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Title: The generation of synthetic speech sounds by digital coding.
Creator: Steinberger, Eddy Alan, null, null, Engineering
Abstract / Description: FLorida Technological University College of Engineering Thesis; The feasibility of representing human speech by serial digital codes was investigated by exercising specially constructed digital logic coupled with standard audio output equipment. The theories being tested represent a radical departure from previous efforts in the field of speech research. Therefore, this initial investigation was limited in scope to a study of unconnected English language speech sounds at the phenome level.
Date Issued: 1975
Identifier: CFR0002823, ucf:52917
Format: Document (PDF)
PURL: http://purl.flvc.org/ucf/fd/CFR0002823

Title: Digital Image Processing by the Two-Dimensional Discrete Fourier Transform Method.
Creator: Joels, Lyman F., null, null, Engineering
Abstract / Description: Florida Technological University College of Engineering Thesis; The present study was conducted to ascertain undergraduate views about the effectiveness of International Teaching Assistants (ITAs) in the American classroom. The study was administered to a stratified cluster sampling by college of the target population, undergraduate students at the University of Central Florida, in Orlando, Florida. The instrument used, Questionnaire of Undergraduates about International Teaching Assistants ...
Show moreFlorida Technological University College of Engineering Thesis; The present study was conducted to ascertain undergraduate views about the effectiveness of International Teaching Assistants (ITAs) in the American classroom. The study was administered to a stratified cluster sampling by college of the target population, undergraduate students at the University of Central Florida, in Orlando, Florida. The instrument used, Questionnaire of Undergraduates about International Teaching Assistants (QUITA) as developed by Wanda Fox (1990), is composed of a total of 40 items regarding personal and academic background, cultural exposure to and views about non-native speakers of English, and ITA-classroom effectiveness and problem-solving strategies. On the basis of data from the Fall 1998 semester, approximately 15% of the total number of ITA-taught course sections per college were surveyed. The subjects responded anonymously using computerized answer sheets. Upon completion of the data collection phase, all surveys were analyzed for response frequencies. In addition, background and demographic information regarding the participants and information regarding undergraduate exposure to IT As and IT A instruction were also summarized. The Likert-type items were combined to reveal an overall ATITA (Attitude toward International Teaching Assistants) score. The results of the ATITA portion of the study indicate that undergraduate student views toward IT As and IT A instruction are between neutral and mildly positive. Furthermore, survey responses indicated that undergraduates resolve conflicts involving IT As through personal means. The closing recommendations suggest maintaining open lines of communication between undergraduates, ITAs, and administrators alike.
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Date Issued: 1973
Identifier: CFR0004782, ucf:52960
Format: Document (PDF)
PURL: http://purl.flvc.org/ucf/fd/CFR0004782

Title: Computer Simulation of 911 Emergency Telephone System for Orange County, Florida.
Creator: Veillette, Ronald J., null, null, Engineering
Abstract / Description: Florida Technological University College of Engineering Thesis; The State of Florida has mandated that a statewide 911 system be implemented, and the Department of General Services, Division of Communications, has performed a study of possible 911 system configurations for Orange County based on the findings of a study performed by the Stanford Research Institute for the whole of the State of Florida. The Orange County study determined operator manning levels of the primary law enforcement...
Show moreFlorida Technological University College of Engineering Thesis; The State of Florida has mandated that a statewide 911 system be implemented, and the Department of General Services, Division of Communications, has performed a study of possible 911 system configurations for Orange County based on the findings of a study performed by the Stanford Research Institute for the whole of the State of Florida. The Orange County study determined operator manning levels of the primary law enforcement agencies involved in the system and response times to citizen calls for each of the configurations proposed. This research generated computer simulation models of the two most likely to be implemented configurations for handling citizen calls. The models were run sing the input parameters defined in the Orange County study, and the results compared favorably. Additional runs were made with varying resource assignments to evaluate call service with respect to the level of performance and response time. The models were designed in modular form, such that they can easily be structured to conform to other operational configurations. This will allow evaluation of prosed systems prior to actual start up and analysis of resource requirements based on population predictions.
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Date Issued: 1977
Identifier: CFR0004777, ucf:52962
Format: Document (PDF)
PURL: http://purl.flvc.org/ucf/fd/CFR0004777

