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DEVELOPMENT OF LUMINESCENT RUTHENIUM COMPLEXES FOR IN-VITRO FLUORESCENCE IMAGING OF ANGIOGENESIS WITH THE RGD PEPTIDE.

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Date Issued:
2012
Abstract/Description:
Herein we report the synthesis of an RGD-ruthenium bipyridine 2+ complex aimed at the detection of angiogenesis. Angiogenesis plays a critical role in many pathophysiological processes, such as tumor growth. The alpha v-integrins (alpha v beta 3, alpha v beta 5) are currently used as molecular targeting sites for anti-angiogenic therapies. The 2+ complex is an organometallic luminescent probe, which enables noninvasive, in vitro imaging of alpha v beta 3 expression. Peptides containing the arginine-glycine-aspartic acid (RGD) sequence have been shown to bind strongly to the alpha v beta 3 integrin. The RuBpy probes are soluble in water, display long lifetimes, and are photochemically stable. These properties enable the Ru(tris-bpy) complexes to be useful in numerous applications in biophysical and cell biology. The 2+ complex was synthesized by combining the succinimidyl ester on the RuBpy complex with the lysine of the c(RGDfK) peptide. The results of the one-photon fluorescence bioimaging showed selective binding of the cyclic RGD to alpha v beta 3 integrin, which supports previous literature. The high luminescence intensity, long lifetimes, and low cell toxicity levels of dye 2+, illustrates the potential usage of this probe for future biological applications.
Title: DEVELOPMENT OF LUMINESCENT RUTHENIUM COMPLEXES FOR IN-VITRO FLUORESCENCE IMAGING OF ANGIOGENESIS WITH THE RGD PEPTIDE.
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Name(s): Victoria, Rosmery, Author
Hinkle, Charles R., Committee Chair
University of Central Florida, Degree Grantor
Type of Resource: text
Date Issued: 2012
Publisher: University of Central Florida
Language(s): English
Abstract/Description: Herein we report the synthesis of an RGD-ruthenium bipyridine 2+ complex aimed at the detection of angiogenesis. Angiogenesis plays a critical role in many pathophysiological processes, such as tumor growth. The alpha v-integrins (alpha v beta 3, alpha v beta 5) are currently used as molecular targeting sites for anti-angiogenic therapies. The 2+ complex is an organometallic luminescent probe, which enables noninvasive, in vitro imaging of alpha v beta 3 expression. Peptides containing the arginine-glycine-aspartic acid (RGD) sequence have been shown to bind strongly to the alpha v beta 3 integrin. The RuBpy probes are soluble in water, display long lifetimes, and are photochemically stable. These properties enable the Ru(tris-bpy) complexes to be useful in numerous applications in biophysical and cell biology. The 2+ complex was synthesized by combining the succinimidyl ester on the RuBpy complex with the lysine of the c(RGDfK) peptide. The results of the one-photon fluorescence bioimaging showed selective binding of the cyclic RGD to alpha v beta 3 integrin, which supports previous literature. The high luminescence intensity, long lifetimes, and low cell toxicity levels of dye 2+, illustrates the potential usage of this probe for future biological applications.
Identifier: CFH0004234 (IID), ucf:44898 (fedora)
Note(s): 2012-05-01
B.S.
Sciences, Dept. of Biology
Bachelors
This record was generated from author submitted information.
Subject(s): Ruthenium
alpha v beta 3 integrin
RGD Peptide
organometalic probes
long lifetimes
angiogenesis
early tumor detection
Ruthenium bipyridine
RGD-ruthenium bipyridine complex
cell bio imaging
cyclic RGD
Luminescent
fluorescence
Persistent Link to This Record: http://purl.flvc.org/ucf/fd/CFH0004234
Restrictions on Access: public 2012-04-01
Host Institution: UCF

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