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USING LOW-COHERENCE INTERFEROMETRY TO MONITOR CELL INVASION IN AN IN-VITRO MODEL SYSTEM

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Date Issued:
2017
Abstract/Description:
In an optically random system, such as naturally occurring and man-made media, light undergoes pronounced multiple scattering. This phenomenon has shown a remarkable potential in characterizing complex materials. In this regime, scattering occurs from each individual center of the scattering and independent scattering events lead to multiple light scattering. This phenomenon is often described as a random walk of photons and can be modeled in terms of a diffusion equation based on the radiative transfer theory. In this thesis, we used optical path-length spectroscopy (OPS), which is an experimental method to obtain the path-length probability density of the propagating light in multiple scattering media, with a low-coherence optical field to investigate the distribution of photon path lengths in a skin cell model system. This method is capable of measuring the transport mean free path of light in a highly scattering medium and depth-resolved profiles of the backscattered light. Our OPS experimental configuration is based on a fiber-optic Michelson interferometer geometry using single mode optical fibers. We performed OPS based on low-coherence interferometry (LCI) on three-dimensional organotypic models of esophageal cell invasion by measuring the optical path-length distribution of backscattered light in normal and invasive conditions. The optical path-length distribution of light waves inside the cell samples provides information on how a change in the extracellular matrix affects invasiveness of the esophageal cells and induction of signaling pathways. Also, we demonstrated the compatibility to study the structural changes during a two-week period for in vitro cell samples.
Title: USING LOW-COHERENCE INTERFEROMETRY TO MONITOR CELL INVASION IN AN IN-VITRO MODEL SYSTEM.
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Name(s): Davoudi Nasab, Behnaz, Author
Dogariu, Aristide, Committee Chair
Andl, Claudia, Committee Member
University of Central Florida, Degree Grantor
Type of Resource: text
Date Issued: 2017
Publisher: University of Central Florida
Language(s): English
Abstract/Description: In an optically random system, such as naturally occurring and man-made media, light undergoes pronounced multiple scattering. This phenomenon has shown a remarkable potential in characterizing complex materials. In this regime, scattering occurs from each individual center of the scattering and independent scattering events lead to multiple light scattering. This phenomenon is often described as a random walk of photons and can be modeled in terms of a diffusion equation based on the radiative transfer theory. In this thesis, we used optical path-length spectroscopy (OPS), which is an experimental method to obtain the path-length probability density of the propagating light in multiple scattering media, with a low-coherence optical field to investigate the distribution of photon path lengths in a skin cell model system. This method is capable of measuring the transport mean free path of light in a highly scattering medium and depth-resolved profiles of the backscattered light. Our OPS experimental configuration is based on a fiber-optic Michelson interferometer geometry using single mode optical fibers. We performed OPS based on low-coherence interferometry (LCI) on three-dimensional organotypic models of esophageal cell invasion by measuring the optical path-length distribution of backscattered light in normal and invasive conditions. The optical path-length distribution of light waves inside the cell samples provides information on how a change in the extracellular matrix affects invasiveness of the esophageal cells and induction of signaling pathways. Also, we demonstrated the compatibility to study the structural changes during a two-week period for in vitro cell samples.
Identifier: CFH2000219 (IID), ucf:45955 (fedora)
Note(s): 2017-05-01
B.S.
College of Sciences, Chemistry
Bachelors
This record was generated from author submitted information.
Subject(s): Low-coherence interferometry
Scatteirng in random media
Esophageal cancer
Persistent Link to This Record: http://purl.flvc.org/ucf/fd/CFH2000219
Restrictions on Access: public
Host Institution: UCF

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