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NOTOPLEURAL MUTATIONS ENHANCE DEFECTS IN IMAGINAL DISC EPITHELIAL MORPHOGENESIS AND MACROCHETE ELONGATION ASSOCIATED WITH MUTATIONS IN THE STUBBLE-STUBBLOID LOCUS
| Title: | NOTOPLEURAL MUTATIONS ENHANCE DEFECTS IN IMAGINAL DISC EPITHELIAL MORPHOGENESIS AND MACROCHETE ELONGATION ASSOCIATED WITH MUTATIONS IN THE STUBBLE-STUBBLOID LOCUS. |
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| Name(s): |
Ruggiero, Robert, Author von Kalm, Laurence, Committee Chair University of Central Florida, Degree Grantor |
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| Type of Resource: | text | |
| Date Issued: | 2006 | |
| Publisher: | University of Central Florida | |
| Language(s): | English | |
| Abstract/Description: | The Stubble-stubbloid locus encodes a transmembrane serine protease (Stubble) necessary for the proper formation of sensory bristles, and the morphogenesis of leg and wing epithelia. Genetic and cell biological analysis indicate a role for Stubble in actin cytoskeletal dynamics and cell shape changes in developing epithelia and bristles. Previously reported genetic interactions between Stubble and the Rho1 signaling pathway suggest Stubble influences actin cytoskeleton dynamics in developing imaginal discs through interactions with the Rho1 pathway. This work will discuss a genetic screen conducted to further investigate the role of Stubble in bristle and imaginal disc morphogenesis. From 50,000 EMS-mutagenized chromosomes 12 enhancers of the recessive sbd201 allele were identified, including 6 new sbd alleles. Consistent with the current understanding of genetic interactions regulating imaginal disc morphogenesis, mutations in two Rho1 pathway genes, zipper (2 alleles) and Rho1, were isolated. Additionally, three new mutant enhancers of sbd201 were isolated, one of which has been identified as an allele of the cadherin gene Dacshous, another as an allele of the muscle myosin heavy chain gene, and the last as an allele of Notopleural (Np). Dominant and recessive mutations in the Stubble locus interact with the Np allele identified in this screen, in regards to both limb and bristle development, respectively. Mutations in the Np locus were first identified in 1936, but this locus remains poorly characterized and has never been cloned The genetic and phenotypic characterization of Np will be discussed along with experiments that have mapped the position of the Np locus to a 50kb region at the border of the 44F12, 45A1 cytological regions. | |
| Identifier: | CFE0001347 (IID), ucf:46986 (fedora) | |
| Note(s): |
2006-12-01 M.S. Sciences, Department of Biology Masters This record was generated from author submitted information. |
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| Subject(s): |
Epithelial morphogenesis Drosophila imaginal disc metamorphosis Rho1 Stubble Notopleural |
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| Persistent Link to This Record: | http://purl.flvc.org/ucf/fd/CFE0001347 | |
| Restrictions on Access: | public | |
| Host Institution: | UCF |
