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(161 - 171 of 171)
Pages
- Title
- Development of human and rodent based in vitro systems toward better translation of bench to bedside in vivo results.
- Creator
-
Berry, Bonnie, Hickman, James, Khaled, Annette, Lambert, Stephen, Sugaya, Kiminobu, University of Central Florida
- Abstract / Description
-
Prospective medicinal compounds progress through multiple testing phases before becoming licensed drugs. Testing of novel compounds includes a preclinical phase where the potential therapeutic is tested in vitro and/or in animal models in vivo to predict its potential efficacy and/or toxicity in humans. The failure of preclinical models to accurately predict human drug responses can lead to potentially dangerous compounds being administered to humans, or potentially beneficial compounds being...
Show moreProspective medicinal compounds progress through multiple testing phases before becoming licensed drugs. Testing of novel compounds includes a preclinical phase where the potential therapeutic is tested in vitro and/or in animal models in vivo to predict its potential efficacy and/or toxicity in humans. The failure of preclinical models to accurately predict human drug responses can lead to potentially dangerous compounds being administered to humans, or potentially beneficial compounds being kept in development abeyance. Moreover, inappropriate choice in model organism for studying disease states may result in pushing forward inappropriate drug targets and/or compounds and wasting valuable time and resources in producing much-needed medications. In this dissertation, models for basic science research and drug testing are investigated with the intention of improving current preclinical models in order to drive drugs to market faster and more efficiently. We found that embryonic rat hippocampal neurons, commonly used to study neurodegenerative disease mechanisms in vitro, take 3-4 weeks to achieve similar, critical ion-channel expression profiles as seen in adult rat hippocampal cultures. We also characterized a newly-available commercial cell line of human induced pluripotent stem cell-derived neurons for their applicability in long-term studies, and used them to develop a more pathologically relevant model of early Alzheimer's Disease in vitro. Finally, we attempted to create an engineered, layered neural network of human neurons to study drug responses and synaptic mechanisms. Utilization of the results and methods described herein will help push forward the development of better model systems for translation of laboratory research to successful clinical human drug trials.
Show less - Date Issued
- 2015
- Identifier
- CFE0006261, ucf:51031
- Format
- Document (PDF)
- PURL
- http://purl.flvc.org/ucf/fd/CFE0006261
- Title
- Civil government in Florida under state and federal constitutions.
- Creator
-
Yocum, Wilbur Fisk, PALMM (Project)
- Abstract / Description
-
Examines and explains the articles and sections of the Constitution of the State of Florida for the purpose of providing instruction on civil government. This is followed by a briefer examination of the Constitution of the United States. Each part includes a "Topical analysis" which arranges the provisions of the constitutions according to subject matter.
- Date Issued
- 1905
- Identifier
- AAA6250QF00004/30/200308/12/200320964BfamIa D0QF, ONICF169- 0, FCLA url 20030810xOCLC, 52834001, CF00001607, 2569630, ucf:12770
- Format
- E-book
- PURL
- http://purl.flvc.org/fcla/tc/fhp/CF00001607.jpg
- Title
- Chemometric Applications to a Complex Classification Problem: Forensic Fire Debris Analysis.
- Creator
-
Waddell, Erin, Sigman, Michael, Belfield, Kevin, Campiglia, Andres, Yestrebsky, Cherie, Ni, Liqiang, University of Central Florida
- Abstract / Description
-
Fire debris analysis currently relies on visual pattern recognition of the total ion chromatograms, extracted ion profiles, and target compound chromatograms to identify the presence of an ignitable liquid according to the ASTM International E1618-10 standard method. For large data sets, this methodology can be time consuming and is a subjective method, the accuracy of which is dependent upon the skill and experience of the analyst. This research aimed to develop an automated classification...
Show moreFire debris analysis currently relies on visual pattern recognition of the total ion chromatograms, extracted ion profiles, and target compound chromatograms to identify the presence of an ignitable liquid according to the ASTM International E1618-10 standard method. For large data sets, this methodology can be time consuming and is a subjective method, the accuracy of which is dependent upon the skill and experience of the analyst. This research aimed to develop an automated classification method for large data sets and investigated the use of the total ion spectrum (TIS). The TIS is calculated by taking an average mass spectrum across the entire chromatographic range and has been shown to contain sufficient information content for the identification of ignitable liquids. The TIS of ignitable liquids and substrates, defined as common building materials and household furnishings, were compiled into model data sets. Cross-validation (CV) and fire debris samples, obtained from laboratory-scale and large-scale burns, were used to test the models. An automated classification method was developed using computational software, written in-house, that considers a multi-step classification scheme to detect ignitable liquid residues in fire debris samples and assign these to the classes defined in ASTM E1618-10. Classifications were made using linear discriminant analysis, quadratic discriminant analysis (QDA), and soft independent modeling of class analogy (SIMCA). Overall, the highest correct classification rates were achieved using QDA for the first step of the scheme and SIMCA for the remaining steps. In the first step of the classification scheme, correct classification rates of 95.3% and 89.2% were obtained for the CV test set and fire debris samples, respectively. Correct classifications rates of 100% were achieved for both data sets in the majority of the remaining steps which used SIMCA for classification. In this research, the first statistically valid error rates for fire debris analysis have been developed through cross-validation of large data sets. The error rates reduce the subjectivity associated with the current methods and provide a level of confidence in sample classification that does not currently exist in forensic fire debris analysis.
