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Fabrication of Polyelectrolyte Nanoparticles Through Hydrophobic Interaction

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Date Issued:
2019
Abstract/Description:
Anticancer drugs like gemcitabine (GEM) are used to treat cancers such as, pancreatic ductal adenocarcinoma (PDAC). However, the use of free gemcitabine yields challenges including cytotoxicity to healthy cells and poor circulation time. By encapsulating GEM in nanoparticles these challenges can be overcome. In this study poly(acrylic acid) (PAA)-GEM nanoparticles are fabricated by coupling GEM onto PAA. The particle formation is driven by the hydrophobic interaction of GEM, which collects in the core of the nanoparticle, forming a PAA shell. The nanoparticles were optimized by studying the PAA/GEM ratio and pH during fabrication. Characteristics of the nanoparticles including size, morphology and surface charge were investigated using dynamic light scattering (DLS), transmission electron microscopy (TEM) and zeta potential measurements. Conditions such as ionic stability and pH stability were optimized to achieve high drug loading efficiency. Cell uptake and cytotoxicity studies were used to determine the efficiency of the nanoparticles as drug delivery vehicle.
Title: Fabrication of Polyelectrolyte Nanoparticles Through Hydrophobic Interaction.
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Name(s): Catarata, Ruginn Porce, Author
Zhai, Lei, Committee Chair
Kang, Ellen, Committee Member
Huo, Qun, Committee Member
University of Central Florida, Degree Grantor
Type of Resource: text
Date Issued: 2019
Publisher: University of Central Florida
Language(s): English
Abstract/Description: Anticancer drugs like gemcitabine (GEM) are used to treat cancers such as, pancreatic ductal adenocarcinoma (PDAC). However, the use of free gemcitabine yields challenges including cytotoxicity to healthy cells and poor circulation time. By encapsulating GEM in nanoparticles these challenges can be overcome. In this study poly(acrylic acid) (PAA)-GEM nanoparticles are fabricated by coupling GEM onto PAA. The particle formation is driven by the hydrophobic interaction of GEM, which collects in the core of the nanoparticle, forming a PAA shell. The nanoparticles were optimized by studying the PAA/GEM ratio and pH during fabrication. Characteristics of the nanoparticles including size, morphology and surface charge were investigated using dynamic light scattering (DLS), transmission electron microscopy (TEM) and zeta potential measurements. Conditions such as ionic stability and pH stability were optimized to achieve high drug loading efficiency. Cell uptake and cytotoxicity studies were used to determine the efficiency of the nanoparticles as drug delivery vehicle.
Identifier: CFE0007791 (IID), ucf:52364 (fedora)
Note(s): 2019-12-01
M.S.
Graduate Studies,
Masters
This record was generated from author submitted information.
Subject(s): Nanoparticles -- polyelectrolytes -- hydrogel -- drug delivery -- pancreatic cancer
Persistent Link to This Record: http://purl.flvc.org/ucf/fd/CFE0007791
Restrictions on Access: campus 2020-12-15
Host Institution: UCF

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