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STRUCTURE-FUNCTION ANALYSIS OF THE DROSOPHILA STUBBLE TYPE II TRANSMEMBRANE SERINE PROTEASE
Title
STRUCTURE-FUNCTION ANALYSIS OF THE DROSOPHILA STUBBLE TYPE II TRANSMEMBRANE SERINE PROTEASE.
Creator
Morgan, Rachel, von Kalm, Laurence, University of Central Florida
Abstract / Description
Hormonally-triggered regulatory hierarchies play a major role in organismal development. Disruption of a single member of such a hierarchy can lead to irregular development and disease. Therefore, knowledge of the members involved and the mechanisms controlling signaling through such pathways is of great importance in understanding how resulting developmental defects occur. Type II transmembrane serine proteases (TTSPs) make up a family of cell surface-associated proteases that play important...
Show moreHormonally-triggered regulatory hierarchies play a major role in organismal development. Disruption of a single member of such a hierarchy can lead to irregular development and disease. Therefore, knowledge of the members involved and the mechanisms controlling signaling through such pathways is of great importance in understanding how resulting developmental defects occur. Type II transmembrane serine proteases (TTSPs) make up a family of cell surface-associated proteases that play important roles in the development and homeostasis of a number of mammalian tissues. Aberrant expression of TTSPs is linked to several human disorders, including deafness, heart and respiratory disease and cancer. However, the mechanism by which these proteases function remains unknown. The ecdysone-responsive Stubble TTSP of Drosophila serves as a good model in which to study the functional mechanism of the TTSP family. The Stubble protease interacts with the intracellular Rho1 (RhoA) pathway to control epithelial development in imaginal discs. The Rho1 signaling pathway regulates cellular behavior via control of gene expression and actin cytoskeletal dynamics. However, the mechanism by which the Stubble protease interacts with the Rho1 pathway to control epithelial development, in particular leg imaginal disc morphogenesis, has yet to be elucidated. The Stubble protein consists of several conserved domains. One approach to a better understanding of the mechanism of action of Stubble in regulating Rho1 signaling is to define which of the conserved domains within the protease are required for proper function. Sequence analysis of twelve recessive Stubble mutant alleles has revealed that the proteolytic domain is essential for proper function. Alleles containing mutations which disrupt regions of the protease domain necessary for protease activation or substrate binding, as well as those with deletions or truncations that remove some portion of the proteolytic domain, result in defective epithelial development in vivo. In contrast, mutations in other regions of the Stubble protein, including the disulfide-knotted and cytoplasmic domains, were not observed. Another important step for defining the connection between Stubble and Rho1 signaling is to identify a Stubble target that acts as an upstream regulator of the Rho1 pathway. We performed a genetic screen in which 97 of the 147 Drosophila non-olfactory and non-gustatory G-protein-coupled receptors (GPCRs), a family of proteins that has been shown to be protease-activated and to activate Rho1 signaling, were tested for interactions with a mutant allele of Stubble. We found 4 genomic regions uncovering a total of 7 GPCRs that interact genetically when in heterozygous combination with a Stubble mutant. Further analysis of these genes is necessary to determine if any of these GPCRs is targeted by Stubble during activation of the Rho1 pathway.
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Date Issued
2008
Identifier
CFE0002285, ucf:47875
Format
Document (PDF)
PURL
http://purl.flvc.org/ucf/fd/CFE0002285
VICTIMIZATION, RISKY BEHAVIORS, AND THE VIRTUAL WORLD
Title
VICTIMIZATION, RISKY BEHAVIORS, AND THE VIRTUAL WORLD.
Creator
Morgan, Rachel, Jasinski, Jana, University of Central Florida
Abstract / Description
Social networking sites, such as Facebook and MySpace, have become increasingly popular among teens and young adults because of the availability of the internet. Because these websites promote interpersonal connections and information sharing among individuals around the world, personal information to online "friends" may be shared carelessly. However, little is known about the correlation between engaging in online activities, sharing personal information online, and susceptibility to online...
Show moreSocial networking sites, such as Facebook and MySpace, have become increasingly popular among teens and young adults because of the availability of the internet. Because these websites promote interpersonal connections and information sharing among individuals around the world, personal information to online "friends" may be shared carelessly. However, little is known about the correlation between engaging in online activities, sharing personal information online, and susceptibility to online victimization and cyberbullying. This study analyzes data from the Parents & Teens 2006 Survey to examine the applicability of Routine Activities Theory as a theoretical framework for understanding cybervictimization and cyberbullying. Online teens and teens on social networking sites (SNS) were examined separately in this study to determine if social networking (SNS) teens were at an increased risk. The results indicated that participating in online activities and sharing personal information increased the risk for receiving a threatening email, instant message or text message. Teens whose parents did not have rules regulating their online activities and behaviors were also at an increased risk for receiving a threatening email, instant message or text message. The logistic regression models show that for social networking (SNS) teens, gender and age increase the odds of receiving a threat, compared to online teens.
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Date Issued
2010
Identifier
CFE0003048, ucf:48348
Format
Document (PDF)
PURL
http://purl.flvc.org/ucf/fd/CFE0003048
Tracking Violence: Using Neighborhood-Level Characteristics in the Analysis of Domestic Violence in Chicago and the State of Illinois
Title
Tracking Violence: Using Neighborhood-Level Characteristics in the Analysis of Domestic Violence in Chicago and the State of Illinois.
Creator
Morgan, Rachel, Jasinski, Jana, Lynxwiler, John, Mustaine, Elizabeth, University of Central Florida
Abstract / Description
Social disorganization theory proposes that neighborhood characteristics, such as residential instability, racial and ethnic heterogeneity, concentrated disadvantage, and immigrant concentration contribute to an increase in crime rates. Informal social controls act as a mediator between these neighborhood characteristics and crime and delinquency. Informal social controls are regulated by members of a community and in a disorganized community these controls are not present, therefore, crime...
Show moreSocial disorganization theory proposes that neighborhood characteristics, such as residential instability, racial and ethnic heterogeneity, concentrated disadvantage, and immigrant concentration contribute to an increase in crime rates. Informal social controls act as a mediator between these neighborhood characteristics and crime and delinquency. Informal social controls are regulated by members of a community and in a disorganized community these controls are not present, therefore, crime and delinquency flourish (Sampson, 2012). Researchers have focused on these measures of social disorganization and the ability to explain a variety of crimes, specifically public crimes. Recently, researchers have focused their attention to characteristics of socially disorganized areas and the ability to predict private crimes, such as domestic violence. This study contributes to the research on social disorganization theory and domestic violence by examining domestic offenses at three different units of analysis: Chicago census tracts, Chicago neighborhoods, and Illinois counties. Demographic variables from the 2005-2009 American Community Survey were utilized to measure social disorganization within Chicago census tracts, Chicago neighborhoods, and Illinois counties. Data on domestic offenses in Chicago were from the City of Chicago Data Portal and data on domestic offenses in Illinois counties were retrieved from the Illinois Criminal Justice Information Authority (ICJIA). This study incorporated geographic information systems (GIS) mapping to examine the relationships between locations of domestic offenses and the measures of social disorganization in each unit of analysis. Results of this study indicate that different measures of social disorganization are significantly associated with domestic offenses in each unit of analysis.
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Date Issued
2013
Identifier
CFE0004726, ucf:49832
Format
Document (PDF)
PURL
http://purl.flvc.org/ucf/fd/CFE0004726