput systems that have varied applications ranging from tissue engineering to drug development. We have developed a simple and relatively high-throughput method in order to test the physiological effects of varying chemical environments on rat embryonic cardiac myocytes in order to model the degradation effects of polymer scaffolds. Our results, using our simple test system, are in agreement with earlier observations that utilized a complex 3D biodegradable scaffold. Thus, surface functionalization with self-assembled monolayers combined with histological/physiological testing could be a relatively high throughput method for biocompatibility studies and for the optimization of the material/tissue interface in tissue engineering. Traditional multielectrode extracellular recording methods were combined with surface patterning of cardiac myocyte monolayers to enhance the information content of the method; for example, to enable the measurement of conduction velocity, refractory period after action potentials or to create a functional reentry model. Two drugs, 1-Heptanol, a gap junction blocker, and Sparfloxacin, a fluoroquinone antibiotic, were tested in this system. 1-Heptanol administration resulted in a marked reduction in conduction velocity, whereas Sparfloxacin caused rapid, irregular and unsynchronized activity, indicating fibrillation. As shown in these experiments, the patterning of cardiac myocyte monolayers increased the information content of traditional multielectrode measurements. Patterning techniques with self-assembled monolayers on microelectrode arrays were also used to study the physiological properties of hippocampal networks with functional uni-directional connectivity, developed to study the mono-synaptic connections found in the dentate gyrus. Results indicate that changes in synaptic connectivity and strength were chemically induced in these patterned hippocampal networks. This method is currently being used for studying long term potentiation at the cellular level. For this purpose, two cell patterns were optimized for cell migration onto the pattern as demonstrated by time lapse studies, and for supporting the best pattern formation and cell survival on these networks. The networks formed mature interconnected spiking neurons. In conclusion, this study demonstrates the development and testing of in vitro high-throughput systems that have applications in drug development, understanding disease models and tissue engineering. It can be further developed for use with human cells to have a more predictive value than existing complex, expensive and time consuming methods.
Show less - Date Issued
- 2010
- Identifier
- CFE0003384, ucf:48437
- Format
- Document (PDF)
- PURL
- http://purl.flvc.org/ucf/fd/CFE0003384
