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PARASYMPATHETIC NERVE DERIVED EXOSOMES INHIBIT HYPERGLYCEMIA INDUCED APOPTOSIS IN CARDIOMYOBLAST CELLS
| Title: | PARASYMPATHETIC NERVE DERIVED EXOSOMES INHIBIT HYPERGLYCEMIA INDUCED APOPTOSIS IN CARDIOMYOBLAST CELLS. |
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| Name(s): |
Singla, Reetish K, Author Cheng, Zixi (Jack), Committee Chair University of Central Florida, Degree Grantor |
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| Type of Resource: | text | |
| Date Issued: | 2018 | |
| Publisher: | University of Central Florida | |
| Language(s): | English | |
| Abstract/Description: | Diabetic cardiomyopathy involves both forms of cardiac cell cell death such as apoptosis and necrosis. However, this remains unknown whether hyperglycemia induced apoptosis in the cell culture system is inhibited by parasympathetic nerve derived exosomes. We isolated parasympathetic and sympathetic nerves and derived exosomes. We developed hyperglycemia induced apoptosis in H9c2 cells. H9c2 cells were divided into 4 groups: 1) Control, 2) H9c2+ Glucose 100 mmol, 3) H9c2+ Glucose +parasympathetic-exo, 4) H9c2+ Glucose+sympathetic-exo. We determined cell proliferation and viability with MTT assay kit and apoptosis with TUNEL staining and cell death detection ELISA kit. Data was further confirmed with pro-apoptotic proteins caspase-3 and BAX and anti-apoptotic protein Bcl2. High glucose exposed H9c2 cells significantly reduced cell viability which is improved by parasympathetic-exo but not by sympathetic-exo. Increased apoptosis in hyperglycemia in H9c2 cells confirmed with TUNEL staining and cell death ELISA was significantly (p | |
| Identifier: | CFH2000378 (IID), ucf:45833 (fedora) | |
| Note(s): |
2018-08-01 B.S. College of Medicine, Burnett School of Biomedical Sciences Bachelors This record was generated from author submitted information. |
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| Subject(s): |
Diabetes Exosomes Apoptosis |
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| Persistent Link to This Record: | http://purl.flvc.org/ucf/fd/CFH2000378 | |
| Restrictions on Access: | campus 2023-08-01 | |
| Host Institution: | UCF |
