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Intramuscular TNF-alpha signaling in response to resistance exercise and recovery in untrained males.
Title
Intramuscular TNF-alpha signaling in response to resistance exercise and recovery in untrained males.
Creator
Townsend, Jeremy, Stout, Jeffrey, Hoffman, Jay, Fukuda, David, Roberts, Michael, University of Central Florida
Abstract / Description
INTRODUCTION: The nuclear factor kappa B (NF-??) signaling cascade is responsible for mediating stress-activated catabolic effects of Tumor Necrosis Factor Alpha (TNF-?) downstream of the TNF receptor (TNFR1). NF-?? transcription factor remains inhibited in the cytosol of the muscle and can be stimulated for translocation and transcription by a variety of external stimuli, most notably by pro-inflammatory cytokines and oxidative stress. Nevertheless, currently there is a gap in the literature...
Show moreINTRODUCTION: The nuclear factor kappa B (NF-??) signaling cascade is responsible for mediating stress-activated catabolic effects of Tumor Necrosis Factor Alpha (TNF-?) downstream of the TNF receptor (TNFR1). NF-?? transcription factor remains inhibited in the cytosol of the muscle and can be stimulated for translocation and transcription by a variety of external stimuli, most notably by pro-inflammatory cytokines and oxidative stress. Nevertheless, currently there is a gap in the literature with regard to the time course effect of NF-?? signaling following acute resistance exercise in humans. PURPOSE: To observe the effects of an acute lower-body resistance exercise protocol and subsequent recovery on intramuscular NF- ?? signaling. METHODS: Twenty-eight untrained males were assigned to either a control (CON; n=11) or exercise group (EX; n=17) and completed a lower-body resistance exercise protocol consisting of the back squat, leg press, and leg extension exercises. Skeletal muscle microbiopsies were obtained from the vastus lateralis pre-exercise (PRE), 1-hour (1HR), 5-hour (5HR), and 48-hours (48HR) post-resistance exercise. Multiplex signaling assay kits (EMD Millipore, Billerica, MA, USA) were used to quantify the total protein (TNFR1, c-Myc) or phosphorylation status of proteins (IKKa/b, NF- ??, IkB) specific to apoptotic signaling pathways using MAGPIX(&)#174; (Luminex, Austin, TX, USA). Repeated measures ANOVA analysis was used to determine the effects of the exercise bout on intramuscular signaling at each timepoint. Additionally, change scores were analyzed by magnitude based inferences to determine a mechanistic interpretation. RESULTS: Repeated measures ANOVA indicated a trend for a two way interaction between the EX and CON Group (p=0.064). Magnitude based inferences revealed a (")Very Likely(") increase in total c-Myc from PRE-5H and a (")Likely(") increase in IkB phosphorylation from PRE-5H. CONCLUSION: Results indicate that c-Myc transcription factor and phosphorylation of IkB are elevated following acute intense resistance exercise in untrained males. These data suggest that NF- ?? signaling plays a role in ribosome biogenesis and skeletal muscle regeneration following resistance exercise in young untrained males.
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Date Issued
2016
Identifier
CFE0006191, ucf:51089
Format
Document (PDF)
PURL
http://purl.flvc.org/ucf/fd/CFE0006191
Sleep Disturbances Among Firefighters: The Impact Of Shift Work On Sleep And Cognition
Title
Sleep Disturbances Among Firefighters: The Impact Of Shift Work On Sleep And Cognition.
Creator
Stout, Jeremy, Beidel, Deborah, Bowers, Clint, Paulson, Daniel, Dziegielewski, Sophia, University of Central Florida
Abstract / Description
A comprehensive sleep assessment of 45 firefighters was conducted over 9- days in an effort to determine the impact of their 24 hour work and 48 hour off work schedule on their sleep duration, sleep quality, processing speed, sustained attention, vigilance, and mental health. Chronic patterns of poor sleep are associated with an increased likelihood of performing poorly on tasks that require processing speed and sustained attention/vigilance which could lead to firefighters' suboptimal work...
