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Fabrication and Investigation of an enzyme-free, Nanoparticle-based Biosensor for Hydrogen Peroxide determination

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Date Issued:
2016
Abstract/Description:
Electrochemical biosensors often employ enzymes as detection elements. These sensors are highly selective towards target analytes, however the scope of their application is limited by the poor stability of the enzyme. In this study, multi-valent inorganic cerium oxide nanoparticles were used as detection elements for the analysis of hydrogen peroxide. The electrochemical response of the cerium oxide towards hydrogen peroxide analyte is defined through cyclic voltammetry and chronoamperometry. This response was found to be dependent on nanoparticle Ce3+:Ce4+ redox state ratio and this property is exploited to fabricate a biosensor. As produced, the biosensor demonstrated sensitivity at picomolar analyte concentrations. Further, the sensitivity of the electrode is stable across a range of temperatures and pH's which inhibit the function of standard enzyme-based sensors. Additionally, the produced sensor retained function in sheep serum demonstrating the high selectivity and robustness of the sensor.
Title: Fabrication and Investigation of an enzyme-free, Nanoparticle-based Biosensor for Hydrogen Peroxide determination.
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Name(s): Neal, Craig, Author
Seal, Sudipta, Committee Chair
Cho, Hyoung Jin, Committee Member
Florczyk, Stephen, Committee Member
University of Central Florida, Degree Grantor
Type of Resource: text
Date Issued: 2016
Publisher: University of Central Florida
Language(s): English
Abstract/Description: Electrochemical biosensors often employ enzymes as detection elements. These sensors are highly selective towards target analytes, however the scope of their application is limited by the poor stability of the enzyme. In this study, multi-valent inorganic cerium oxide nanoparticles were used as detection elements for the analysis of hydrogen peroxide. The electrochemical response of the cerium oxide towards hydrogen peroxide analyte is defined through cyclic voltammetry and chronoamperometry. This response was found to be dependent on nanoparticle Ce3+:Ce4+ redox state ratio and this property is exploited to fabricate a biosensor. As produced, the biosensor demonstrated sensitivity at picomolar analyte concentrations. Further, the sensitivity of the electrode is stable across a range of temperatures and pH's which inhibit the function of standard enzyme-based sensors. Additionally, the produced sensor retained function in sheep serum demonstrating the high selectivity and robustness of the sensor.
Identifier: CFE0006362 (IID), ucf:51540 (fedora)
Note(s): 2016-08-01
M.S.M.S.E.
Engineering and Computer Science, Materials Science Engineering
Masters
This record was generated from author submitted information.
Subject(s): Biosensor
Ceria
Reactive Oxygen Species
Nanotechnology
Enzyme-Free
Persistent Link to This Record: http://purl.flvc.org/ucf/fd/CFE0006362
Restrictions on Access: public 2016-08-15
Host Institution: UCF

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