Title: Software quality assurance.
Creator: Soistman, Edward C., null, null, Engineering
Abstract / Description: University of Central Florida College of Engineering Thesis; The problems associated with software development and use are investigated from a management point of view. Having identified the critical aspects of effective software management, an approach is suggested for the creation and implementation of a software quality assurance program, Particular attention is focused on the concept of Life Cycle Procurement as currently utilized by the Department of Defense. The research was...
Show moreUniversity of Central Florida College of Engineering Thesis; The problems associated with software development and use are investigated from a management point of view. Having identified the critical aspects of effective software management, an approach is suggested for the creation and implementation of a software quality assurance program, Particular attention is focused on the concept of Life Cycle Procurement as currently utilized by the Department of Defense. The research was accomplished in two phases. The first consisted of an extensive literature search, seminar attendance and participation in several working groups assigned the responsibility for establishing software quality assurance guidelines. The second phase involved direct participation in the development of a formal software quality assurance program. The report is written in a manner designed to guide a non-technically oriented manager through a complete analysis of software, its measures of quality, its problem sources and the most promising techniques which can he used to control and evaluate its development.
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Date Issued: 1979
Identifier: CFR0003518, ucf:52989
Format: Document (PDF)
PURL: http://purl.flvc.org/ucf/fd/CFR0003518

Title: An immunological approach to the study of the tumorous-head trait in Drosophila melanogaster.
Creator: Weihe, Patricia Neuhaus, null, null, Social Sciences
Abstract / Description: Florida Technological University College of Natural Sciences Thesis;
Date Issued: 1975
Identifier: CFR0004337, ucf:52990
Format: Document (PDF)
PURL: http://purl.flvc.org/ucf/fd/CFR0004337

Title: The effects of enzymes on activated sludge floc.
Creator: Stoddart, Terry Lee, null, null, Natural Sciences
Abstract / Description: Florida Technological University College of Natural Sciences Thesis; Dialysed activated sludge was used as a substrate to test for enzymes which can hydrolyse activated sludge floc. Two hundred and fifty aerobic and anaerobic microorganisms were tested against activated sludge for the presence of hydrolytic enzymes. These included known genera and organisms obtained by various enrichment procedures. Anaerobic digester mixed liquor was contacted with activated sludge agar under anaerobic...
Show moreFlorida Technological University College of Natural Sciences Thesis; Dialysed activated sludge was used as a substrate to test for enzymes which can hydrolyse activated sludge floc. Two hundred and fifty aerobic and anaerobic microorganisms were tested against activated sludge for the presence of hydrolytic enzymes. These included known genera and organisms obtained by various enrichment procedures. Anaerobic digester mixed liquor was contacted with activated sludge agar under anaerobic conditions. None of the microorganisms present in the digester liquor hydrolysed the floc. The following commercial enzymes were contacted with activated sludge singly, in combination, and sequentially under various physical and chemical conditions: protease, lipase, cellulase, pectinase, phospholipase C, trypsin, and glucuronidase. Although commercial enzymes and various microorganisms reacted with known substrate controls neither the enzymes nor microorganisms employed affected the activated sludge floc. Treatment of activated sludge floc with ethylenediaminetetraacetic acid resulted in gross deflocculation and release of humic substances. The floc particle, probably a combination of polymers bound in a complex manner, is resistant to enzymatic degradation. Several workers have reported on the enzymatic hydrolysis of polymers produced by axenic cultures isolated from activated sludge floc. It is unlikely that the results of their work can be extrapolated to the complex structure of activated sludge floc particles.
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Date Issued: 1977
Identifier: CFR0003513, ucf:53004
Format: Document (PDF)
PURL: http://purl.flvc.org/ucf/fd/CFR0003513

Title: An empirical investigation into the determinants of the federal funds rate with special emphasis on trends in market participation.
Creator: Martin, Michael A., null, null, Business Administration
Abstract / Description: Florida Technological University College of Business Administration Thesis
Date Issued: 1978
Identifier: CFR0003523, ucf:53000
Format: Document (PDF)
PURL: http://purl.flvc.org/ucf/fd/CFR0003523

