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EXPRESSION LEVELS OF VIRULENCE GENES IN GROUP A STREPTOCOCCI: A RESPONSE TO AEROSOLIZED PROPYLENE GLYCOL

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Date Issued:
2016
Abstract/Description:
Electronic cigarette usage is becoming increasingly prevalent among school age children and young adults. A known bactericidal agent, propylene glycol, is often used as a carrier for nicotine, flavoring, and additional constituents of electronic cigarette juice. This study examined the relationship between propylene glycol and virulence gene expression in Streptococcus pyogenes, a respiratory tract pathogen commonly found in school-age individuals. A variety of virulence genes controlled by the three stand alone regulators mga, RofA, and Rgg/RopB were sampled in an effort to understand the pathway by which virulence is affected. The genes chosen encode C5a peptidase, fibronectin binding protein, hyaluronate lyase, NAD glycohydrolase, Streptococcal pyrogenic exotoxin A and B, streptodornase, streptokinase, Streptolysin O, and Streptolysin S. No significant change in gene expression was observed, but a novel method to test the effects of aerosols on cells was developed. This method can be used in the future to observe the effect of aerosols, including commercial electronic cigarette juice, on both bacterial and mammalian cells.
Title: EXPRESSION LEVELS OF VIRULENCE GENES IN GROUP A STREPTOCOCCI: A RESPONSE TO AEROSOLIZED PROPYLENE GLYCOL.
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Name(s): Costello, Michael S, Author
Hoffman, Eric, Committee Chair
Moore, Sean D., Committee Member
University of Central Florida, Degree Grantor
Type of Resource: text
Date Issued: 2016
Publisher: University of Central Florida
Language(s): English
Abstract/Description: Electronic cigarette usage is becoming increasingly prevalent among school age children and young adults. A known bactericidal agent, propylene glycol, is often used as a carrier for nicotine, flavoring, and additional constituents of electronic cigarette juice. This study examined the relationship between propylene glycol and virulence gene expression in Streptococcus pyogenes, a respiratory tract pathogen commonly found in school-age individuals. A variety of virulence genes controlled by the three stand alone regulators mga, RofA, and Rgg/RopB were sampled in an effort to understand the pathway by which virulence is affected. The genes chosen encode C5a peptidase, fibronectin binding protein, hyaluronate lyase, NAD glycohydrolase, Streptococcal pyrogenic exotoxin A and B, streptodornase, streptokinase, Streptolysin O, and Streptolysin S. No significant change in gene expression was observed, but a novel method to test the effects of aerosols on cells was developed. This method can be used in the future to observe the effect of aerosols, including commercial electronic cigarette juice, on both bacterial and mammalian cells.
Identifier: CFH2000106 (IID), ucf:46048 (fedora)
Note(s): 2016-05-01
B.S.
College of Sciences, Biology
Bachelors
This record was generated from author submitted information.
Subject(s): Streptococcus Pyogenes
Virulence
Electronic Cigarette
Persistent Link to This Record: http://purl.flvc.org/ucf/fd/CFH2000106
Restrictions on Access: campus 2019-05-01
Host Institution: UCF

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