Show less - Date Issued
- 2013
- Identifier
- CFE0004954, ucf:49586
- Format
- Document (PDF)
- PURL
- http://purl.flvc.org/ucf/fd/CFE0004954
- Title
- STATISTICAL ANALYSIS OF VISIBLE ABSORPTION SPECTRA AND MASS SPECTRA OBTAINED FROM DYED TEXTILE FIBERS.
- Creator
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White, Katie, Sigman, Michael, University of Central Florida
- Abstract / Description
-
The National Academy of Sciences recently published a report which calls for improvements to the field of forensic science. Their report criticized many forensic disciplines for failure to establish rigorously-tested methods of comparison, and encouraged more research in these areas to establish limitations and assess error rates. This study applies chemometric and statistical methods to current and developing analytical techniques in fiber analysis. In addition to analysis of commercially...
Show moreThe National Academy of Sciences recently published a report which calls for improvements to the field of forensic science. Their report criticized many forensic disciplines for failure to establish rigorously-tested methods of comparison, and encouraged more research in these areas to establish limitations and assess error rates. This study applies chemometric and statistical methods to current and developing analytical techniques in fiber analysis. In addition to analysis of commercially available dyed textile fibers, two pairs of dyes are selected for custom fabric dyeing based on the similarities of their absorbance spectra and dye molecular structures. Visible absorption spectra for all fiber samples are collected using microspectrophotometry (MSP) and mass spectra are collected using electrospray ionization (ESI) mass spectrometry. Statistical calculations are performed using commercial software packages and software written in-house. Levels of Type I and Type II error are examined for fiber discrimination based on hypothesis testing of visible absorbance spectra profiles using a nonparametric permutation method. This work also explores evaluation of known and questioned fiber populations based on an assessment of statistical p-value distributions from questioned-known fiber comparisons with those of known fiber self-comparisons. Results from the hypothesis testing are compared with principal components analysis (PCA) and discriminant analysis (DA) of visible absorption spectra, as well as PCA and DA of ESI mass spectra. The sensitivity of a statistical approach will also be discussed in terms of how instrumental parameters and sampling methods may influence error rates.
Show less - Date Issued
- 2010
- Identifier
- CFE0003454, ucf:48396
- Format
- Document (PDF)
- PURL
- http://purl.flvc.org/ucf/fd/CFE0003454
- Title
- Catalytically Enhanced Heterogeneous Combustion of methane.
- Creator
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Terracciano, Anthony, Orlovskaya, Nina, Vasu Sumathi, Subith, Chow, Louis, Kassab, Alain, University of Central Florida
- Abstract / Description
-
Heterogeneous combustion is an advanced internal combustion technique, which enables heat recuperation within the flame by utilizing a highly porous ceramic media as a regenerator. Heat released within the gas phase convectively transfers to the solid media. This heat within the solid media then travels towards the inlet, enabling reactant preheating. Such heat redistribution enables stable burning of both ultra-lean fuel/air mixtures, forming a more diffuse flame through the combustion...