Title: Wastewater Treatment Plant Control.
Creator: Knowles, David M., null, null, Engineering
Abstract / Description: Florida Technological University College of Engineering Thesis
Date Issued: 1972
Identifier: CFR0003514, ucf:53001
Format: Document (PDF)
PURL: http://purl.flvc.org/ucf/fd/CFR0003514

Title: Triggered Sweep Generators Using Modern Integrated Circuits.
Creator: Klinger, Arthur Russell, null, null, Engineering
Abstract / Description: Florida Technological University College of Engineering Thesis; The oscilloscope is undoubtedly one of the most important tools in any electronics shop. Presently there are many relatively inexpensive oscilloscopes available, but triggered sweep capability is still reserved for those oscilloscopes costing more than 200 - 500 dollars. The goal of this project was to develop a respectably performing triggered sweep system at a low enough cost to allow inclusion of this valuable feature in any...
Show moreFlorida Technological University College of Engineering Thesis; The oscilloscope is undoubtedly one of the most important tools in any electronics shop. Presently there are many relatively inexpensive oscilloscopes available, but triggered sweep capability is still reserved for those oscilloscopes costing more than 200 - 500 dollars. The goal of this project was to develop a respectably performing triggered sweep system at a low enough cost to allow inclusion of this valuable feature in any oscilloscope. Two of the most important specifications of an oscilloscope are the bandwidth of the vertical amplifier, and the maximum sweep frequency. The broad class of "inexpensive oscilloscope" would include those with a maximum vertical response of 500 KHz to 5 MHz , and a maximum sweep rate of 50 KHz to 500 KHz . Most of these oscilloscopes would not have triggered sweep capability. For about double the cost, the next step upward would be a semi- professional triggered - sweep oscilloscope having a typical vertical response of 1511Hz and a sweep to roughly 2 MHz (500 nsec). Using these classifications as guidelines, a "respectably performing" triggered sweep for inexpensive oscilloscopes may be loosely defined as one having a 500 KHz (2 nsec) sweep, triggerable to at least 5 MHz. Depending on actual cost and application , greater or lesser performance could be considered entirely acceptable. A number of design variations are possible, all of which appear to be a fraction of the cost and complexity of previous designs having comparable specifications . Making this possible are integrated circuits in general, and a modern linear IC "timer" in particular. This report first describes this timer, then uses it as the main element in the generation of a linear ramp. Several trigger and gating circuits are then described. Finally, several of these subcircuits are combined to form three (out of many possible) complete triggered sweep generator systems. As an example of the results, the most expensive circuit costs about 15 dollars in single quantity, yet offers 10 volt per 200 nsec sweep rates, trigger capability from OC to above 15 MHz, trigger level and phase control, and blanking pulse output.
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Date Issued: 1973
Identifier: CFR0003511, ucf:53002
Format: Document (PDF)
PURL: http://purl.flvc.org/ucf/fd/CFR0003511

Title: The effects of a persuasive communication on the perception of a message film.
Creator: Fink, Marianell Branum, null, null, Social Sciences
Abstract / Description: Florida Technological University College of Social Sciences Thesis; The primary purpose of this study is to examine, in a controlled experimental situation, two variables that might have an effect upon the viewer's perception of a message film. One variable that can be expected to affect perception is the viewer's prior knowledge of the film. This study deals with one specific variable - a published review. Another variable that might affect perception is personality. This study deals with...
Show moreFlorida Technological University College of Social Sciences Thesis; The primary purpose of this study is to examine, in a controlled experimental situation, two variables that might have an effect upon the viewer's perception of a message film. One variable that can be expected to affect perception is the viewer's prior knowledge of the film. This study deals with one specific variable - a published review. Another variable that might affect perception is personality. This study deals with one identified personality type - authoritarian. The message film is the award-winning, five-minute, color, anti-war film, Star Spangled Banner. It was produced and directed by Roger Flint and was released in 1972. The sections that follow examine a variety of previous research findings, develop the theoretical concepts, report the method of study, and analyze the findings.
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Date Issued: 1976
Identifier: CFR0004328, ucf:52997
Format: Document (PDF)
PURL: http://purl.flvc.org/ucf/fd/CFR0004328

