You are here
IN VITRO SELECTION OF DNA APTAMERS AGAINST PROSTATE CANCER PEPTIDE BIOMARKERS
| Title: | IN VITRO SELECTION OF DNA APTAMERS AGAINST PROSTATE CANCER PEPTIDE BIOMARKERS. |
 61 views 25 downloads |
|---|---|---|
| Name(s): |
Kuguoglu, Elif, Author Kolpashchikov, Dmitry, Committee Chair University of Central Florida, Degree Grantor |
|
| Type of Resource: | text | |
| Date Issued: | 2014 | |
| Publisher: | University of Central Florida | |
| Language(s): | English | |
| Abstract/Description: | This project is aimed toward finding DNA aptamers against prostate cancer peptide antigens. DNA aptamers can function to find and indicate the presence of certain molecules in a specimen. These aptamers will be obtained through the process of evolutionary selection, a specific process called SELEX which stands for Systemic Evolution of Ligands by Experimental Enrichment. By conducting several rounds of SELEX, a DNA aptamer will be selected to bind to a known peptide antigen. A biotinylated column will be utilized to stabilize a random library of DNA aptamers, and those peptides that bind to certain aptamers will cause a conformational change leading to the elution of those specific DNA aptamers. This SELEX process will be conducted again on the eluted aptamers to further select for strong binding DNA aptamers. The DNA aptamers that are obtained can further on be sequenced or used for prostate cancer research studies. Another possible usage of aptamers is to diagnose and determine the stage of various different cancer types. Our prediction is that this research will produce a DNA aptamer that will bind to a specific prostate cancer peptide antigen. | |
| Identifier: | CFH0004671 (IID), ucf:45294 (fedora) | |
| Note(s): |
2014-08-01 B.A. Medicine, Dept. of Molecular Biology and Microbiology Bachelors This record was generated from author submitted information. |
|
| Subject(s): |
SELEX aptamer DNA aptamers DNA PSA prostate specific antigen PSA1 PSA2 PSA3 in-vitro selection selection ligand Systemic Evolution of Ligands by Experimental Enrichment |
|
| Persistent Link to This Record: | http://purl.flvc.org/ucf/fd/CFH0004671 | |
| Restrictions on Access: | campus 2017-08-01 | |
| Host Institution: | UCF |
