You are here

UCF Digital Collections ยป Honors Collection

APTAMERIC SENSORS: IN VITRO SELECTION OF DNA THAT BINDS BROMOCRESOL PURPLE

Download pdf | Full Screen View

Date Issued:
2016
Abstract/Description:
Aptamers being used as sensors is an emerging field that has capabilities of being tomorrow's diagnostic tools. As aptameric sensors have become more popular, their visualization systems have been limited. The majority of today's aptameric sensors require expensive machinery such as a fluorometer in order to visualize results. We propose a system that will cut the need for instrumentation and be detected via the naked eye. With the selection of an aptamer to bind the pH indicating dye bromocresol purple (BCP) this may be achieved. When rendered active, the binding towards BCP will facilitate a color change from yellow to purple or vice versa. Previous studies have shown albumin contains the ability to facilitate this role and we now intend to use a DNA aptamer to achieve this as well. The BCP aptamer has the potential to serve as a signaling domain to any already selected aptamer thus making it a universal tool for both research and diagnostic measures. We have found that an alternative structure-switching systematic evolution of ligands by exponential enrichment (SELEX) method which left the dye unaltered was not sufficient for selecting an aptamer. We believe that a classical SELEX will enable us to select an aptamer that may be used to accomplish this role as a universal visual detector.
Title: APTAMERIC SENSORS: IN VITRO SELECTION OF DNA THAT BINDS BROMOCRESOL PURPLE.
30 views
12 downloads
Name(s): Miller, Derek B, Author
Kolpshchikov, Dmitry, Committee Chair
University of Central Florida, Degree Grantor
Type of Resource: text
Date Issued: 2016
Publisher: University of Central Florida
Language(s): English
Abstract/Description: Aptamers being used as sensors is an emerging field that has capabilities of being tomorrow's diagnostic tools. As aptameric sensors have become more popular, their visualization systems have been limited. The majority of today's aptameric sensors require expensive machinery such as a fluorometer in order to visualize results. We propose a system that will cut the need for instrumentation and be detected via the naked eye. With the selection of an aptamer to bind the pH indicating dye bromocresol purple (BCP) this may be achieved. When rendered active, the binding towards BCP will facilitate a color change from yellow to purple or vice versa. Previous studies have shown albumin contains the ability to facilitate this role and we now intend to use a DNA aptamer to achieve this as well. The BCP aptamer has the potential to serve as a signaling domain to any already selected aptamer thus making it a universal tool for both research and diagnostic measures. We have found that an alternative structure-switching systematic evolution of ligands by exponential enrichment (SELEX) method which left the dye unaltered was not sufficient for selecting an aptamer. We believe that a classical SELEX will enable us to select an aptamer that may be used to accomplish this role as a universal visual detector.
Identifier: CFH2000112 (IID), ucf:45951 (fedora)
Note(s): 2016-12-01
B.S.
College of Medicine, Burnett School Of Biomedical Sciences
Bachelors
This record was generated from author submitted information.
Subject(s): Aptamer
SELEX
Bromocresol Purple
DNA
Universal Detection
Colorimetric Assay
Persistent Link to This Record: http://purl.flvc.org/ucf/fd/CFH2000112
Restrictions on Access: public
Host Institution: UCF

In Collections