Title: Reproductive parameters for nine avian species at Moore Creek, Merritt Island National Wildlife Refuge.
Creator: Girard, G. Tanner, null, null, Natural Sciences
Abstract / Description: Florida Technological University College of Natural Sciences Thesis;
Date Issued: 1976
Identifier: CFR0004769, ucf:52980
Format: Document (PDF)
PURL: http://purl.flvc.org/ucf/fd/CFR0004769

Title: Feminine sex roles and depression in middle-aged women.
Creator: Tinsley, Emily G., null, null, Social Sciences
Abstract / Description: University of Central Florida College of Social Sciences Thesis; The preponderance of females in the depressed population is a well established fact. Various hypotheses for this fact are reviewed and the hypothesis that females accepting the feminine role will be more likely to become depressed during their middle years than a more androgenous woman is submitted. To support this hypothesis a study was designed utilizing a Clinical group consisting of females, 35 to 50 experiencing depression...
Show moreUniversity of Central Florida College of Social Sciences Thesis; The preponderance of females in the depressed population is a well established fact. Various hypotheses for this fact are reviewed and the hypothesis that females accepting the feminine role will be more likely to become depressed during their middle years than a more androgenous woman is submitted. To support this hypothesis a study was designed utilizing a Clinical group consisting of females, 35 to 50 experiencing depression and undergoing treatment for depression at one of three mental health centers and a Non-clinical group consisting of women, same age group, not experiencing depression and who had never undergone treatment for depression. Each subject was asked to complete a questionnaire to measure depression and femininity. Results of this study support the hypothesis that depression in middle-aged females is related to the degree of their acceptance of the traditional feminine role. Depression as defined and measured by Beck's Depression Inventory was positively correlated with femininity as measured by Bern's Sex-Role Inventory. The Pearson correlation coefficient for these two measures was .62 (p<.001), indicating that 38% of the variance in depression scores can be accounted for by the sex-role variable.
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Date Issued: 1979
Identifier: CFR0004425, ucf:52981
Format: Document (PDF)
PURL: http://purl.flvc.org/ucf/fd/CFR0004425

Title: A serological survey of greater Orlando human, canine, and feline populations for the presence of antibodies to Toxoplasma gondii.
Creator: Weihe, John Louis, null, null, Natural Sciences
Abstract / Description: Florida Technological University College of Natural Sciences Thesis; Toxoplasma gondii, the etiological agent of toxoplasmosis, was first discovered in Ctenodactylus gondi, an small North African rodent, by Nicolle and Manceaux in 1908. Later the same year Splendore observed the organism in a laboratory rabbit. Human infection was first reported by Janku in 1923. Since its discovery, many investigations have shown the organism to be truly unique in the protozoan world. There appears to be...
Show moreFlorida Technological University College of Natural Sciences Thesis; Toxoplasma gondii, the etiological agent of toxoplasmosis, was first discovered in Ctenodactylus gondi, an small North African rodent, by Nicolle and Manceaux in 1908. Later the same year Splendore observed the organism in a laboratory rabbit. Human infection was first reported by Janku in 1923. Since its discovery, many investigations have shown the organism to be truly unique in the protozoan world. There appears to be only one species and only one serotype. Serologic and microscopic studies reveal Toxoplasma to have a vast host range. At least one representative of virtually every mammalian species tested harbored the parasite or showed the presence of demonstrable antibody. Studies of toxoplasmal infection among mammalian populations show that the prevalence of infection usually increases: 1 ) in warm, moist climates and 2) with age. Sex, however, appears to have little influence on the prevalence of the parasite. One most unusual feature of the organism is its lack of target cell specificity: Toxoplasma is an obligate, intracellular parasite capable of living in any cell except non-nucleated erythrocytes. Infection by Toxoplasma is most often confirmed serologically. Many procedures have been described; however, the Sabin-Feldman dye test, the indirect hemagglutinstion test, and the indirect fluorescent antibody test appear to be superior methods for antibody detection. The indirect fluorescent antibody test was selected for this study because: 1) it does not require the use of live organisms, 2) it is relatively easy to perform, and 3) it correlates well with the standard Sabin-Feldman dye test. This study was undertaken to determine the role of various factors on toxoplasmal prevalence in the Greater Orlando area, Age, sex, and race were considered for each human sample tested. Age, sex, and relative degree of outdoor exposure were considered in the study of each canine and feline sample.
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Date Issued: 1975
Identifier: CFR0004336, ucf:52992
Format: Document (PDF)
PURL: http://purl.flvc.org/ucf/fd/CFR0004336

