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Modeling Transport and Protein Adsorption in Microfluidic Systems

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Date Issued:
2011
Abstract/Description:
This work describes theoretical advances in the modeling and simulation of microfluidic systems and demonstrates the practical application of those techniques. A new multi-scale model of the adsorption of hard spheres was formulated to bridge the gap between simulations of discrete particles and continuum fluid dynamics. A whispering gallery mode (WGM) biosensor was constructed and used to measure the kinetics of adsorption for two types of proteins on four different surfaces. Computational fluid dynamics was used to analyze the transport of proteins in the flow cell of the biosensor. Kinetic models of protein adsorption that take transport limitations into account were fitted to the experimental data and used to draw conclusions about the mechanisms of adsorption. Transport simulations were then applied to the practical problem of optimizing the design of a microfluidic bioreactor to enable (")plugs(") of fluid to flow from one chamber to the next with minimal dispersion. Experiments were used to validate the transport simulations. The combination of quantitative modeling and simulation and experiments led to results that could not have been achieved using either approach by itself. Simulation tools that accurately predict transport and protein adsorption will enable the rational design of microfluidic devices for biomedical applications.
Title: Modeling Transport and Protein Adsorption in Microfluidic Systems.
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Name(s): Finch, Craig, Author
Hickman, James, Committee Chair
Kincaid, John, Committee CoChair
Lin, Kuo-Chi, Committee Member
Behal, Aman, Committee Member
Cho, Hyoung, Committee Member
University of Central Florida, Degree Grantor
Type of Resource: text
Date Issued: 2011
Publisher: University of Central Florida
Language(s): English
Abstract/Description: This work describes theoretical advances in the modeling and simulation of microfluidic systems and demonstrates the practical application of those techniques. A new multi-scale model of the adsorption of hard spheres was formulated to bridge the gap between simulations of discrete particles and continuum fluid dynamics. A whispering gallery mode (WGM) biosensor was constructed and used to measure the kinetics of adsorption for two types of proteins on four different surfaces. Computational fluid dynamics was used to analyze the transport of proteins in the flow cell of the biosensor. Kinetic models of protein adsorption that take transport limitations into account were fitted to the experimental data and used to draw conclusions about the mechanisms of adsorption. Transport simulations were then applied to the practical problem of optimizing the design of a microfluidic bioreactor to enable (")plugs(") of fluid to flow from one chamber to the next with minimal dispersion. Experiments were used to validate the transport simulations. The combination of quantitative modeling and simulation and experiments led to results that could not have been achieved using either approach by itself. Simulation tools that accurately predict transport and protein adsorption will enable the rational design of microfluidic devices for biomedical applications.
Identifier: CFE0004474 (IID), ucf:49313 (fedora)
Note(s): 2011-12-01
Ph.D.
Sciences, Psychology
Doctoral
This record was generated from author submitted information.
Subject(s): microfluidics -- surface chemistry -- adsorption -- computational fluid dynamics -- modeling -- simulation -- bioreactor
Persistent Link to This Record: http://purl.flvc.org/ucf/fd/CFE0004474
Restrictions on Access: public 2012-06-15
Host Institution: UCF

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