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MELATONIN AND NEUROGENESIS: A COMPARATIVE STUDY OF THE EFFICACY OF MELATONIN, ITS PRECURSORS, AND L-DOPA ON NEURAL STEM CELL METABOLISM IN HUMAN ADULT NEUROSPHERES
| Title: | MELATONIN AND NEUROGENESIS: A COMPARATIVE STUDY OF THE EFFICACY OF MELATONIN, ITS PRECURSORS, AND L-DOPA ON NEURAL STEM CELL METABOLISM IN HUMAN ADULT NEUROSPHERES. |
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| Name(s): |
Heriba, Omar, Author Sugaya, Kiminobu, Committee Chair University of Central Florida, Degree Grantor |
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| Type of Resource: | text | |
| Date Issued: | 2014 | |
| Publisher: | University of Central Florida | |
| Language(s): | English | |
| Abstract/Description: | Human neurosphere stem cells offer promising potential for the treatment of neurodegenerative diseases. Their well characterized multi-potency of differentiating into neurons, astrocytes, and oligodendrocytes when exposed to the optimum exogenous growth factors make them an exciting area of study (38). Finding novel endogenous methods of modulating stem cell metabolism will allow for the safer treatment of various brain disorders (34). In this experiment, melatonin, N-acetylserotonin, L-tryptophan, and L-DOPA are added in three different concentrations to neurospheres suspended in HNSC/GBM media with less than optimal concentrations of exogenous epidermal growth factor (EGF) and fibroblast growth factor (FGF). The alamarBlue assay (resazurin) was chosen as the most suitable assay for measuring neurosphere metabolism. Metabolic neural stem cells would cause the greatest reduction of the oxidized alamarBlue reagent (resazurin-resorufin), which was detected by a fluorescent plate reader (39-41). The percent reduction in alamarBlue was calculated for all four molecules at three different concentrations and compared to controls without any molecule. Our results illustrate that there was no statistically significant difference at p<0.05 between the biological molecules and the control group except for two exceptions (labeled with asterisks on figures 3 and 5) L-DOPA at a 40 micromolar concentration after 4 hours of incubation and melatonin at a 40 micromolar concentration after 52 hours of incubation. | |
| Identifier: | CFH0004688 (IID), ucf:45239 (fedora) | |
| Note(s): |
2014-12-01 B.S. Medicine, Burnett School of Biomedical Sciences Bachelors This record was generated from author submitted information. |
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| Subject(s): |
neurospheres neural stem cells melatonin serotonin tryptophan L-DOPA alamarBlue |
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| Persistent Link to This Record: | http://purl.flvc.org/ucf/fd/CFH0004688 | |
| Restrictions on Access: | public | |
| Host Institution: | UCF |
