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DEVELOPMENT OF LUMINESCENT RUTHENIUM COMPLEXES FOR IN-VITRO FLUORESCENCE IMAGING OF ANGIOGENESIS WITH THE RGD PEPTIDE.
| Title: | DEVELOPMENT OF LUMINESCENT RUTHENIUM COMPLEXES FOR IN-VITRO FLUORESCENCE IMAGING OF ANGIOGENESIS WITH THE RGD PEPTIDE. |
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| Name(s): |
Victoria, Rosmery, Author Hinkle, Charles R., Committee Chair University of Central Florida, Degree Grantor |
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| Type of Resource: | text | |
| Date Issued: | 2012 | |
| Publisher: | University of Central Florida | |
| Language(s): | English | |
| Abstract/Description: | Herein we report the synthesis of an RGD-ruthenium bipyridine 2+ complex aimed at the detection of angiogenesis. Angiogenesis plays a critical role in many pathophysiological processes, such as tumor growth. The alpha v-integrins (alpha v beta 3, alpha v beta 5) are currently used as molecular targeting sites for anti-angiogenic therapies. The 2+ complex is an organometallic luminescent probe, which enables noninvasive, in vitro imaging of alpha v beta 3 expression. Peptides containing the arginine-glycine-aspartic acid (RGD) sequence have been shown to bind strongly to the alpha v beta 3 integrin. The RuBpy probes are soluble in water, display long lifetimes, and are photochemically stable. These properties enable the Ru(tris-bpy) complexes to be useful in numerous applications in biophysical and cell biology. The 2+ complex was synthesized by combining the succinimidyl ester on the RuBpy complex with the lysine of the c(RGDfK) peptide. The results of the one-photon fluorescence bioimaging showed selective binding of the cyclic RGD to alpha v beta 3 integrin, which supports previous literature. The high luminescence intensity, long lifetimes, and low cell toxicity levels of dye 2+, illustrates the potential usage of this probe for future biological applications. | |
| Identifier: | CFH0004234 (IID), ucf:44898 (fedora) | |
| Note(s): |
2012-05-01 B.S. Sciences, Dept. of Biology Bachelors This record was generated from author submitted information. |
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| Subject(s): |
Ruthenium alpha v beta 3 integrin RGD Peptide organometalic probes long lifetimes angiogenesis early tumor detection Ruthenium bipyridine RGD-ruthenium bipyridine complex cell bio imaging cyclic RGD Luminescent fluorescence |
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| Persistent Link to This Record: | http://purl.flvc.org/ucf/fd/CFH0004234 | |
| Restrictions on Access: | public 2012-04-01 | |
| Host Institution: | UCF |
