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The effects of enzymes on activated sludge floc

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Date Issued:
1977
Abstract/Description:
Florida Technological University College of Natural Sciences Thesis; Dialysed activated sludge was used as a substrate to test for enzymes which can hydrolyse activated sludge floc. Two hundred and fifty aerobic and anaerobic microorganisms were tested against activated sludge for the presence of hydrolytic enzymes. These included known genera and organisms obtained by various enrichment procedures. Anaerobic digester mixed liquor was contacted with activated sludge agar under anaerobic conditions. None of the microorganisms present in the digester liquor hydrolysed the floc. The following commercial enzymes were contacted with activated sludge singly, in combination, and sequentially under various physical and chemical conditions: protease, lipase, cellulase, pectinase, phospholipase C, trypsin, and glucuronidase. Although commercial enzymes and various microorganisms reacted with known substrate controls neither the enzymes nor microorganisms employed affected the activated sludge floc. Treatment of activated sludge floc with ethylenediaminetetraacetic acid resulted in gross deflocculation and release of humic substances. The floc particle, probably a combination of polymers bound in a complex manner, is resistant to enzymatic degradation. Several workers have reported on the enzymatic hydrolysis of polymers produced by axenic cultures isolated from activated sludge floc. It is unlikely that the results of their work can be extrapolated to the complex structure of activated sludge floc particles.
Title: The effects of enzymes on activated sludge floc.
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Name(s): Stoddart, Terry Lee, Author
null, null, Committee Chair
Natural Sciences, Degree Grantor
Type of Resource: text
Date Issued: 1977
Publisher: Florida Technological University
Language(s): English
Abstract/Description: Florida Technological University College of Natural Sciences Thesis; Dialysed activated sludge was used as a substrate to test for enzymes which can hydrolyse activated sludge floc. Two hundred and fifty aerobic and anaerobic microorganisms were tested against activated sludge for the presence of hydrolytic enzymes. These included known genera and organisms obtained by various enrichment procedures. Anaerobic digester mixed liquor was contacted with activated sludge agar under anaerobic conditions. None of the microorganisms present in the digester liquor hydrolysed the floc. The following commercial enzymes were contacted with activated sludge singly, in combination, and sequentially under various physical and chemical conditions: protease, lipase, cellulase, pectinase, phospholipase C, trypsin, and glucuronidase. Although commercial enzymes and various microorganisms reacted with known substrate controls neither the enzymes nor microorganisms employed affected the activated sludge floc. Treatment of activated sludge floc with ethylenediaminetetraacetic acid resulted in gross deflocculation and release of humic substances. The floc particle, probably a combination of polymers bound in a complex manner, is resistant to enzymatic degradation. Several workers have reported on the enzymatic hydrolysis of polymers produced by axenic cultures isolated from activated sludge floc. It is unlikely that the results of their work can be extrapolated to the complex structure of activated sludge floc particles.
Identifier: CFR0003513 (IID), ucf:53004 (fedora)
Note(s): 1977-01-01
M.S.
Biological Science
Masters
This record was generated from author submitted information.
Electronically reproduced by the University of Central Florida from a book held in the John C. Hitt Library at the University of Central Florida, Orlando.
Subject(s): Sewage -- Flocculation
Sewage sludge -- Analysis
Persistent Link to This Record: http://purl.flvc.org/ucf/fd/CFR0003513
Restrictions on Access: public
Host Institution: UCF

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