Title: The effects of altered traffic signs upon vehicular driving modes and consequent fuel conservation and environmental benefits, as measured by vehicular noise-imprints.
Creator: Pfarrer, Mark Daniel, null, null, Engineering
Abstract / Description: Florida Technological University College of Engineering Thesis; The hypothesis is that the recorded noise-imprints of a vehicle at an intersection can be used to identify and accurately time the driving modes of deceleration, idle, slow cruise, and acceleration. This is proven by analyzing and comparing noise-imprints of vehicles at an uncontrolled intersection marked first with a 'stop' sign, and then by a 'yield' and an experimental 'dead slow' sign. By relating the duration of each driving...
Show moreFlorida Technological University College of Engineering Thesis; The hypothesis is that the recorded noise-imprints of a vehicle at an intersection can be used to identify and accurately time the driving modes of deceleration, idle, slow cruise, and acceleration. This is proven by analyzing and comparing noise-imprints of vehicles at an uncontrolled intersection marked first with a 'stop' sign, and then by a 'yield' and an experimental 'dead slow' sign. By relating the duration of each driving mode to known relations, the overall efficiency of an intersection can be characterized. A new technique for studying various types of traffic conditions at intersections is the result. Initial noise-imprint analysis and comparison shows that a 'yield' sign is to be preferred over a 'stop' sign to decrease travel time, air pollution emissions, gasoline consumption, and wear-and-tear on the car. The experimental 'dead slow' sign is used as a demonstration of the noise-imprint technique upon an unknown situation. The efficiency of a 'dead slow' sign proved to be less than that of a 'yield' sign, but still greater than that of a 'stop' sign.
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Date Issued: 1976
Identifier: CFR0003525, ucf:52986
Format: Document (PDF)
PURL: http://purl.flvc.org/ucf/fd/CFR0003525

Title: The Design of a Digital Data Acquisition System for Jet Engine Testing.
Creator: Carter, Robert W., null, null, Engineering
Abstract / Description: Florida Technological University College of Engineering Thesis; This research report documents the various types of Data Acquisition Systems in use for testing jet aircraft engines. The cost trade offs and design considerations are explored for systems which employ a digital computer as the prime recording/processing element. The digital computer has revolutionalized the data acquisition field, particularly in the testing of high performance jet engines. Test data can be acquired, processed,...
Show moreFlorida Technological University College of Engineering Thesis; This research report documents the various types of Data Acquisition Systems in use for testing jet aircraft engines. The cost trade offs and design considerations are explored for systems which employ a digital computer as the prime recording/processing element. The digital computer has revolutionalized the data acquisition field, particularly in the testing of high performance jet engines. Test data can be acquired, processed, converted to engineering units, and out via high speed line printers and cathode ray tubes (CRT's). The data acquisition system operates on-line, and interleaves the random requests for data from multiple test cells by using a specially designed software system and multi-processing capability of the high speed digital computer. All test data must be traceable to The National Bureau of Standards, which required that all calibration standards also be traceable. Primary and secondary calibration methods are discussed and examples of the mathematical processes for conversion of the raw data to meaningful results are presented. Data Acquisition Systems for jet engine testing can be logically grouped into two main categories, with the determining factor being the type of test to be conducted. Production engine testing requires rapid setup, calibration, and fast data turn around, particularly for modern automated test facilities. Development engine testing requires a large number of data channels, infrequent setup, and complete software for extensive engine performance calculations. Both types of Data Acquistion Systems have been designed and built by Pratt and Whitney Aircraft and are used as examples of the techniques described in this report.
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Date Issued: 1973
Identifier: CFR0004770, ucf:52982
Format: Document (PDF)
PURL: http://purl.flvc.org/ucf/fd/CFR0004770

