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BASIC STUDY OF MICROMACHINED DEP (DIELECTROPHORETIC) MANIPULATOR

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Date Issued:
2004
Abstract/Description:
The capability of manipulating microparticle in small volumes is fundamental to many biological and medical applications, including separation and detection of cells. The development of microtools for effective sample handling and separation in such volumes is still a challenge. DEP (dielectrophoresis) is one of the most widely used methods in handling the microparticles. In this thesis we show that forces generated by nonuniform electric field (DEP) can be used for trapping and separating the microparticles (latex beads). This work further explores the DEP force based on different electrode geometries and medium conductivity. A micromanipulator for latex bead separation was designed, fabricated and characterized. For the development of DEP manipulator, the fabrication and packaging of microfluidic structure with the microelectrode is crucial for reliable analysis. A combination of SU-8 photoresist and polydimethylsiloxane polymer was used for this purpose. Besides, the DEP manipulator, preliminary study on a Coulter counter was conducted. The Coulter counter works on the principle of resistive pulse sensing. This counter is used for counting the beads as they pass through the microfluidic channel. Its possible integration with the manipulator was also explored.
Title: BASIC STUDY OF MICROMACHINED DEP (DIELECTROPHORETIC) MANIPULATOR.
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Name(s): Sundaram, Vivek, Author
, K.B.Sundaram, Committee Chair
University of Central Florida, Degree Grantor
Type of Resource: text
Date Issued: 2004
Publisher: University of Central Florida
Language(s): English
Abstract/Description: The capability of manipulating microparticle in small volumes is fundamental to many biological and medical applications, including separation and detection of cells. The development of microtools for effective sample handling and separation in such volumes is still a challenge. DEP (dielectrophoresis) is one of the most widely used methods in handling the microparticles. In this thesis we show that forces generated by nonuniform electric field (DEP) can be used for trapping and separating the microparticles (latex beads). This work further explores the DEP force based on different electrode geometries and medium conductivity. A micromanipulator for latex bead separation was designed, fabricated and characterized. For the development of DEP manipulator, the fabrication and packaging of microfluidic structure with the microelectrode is crucial for reliable analysis. A combination of SU-8 photoresist and polydimethylsiloxane polymer was used for this purpose. Besides, the DEP manipulator, preliminary study on a Coulter counter was conducted. The Coulter counter works on the principle of resistive pulse sensing. This counter is used for counting the beads as they pass through the microfluidic channel. Its possible integration with the manipulator was also explored.
Identifier: CFE0000121 (IID), ucf:46178 (fedora)
Note(s): 2004-08-01
M.S.E.E.
College of Engineering and Computer Science, Department of Electrical and Computer Engineering
This record was generated from author submitted information.
Subject(s): dielectrophoresis
coulter counter
interdigitated electrodes
SU-8
PDMS
Persistent Link to This Record: http://purl.flvc.org/ucf/fd/CFE0000121
Restrictions on Access: campus 2009-01-31
Host Institution: UCF

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