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SQUARAINE DYES FOR TWO-PHOTON FLUORESCENCE BIOIMAGING APPLICATIONS

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Date Issued:
2013
Abstract/Description:
Near-infrared emitting squaraine dyes are promising candidates for bioimaging applications. Two-photon fluorescence microscopy (2PFM) imaging is a powerful tool being used for studying biological function since it produces 3D images with minimal damage to cells and lower fluorophore photobleaching. The fluorescence wavelength of squaraine dyes normally falls in the near infrared region, providing deeper penetration through biological samples such as thick tissue sections. Squaraine dyes that could work for imaging cells and tissues for 2PFM imaging were synthesized and underwent comprehensive photophysical characterization, such as UV-Vis absorption, fluorescence, and anisotropy. The squaraine dyes were tested for cell toxicity to determine the concentration at which the cells should be incubated with the dye for 2PFM. In addition, the squaraine dyes were incubated with cancer cells to evaluate their utility in the bioimaging process. The squaraine dye that is not soluble in water can be incorporated in silica nanoparticles or micelles to facilitate dispersal in water for evaluation of its use as a probe. The prospective squaraine dyes can be used in cells and tissues for imaging that can then be analyzed to ascertain its use as a probe for biomedical applications, such as early cancer detection.
Title: SQUARAINE DYES FOR TWO-PHOTON FLUORESCENCE BIOIMAGING APPLICATIONS.
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Name(s): Colon Gomez, Maria, Author
Belfield, Kevin, Committee Chair
University of Central Florida, Degree Grantor
Type of Resource: text
Date Issued: 2013
Publisher: University of Central Florida
Language(s): English
Abstract/Description: Near-infrared emitting squaraine dyes are promising candidates for bioimaging applications. Two-photon fluorescence microscopy (2PFM) imaging is a powerful tool being used for studying biological function since it produces 3D images with minimal damage to cells and lower fluorophore photobleaching. The fluorescence wavelength of squaraine dyes normally falls in the near infrared region, providing deeper penetration through biological samples such as thick tissue sections. Squaraine dyes that could work for imaging cells and tissues for 2PFM imaging were synthesized and underwent comprehensive photophysical characterization, such as UV-Vis absorption, fluorescence, and anisotropy. The squaraine dyes were tested for cell toxicity to determine the concentration at which the cells should be incubated with the dye for 2PFM. In addition, the squaraine dyes were incubated with cancer cells to evaluate their utility in the bioimaging process. The squaraine dye that is not soluble in water can be incorporated in silica nanoparticles or micelles to facilitate dispersal in water for evaluation of its use as a probe. The prospective squaraine dyes can be used in cells and tissues for imaging that can then be analyzed to ascertain its use as a probe for biomedical applications, such as early cancer detection.
Identifier: CFH0004338 (IID), ucf:45020 (fedora)
Note(s): 2013-05-01
B.S.
Sciences, Dept. of Chemistry
Bachelors
This record was generated from author submitted information.
Subject(s): squaraines dyes
bioimaging
two-photon absorption
two-photon fluorescence microscopy
cancer detection
Persistent Link to This Record: http://purl.flvc.org/ucf/fd/CFH0004338
Restrictions on Access: public 2013-04-01
Host Institution: UCF

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