Title: System safety in rail transportation.
Creator: Smith, Quave Norvell, null, null, Engineering
Abstract / Description: Florida Technological University College of Engineering Thesis; This thesis, "System Safety in Rail Transportation" is addressed to an individual having a basic technical background but little or no experience in this field. The thesis discusses the need for and the benefits to be obtained by using system safety techniques and principles in the railroad industry. Examples of typical railroad accidents are reviewed, and it is pointed out that analysis of the hazards in the railroad industry...
Show moreFlorida Technological University College of Engineering Thesis; This thesis, "System Safety in Rail Transportation" is addressed to an individual having a basic technical background but little or no experience in this field. The thesis discusses the need for and the benefits to be obtained by using system safety techniques and principles in the railroad industry. Examples of typical railroad accidents are reviewed, and it is pointed out that analysis of the hazards in the railroad industry prior to the accidents would have identified problems which eventually resulted in the accidents. The system safety approach, which was developed for use in the aerospace and aviation fields, has proved to be extremely effective and is now being adapted to many other areas. The surface modes of transportation have the greatest need for these techniques. The techniques covered in this thesis include Hazard Analysis, Fault or Logic Tree Analysis, Failure Modes and Effects Analysis, and Probabilistic Cost Analysis. The thesis also describes a hypothetical model for organizing and implementing system safety approaches in an existing railroad company.
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Date Issued: 1976
Identifier: CFR0003512, ucf:53012
Format: Document (PDF)
PURL: http://purl.flvc.org/ucf/fd/CFR0003512

Title: A survey of weapon system cost effectiveness methodologies.
Creator: Fritz, John T., null, null, Engineering
Abstract / Description: Florida Technological University College of Engineering Thesis; A survey of cost effectiveness methodologies used in the defense industry is presented and an application of cost effectiveness is developed. A breakdown in the level of the decision making is made and follows the example of the Weapon System Effectiveness Industry Advisory Committee. Examples of cost effectiveness methodologies at each decision making level are shown.
Date Issued: 1976
Identifier: CFR0003499, ucf:53008
Format: Document (PDF)
PURL: http://purl.flvc.org/ucf/fd/CFR0003499

Title: Simplification of underwater acoustic equations.
Creator: Middleton, Morris G., null, null, Engineering
Abstract / Description: Florida Technological University College of Engineering Thesis; The equations of underwater acoustics that relate to the signal excess noise received by a transducer are rigorously described in the acoustical literature. The objective of this paper is to examine some underwater acoustic equations and ascertain if a simplification can be obtained without affecting the overall results. The literature that is available on underwater acoustics would fill a university library and is growing each...
Show moreFlorida Technological University College of Engineering Thesis; The equations of underwater acoustics that relate to the signal excess noise received by a transducer are rigorously described in the acoustical literature. The objective of this paper is to examine some underwater acoustic equations and ascertain if a simplification can be obtained without affecting the overall results. The literature that is available on underwater acoustics would fill a university library and is growing each day. Thus, this paper addresses some of the factors that contribute to the signal excess noise equation and is by no means a complete treatment of the subject.
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Date Issued: 1973
Identifier: CFR0003480, ucf:53022
Format: Document (PDF)
PURL: http://purl.flvc.org/ucf/fd/CFR0003480

Title: Application of system dynamics techniques to war game modeling.
Creator: Amico, G. Vincent, null, null, Engineering
Abstract / Description: Florida Technological University College of Engineering Thesis; Military War Gaming has developed from- the chess-like games to the elaborate computer simulations of today, using high-speed, general-purpose, digital computers. This Research Report will briefly review modern war-game developments with emphasis on real-t h e training systems. The application of system dynamics techniques developed by Jay W. Forrester to war-game modeling is explored. A simple destroyer versus submarine model is...
Show moreFlorida Technological University College of Engineering Thesis; Military War Gaming has developed from- the chess-like games to the elaborate computer simulations of today, using high-speed, general-purpose, digital computers. This Research Report will briefly review modern war-game developments with emphasis on real-t h e training systems. The application of system dynamics techniques developed by Jay W. Forrester to war-game modeling is explored. A simple destroyer versus submarine model is developed. Results indicate that the system dynamics modeling technique is a powerful and effective tool. However, the Dynamo language could be substantially improved by a more powerful logical statement capability.
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Date Issued: 1973
Identifier: CFR0003482, ucf:53032
Format: Document (PDF)
PURL: http://purl.flvc.org/ucf/fd/CFR0003482

