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CHROMATOGRAPHIC AND MASS SPECTRAL ANALYSES OF OLIGOSACCHARIDES AND INDIGO DYE EXTRACTED FROM COTTON TEXTILES WITH MANOVA AND ANOVA STATISTICAL DATA ANALYSES
| Title: | CHROMATOGRAPHIC AND MASS SPECTRAL ANALYSES OF OLIGOSACCHARIDES AND INDIGO DYE EXTRACTED FROM COTTON TEXTILES WITH MANOVA AND ANOVA STATISTICAL DATA ANALYSES. |
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| Name(s): |
Frisch, Jessica, Author Sigman, Michael, Committee Chair University of Central Florida, Degree Grantor |
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| Type of Resource: | text | |
| Date Issued: | 2008 | |
| Publisher: | University of Central Florida | |
| Language(s): | English | |
| Abstract/Description: | Research was conducted on thirteen 100% cotton denim samples using an acid wash, established by Murray, to extract oligosaccharides from the cellulosic material. The oligosaccharide ion groups (+, +, and +) for molecules with degrees of polymerization between two and seven (DP2-DP7) were analyzed using liquid chromatography coupled to mass spectrometry with an electrospray ionization interface (LC-ESI-MS). The results were compared using the least-squares means in a Multivariate ANOVA (MANOVA) test followed by Univariate ANOVA and Tukey HSD tests and demonstrated that the method could correctly determine that two samples were statistically different 85.9% of the time when analyzing the amount (ng) of each of the oligosaccharide ion groups separately, and 82.0% when analyzing the total moles of monosaccharide units released. A dye extraction was performed on the denim materials and the extract analyzed using gas chromatography coupled with mass spectrometry (GC-MS). Indigo dye was present in all of the denim samples except one. When these results were combined with the two oligosaccharide statistical analyses, the discriminating power was increased to 88.5% and 85.9%, respectively. Additional cellulosic materials were also investigated including four white 100% cotton t-shirts as well as five raw cotton samples grown in Tajikistan, Uzbekistan, Egypt, Iran, and Benin West Africa. The analytical methodology gave results for the white cotton t-shirts and raw cotton samples that were inconsistent with those obtained from the denim samples. | |
| Identifier: | CFE0002068 (IID), ucf:47583 (fedora) | |
| Note(s): |
2008-05-01 M.S. Sciences, Department of Chemistry Masters This record was generated from author submitted information. |
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| Subject(s): |
cellulose cotton forensic indigo lc-esi-ms oligosaccharides |
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| Persistent Link to This Record: | http://purl.flvc.org/ucf/fd/CFE0002068 | |
| Restrictions on Access: | campus 2009-04-01 | |
| Host Institution: | UCF |