Show moreA comprehensive sleep assessment of 45 firefighters was conducted over 9- days in an effort to determine the impact of their 24 hour work and 48 hour off work schedule on their sleep duration, sleep quality, processing speed, sustained attention, vigilance, and mental health. Chronic patterns of poor sleep are associated with an increased likelihood of performing poorly on tasks that require processing speed and sustained attention/vigilance which could lead to firefighters' suboptimal work performance or an increased risk of injury. Firefighters completed sleep actigraphy, self- report measures, as well as neuropsychological sub-tests at their beginning of their shift and immediately at the end of their work shift. As measured by actigraphy, firefighters in this sample slept an average of 5 hours and 20.99 minutes at work, which was significantly less than was found in a large sample of U.S. working adults. Firefighters endorsed poor sleep efficiency and poor sleep quality as assessed by self-report and objective actigraphy. One limitation is that only 12 of the 45 firefighters endorsed responding to a nighttime call during the work night that occurred between the two neuropsychological assessments. Comparing changes in neuropsychological performance between firefighters who had disrupted sleep compared to firefighters who did not, significant performances decrements were evident only for the psychomotor vigilance test (PVT) reaction time. If confirmed with a larger sample, the results suggest that reaction time may be a sensitive indicator of decreasing cognitive performance because of sleep loss. Limitations, future study directions, and recommendations are discussed.
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Date Issued
2018
Identifier
CFE0007251, ucf:52190
Format
Document (PDF)
PURL
http://purl.flvc.org/ucf/fd/CFE0007251
Sleep Disturbances Among Combat Military Veterans: A Comparative Study
Title
Sleep Disturbances Among Combat Military Veterans: A Comparative Study.
Creator
Stout, Jeremy, Beidel, Deborah, Neer, Sandra, Bowers, Clint, University of Central Florida
Abstract / Description
The sleep characteristics of 37 military combat veterans (17 with PTSD and 20 without PTSD) of recent wars were analyzed to determine if combat deployment, with its resultant wartime sleep restriction, may be an alternative explanation for the sleep duration complaints found among combat veterans with PTSD (as determined by PCL-M scores). Participants completed sleep actigraphy and a self-report measure of sleep duration over a 1-week period. Although strongly correlated, a comparison of...
Show moreThe sleep characteristics of 37 military combat veterans (17 with PTSD and 20 without PTSD) of recent wars were analyzed to determine if combat deployment, with its resultant wartime sleep restriction, may be an alternative explanation for the sleep duration complaints found among combat veterans with PTSD (as determined by PCL-M scores). Participants completed sleep actigraphy and a self-report measure of sleep duration over a 1-week period. Although strongly correlated, a comparison of subjective (e.g., self-report) and objective (e.g., actigraphy) sleep duration revealed a significant difference in sleep duration based on method of assessment. With respect to group differences, actigraphy data did not reveal a significant difference in sleep duration based on the presence of PTSD, whereas there was a significant difference based on self-report. Veterans without PTSD self- reported a significantly longer sleep duration than what was recorded by actigraphy; a difference of one hour and 11 minutes (p = 0.000); the difference was one hour and nine minutes. In an effort to determine why veterans without PTSD perceived that they slept longer than they actually did, factors related to sleep quality were examined. The results indicated that measures of hyper arousal (anger, anxiety, nightmares) were significantly correlated with sleep duration, suggesting a pattern of autonomic arousal that is known to interfere with restful sleep. Further research is necessary to determine if the sleep of veteran with PTSD is actually different from that of combat veterans without PTSD, and if such differences are actually amenable to treatment for PTSD.
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Date Issued
2015
Identifier
CFE0006232, ucf:51055
Format
Document (PDF)
PURL
http://purl.flvc.org/ucf/fd/CFE0006232