Title: The feasibility of detecting poliovirus I in water by radioimmunoassay.
Creator: Charba, Sheril K., null, null, Natural Sciences
Abstract / Description: University of Central Florida College of Natural Sciences Thesis; A microtiter, solid-phase, indirect radioimmunoassay (RIA) has been developed and evaluated as a method for detecting poliovirus in water samples. Antiserum to poliovirus Type I, LSc2ab, was prepared in rabbits, and high titer, high avidity antiserum to rabbit globulins were radio-125 actively labeled with I by a modification of the Bolton-Hunter and chloramine T methods to increasing specific activities. The immunoreactivity...
Show moreUniversity of Central Florida College of Natural Sciences Thesis; A microtiter, solid-phase, indirect radioimmunoassay (RIA) has been developed and evaluated as a method for detecting poliovirus in water samples. Antiserum to poliovirus Type I, LSc2ab, was prepared in rabbits, and high titer, high avidity antiserum to rabbit globulins were radio-125 actively labeled with I by a modification of the Bolton-Hunter and chloramine T methods to increasing specific activities. The immunoreactivity of the labeled antibodies was assessed. After the preparation and standardization of all components of the assay. The optimum assay conditions were determined. These conditions included the method of coating the microtiter wells with antigen, the time and temperature of incubation of each antiserum, the dilution of each antiserum, and means of reducing non-specific binding. The RIA was conducted with varying numbers of viral plaque forming units. In replicate experiments, average binding ratios of 784% were obtained when different numbers of virus were first reacted with antiserum. This technique shows increased sensitivity over the direct method. These results indicate that the use of RIA to detect viruses in water is indeed feasible.
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Date Issued: 1999
Identifier: CFR0003478, ucf:53030
Format: Document (PDF)
PURL: http://purl.flvc.org/ucf/fd/CFR0003478

Adolescent psychology (1)	+ -
Airplanes -- Jet propulsion (1)	+ -
Airplanes -- Motors -- Testing (1)	+ -
Airplanes -- Motors -- Testing -- Data processing (1)	+ -
Automobiles -- Environmental aspects (1)	+ -

Birds (1)	+ -
Computer programming (1)	+ -
Computer programs -- Reliability (1)	+ -
Cost effectiveness (1)	+ -
Depression (1)	+ -
Drosophila melanogaster (1)	+ -
Electric (1)	+ -
Emergency communication systems (1)	+ -
Emergency communication systems -- Data processing (1)	+ -
Federal Reserve bank (1)	+ -
Federal funds market (United States) (1)	+ -
Femininity (1)	+ -
Fourier transformations (1)	+ -
Integrated circuits (1)	+ -
Mental (1)	+ -
Military art and science -- Data processing (1)	+ -
Optical data processing (1)	+ -
Oscillators (1)	+ -
Perception (1)	+ -
Persuasion (Psychology) (1)	+ -
Poliovirus (1)	+ -
Radioimmunoassay (1)	+ -
Railroads -- Safety measures (1)	+ -
Reproduction (1)	+ -
Resorts -- Florida (1)	+ -

null, null (22)	+ -
Engineering (13)	+ -
Natural Sciences (4)	+ -
Social Sciences (4)	+ -
Amico, G. Vincent (1)	+ -

Barraclough, Bradley (1)	+ -
Business Administration (1)	+ -
Carter, Robert W. (1)	+ -
Charba, Sheril K. (1)	+ -
Fink, Marianell Branum (1)	+ -
Fritz, John T. (1)	+ -
Girard, G. Tanner (1)	+ -
Joels, Lyman F. (1)	+ -
Kinnaird, Mary Ellen Moore (1)	+ -
Klinger, Arthur Russell (1)	+ -
Knowles, David M. (1)	+ -
Martin, Michael A. (1)	+ -
Middleton, Morris G. (1)	+ -
Pfarrer, Mark Daniel (1)	+ -
Smith, Quave Norvell (1)	+ -
Soistman, Edward C. (1)	+ -
Steinberger, Eddy Alan (1)	+ -
Stoddart, Terry Lee (1)	+ -
Tinsley, Emily G. (1)	+ -
Veillette, Ronald J. (1)	+ -
Weihe, John Louis (1)	+ -
Weihe, Patricia Neuhaus (1)	+ -

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