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VALIDATING DRUG TARGETS THROUGH INHIBITION OF PROTEIN-PROTEIN INTERACTIONS IN MYCOBACTERIUM TUBERCULOSIS
| Title: | VALIDATING DRUG TARGETS THROUGH INHIBITION OF PROTEIN-PROTEIN INTERACTIONS IN MYCOBACTERIUM TUBERCULOSIS. |
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| Name(s): |
Driscoll, Erin C, Author Rohde, Kyle, Committee Chair University of Central Florida, Degree Grantor |
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| Type of Resource: | text | |
| Date Issued: | 2017 | |
| Publisher: | University of Central Florida | |
| Language(s): | English | |
| Abstract/Description: | Tuberculosis is the leading cause of death by single infectious disease worldwide; novel antibiotics are needed to continue to treat this disease. To goal of this project is to provide proof-of-principle support for the idea that targeting protein-protein interactions (PPI) is an appropriate course for the discovery of new drugs. This study optimized the M-PFC assay, which allows detection of PPI in Mycobacteria, through the use of stronger promoters and inducible expression of a peptide blocker by riboswitch. To accomplish this, promoter induction studies were used to find stronger promoters for the M-PFC, optimization of the riboswitch as a method for inducible protein expression within this system, and the addition of both elements to the existing version of the M-PFC. This M-PFC targets DosR homodimerization; this process is known to be essential for survival within the host. This study optimizes a system that may be used to screen for drugs that are capable of interrupting this interaction. | |
| Identifier: | CFH2000190 (IID), ucf:46030 (fedora) | |
| Note(s): |
2017-05-01 B.S. College of Medicine, Burnett School of Biomedical Sciences Bachelors This record was generated from author submitted information. |
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| Subject(s): |
tuberculosis mycobacteria M-PFC mycobacterium smegmatis riboswitch |
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| Persistent Link to This Record: | http://purl.flvc.org/ucf/fd/CFH2000190 | |
| Restrictions on Access: | campus 2022-05-01 | |
| Host Institution: | UCF |
