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SURFACE ENTROPY REDUCTION TO INCREASE THE CRYSTALLIZABILITY OF THE FAB-RNA COMPLEX

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Date Issued:
2011
Abstract/Description:
Crystallizing RNA has been an imperative facet and a challenging task in the world of RNA research. Assistive methods such as Chaperone Assisted RNA Crystallography (CARC), employing monoclonal antibody fragments (Fabs) as crystallization chaperones have enabled us to obtain RNA crystal structures by increasing the crystal contacts and providing initial phasing information. Using this technology the crystal structure of ΔC209 P4-P6 RNA (an independent folding domain of the self-splicing Tetrahymena group I intron) complexed to Fab2 (high affinity binding Fab) has been resolved to 1.95 Å. Although the complexed class I ligase ribozyme has also been crystallized using CARC, in practice, it has been found that the crystallization of, large RNA-Fab complex remains a confrontation. The possible reason for this difficulty is that Fabs have not been optimized for crystallization when complexed with RNA. Here we have used the Surface Entropy Reduction technique (SER) for the optimization process. Candidate residues for mutations were identified based on combining results from visual inspection of ΔC209 P4-P6/Fab2 crystal structure complex using pyMOL software and a web- based SER software. The protruding lysine and glutamate residues were mutated to a set of alanine (Super Mutant Alanine SMA) and serine (Super Mutant Serine SMS) mutant clones. Filter binding assay studies confirmed that the mutant clones bind to ΔC209 P4-P6 with similar binding affinities as that of the parent Fab2. Large scale expression of the mutants, parent clone and ΔC209 P4-P6 RNA were optimised. Crystal trays for ΔC209 P4-P6 complexed with Fab2, Fab2SMA and Fab2SMS were set-up side-by-side using Hampton crystal screen kits and ~600 conditions including temperature as a variable condition were screened. Crystal screening shows significantly higher crystal-forming ratios for the mutant complexes. As the chosen SER residues are far away from the CDR regions of the Fab, the same set of mutations can be potentially applied to other Fabs binding to a variety of ribozymes and riboswitches to improve the crystallizability of the Fab-RNA complex.
Title: SURFACE ENTROPY REDUCTION TO INCREASE THE CRYSTALLIZABILITY OF THE FAB-RNA COMPLEX.
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Name(s): P.Ravindran, Priyadarshini, Author
Ye, Jingdong, Committee Chair
University of Central Florida, Degree Grantor
Type of Resource: text
Date Issued: 2011
Publisher: University of Central Florida
Language(s): English
Abstract/Description: Crystallizing RNA has been an imperative facet and a challenging task in the world of RNA research. Assistive methods such as Chaperone Assisted RNA Crystallography (CARC), employing monoclonal antibody fragments (Fabs) as crystallization chaperones have enabled us to obtain RNA crystal structures by increasing the crystal contacts and providing initial phasing information. Using this technology the crystal structure of ΔC209 P4-P6 RNA (an independent folding domain of the self-splicing Tetrahymena group I intron) complexed to Fab2 (high affinity binding Fab) has been resolved to 1.95 Å. Although the complexed class I ligase ribozyme has also been crystallized using CARC, in practice, it has been found that the crystallization of, large RNA-Fab complex remains a confrontation. The possible reason for this difficulty is that Fabs have not been optimized for crystallization when complexed with RNA. Here we have used the Surface Entropy Reduction technique (SER) for the optimization process. Candidate residues for mutations were identified based on combining results from visual inspection of ΔC209 P4-P6/Fab2 crystal structure complex using pyMOL software and a web- based SER software. The protruding lysine and glutamate residues were mutated to a set of alanine (Super Mutant Alanine SMA) and serine (Super Mutant Serine SMS) mutant clones. Filter binding assay studies confirmed that the mutant clones bind to ΔC209 P4-P6 with similar binding affinities as that of the parent Fab2. Large scale expression of the mutants, parent clone and ΔC209 P4-P6 RNA were optimised. Crystal trays for ΔC209 P4-P6 complexed with Fab2, Fab2SMA and Fab2SMS were set-up side-by-side using Hampton crystal screen kits and ~600 conditions including temperature as a variable condition were screened. Crystal screening shows significantly higher crystal-forming ratios for the mutant complexes. As the chosen SER residues are far away from the CDR regions of the Fab, the same set of mutations can be potentially applied to other Fabs binding to a variety of ribozymes and riboswitches to improve the crystallizability of the Fab-RNA complex.
Identifier: CFE0003944 (IID), ucf:48711 (fedora)
Note(s): 2011-08-01
M.S.
Sciences, Burnett College of Biomedical Sciences
Masters
This record was generated from author submitted information.
Subject(s): Chaperone Assisted RNA Crystallography
Fab
protein engineering
shake flask expression
Surface Entropy Reduction
Persistent Link to This Record: http://purl.flvc.org/ucf/fd/CFE0003944
Restrictions on Access: campus 2014-07-01
Host Institution: UCF

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