Show moreHeterogeneous combustion is an advanced internal combustion technique, which enables heat recuperation within the flame by utilizing a highly porous ceramic media as a regenerator. Heat released within the gas phase convectively transfers to the solid media. This heat within the solid media then travels towards the inlet, enabling reactant preheating. Such heat redistribution enables stable burning of both ultra-lean fuel/air mixtures, forming a more diffuse flame through the combustion chamber, and results in reduced pollutant formation. To further enhance heterogeneous combustion, the ceramic media can be coated with catalytically active materials, which facilitates surface based chemical reactions that could occur in parallel with gas phase reactions.Within this work, a flow stabilized heterogeneous combustor was designed and developed consisting of a reactant delivery nozzle, combustion chamber, and external instrumentation. The reactant delivery nozzle enables the combustor to operate on mixtures of air, liquid fuel, and gaseous fuel. Although this combustor has high fuel flexibility, only gaseous methane was used within the presented experiments. Within the reactant delivery nozzle, reactants flow through a tube mixer, and a homogeneous gaseous mixture is delivered to the combustion chamber. ?-alumina (?-Al2O3), magnesia stabilized zirconia (MgO-ZrO2), or silicon carbide (SiC) was used as the material for the porous media. Measurement techniques which were incorporated in the combustor include an array of axially mounted thermocouples, an external microphone, an external CCD camera, and a gas chromatograph with thermal conductivity detector which enable temperature measurements, acoustic spectroscopy, characterization of thermal radiative emissions, and composition analysis of exhaust gasses, respectively. Before evaluation of the various solid media in the combustion chamber the substrates and catalysts were characterized using X-ray diffraction, X-ray fluorescence, scanning electron microscopy and energy dispersive spectroscopy. MgO-ZrO2 porous media was found to outperform both ?-Al2O3 and SiC matrices, as it was established that higher temperatures for a given equivalence ratio were achieved when the flame was contained within a MgO-ZrO2 matrix. This was explained by the presence of oxygen vacancies within the MgO doped ZrO2 fluorite lattice which facilitated catalytic reactions. Several catalyst compositions were evaluated to promote combustion within a MgO-ZrO2 matrix even further.Catalysts such as: Pd enhanced WC, ZrB2, Ce0.80Gd0.20O1.90, LaCoO3, La0.80Ca0.20CoO3, La0.75Sr0.25Fe0.95Ru0.05O3, and La0.75Sr0.25Cr0.95Ru0.05O3; were evaluated under lean fuel/air mixtures. LaCoO3 outperformed all other catalysts, by enabling the highest temperatures within the combustion chamber, followed by Ce0.80Gd0.20O1.90. Both LaCoO3 and Ce0.80Gd0.20O1.90 enabled a flame to exist at ?=0.45(&)#177;0.02, however LaCoO3 caused the flame to be much more stable. Furthermore, it was discovered that the coating of MgO-ZrO2 with LaCoO3 significantly enhanced the total emissive power of the combustion chamber. In this work as acoustic spectroscopy was used to characterize heterogeneous combustion for the first time. It was found that there is a dependence of acoustic emission n the equivalence ratio and flame position regardless of media and catalyst combination. It was also found that when different catalysts were used, the acoustic tones produced during combustion at fixed reactant flow rates were distinct
Show less - Date Issued
- 2016
- Identifier
- CFE0006508, ucf:51364
- Format
- Document (PDF)
- PURL
- http://purl.flvc.org/ucf/fd/CFE0006508
- Title
- Virtual Coaching of Novice Science Educators to Support Students with Emotional and Behavioral Disorders.
- Creator
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Garland, Dennis, Dieker, Lisa, Vasquez, Eleazar, Hines, Rebecca, Rosenberg, Michael, University of Central Florida
- Abstract / Description
-
Due to a multitude of convergent circumstances, students labeled in the disability category of emotional and behavioral disorders (EBD) experience high rates of academic and behavioral failure. Such failure frequently leads to the students' dropping out of school, involvement in the judicial system, or a combination of those outcomes. Science is an academic content area that has the potential to enhance behavioral and academic success of students with EBD. Researchers, nonprofits, and...
Show moreDue to a multitude of convergent circumstances, students labeled in the disability category of emotional and behavioral disorders (EBD) experience high rates of academic and behavioral failure. Such failure frequently leads to the students' dropping out of school, involvement in the judicial system, or a combination of those outcomes. Science is an academic content area that has the potential to enhance behavioral and academic success of students with EBD. Researchers, nonprofits, and business leaders have provided an impetus for nationwide reform in science education. Concurrently, a corpus of legislation has influenced the preparation of new teachers to use evidence-based teaching practices while addressing the needs of an increasingly diverse student population. Using technology is one way that teacher educators are providing in-vivo learning experiences to new teachers during their classroom instruction. A multiple-baseline across-participants research study was used to examine the effectiveness of providing immediate feedback (within three seconds) to novice general science educators to increase their use of an evidence-based teaching strategy, known as a three-term contingency (TTC) trial while they taught. Feedback was delivered via Bug-in-the-Ear (BIE) technology and during whole-class instruction in which students with EBD were included. The teacher participants wore a Bluetooth earpiece, which served as a vehicle for audio communication with the investigator. Teachers were observed via web camera over the Adobe(&)#174;ConnectTM online conferencing platform. During the intervention, teachers increased their percentage of completed TTC trials, opportunities to respond, and praise or error correction. Student responses also increased, and maladaptive behaviors decreased.
Show less - Date Issued
- 2013
- Identifier
- CFE0004847, ucf:49681
- Format
- Document (PDF)
- PURL
- http://purl.flvc.org/ucf/fd/CFE0004847
- Title
- Engineering and Application of Ultrafast Laser Pulses and Filamentation in Air.
- Creator
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Barbieri, Nicholas, Richardson, Martin, University of Central Florida
- Abstract / Description
-
Continuing advances in laser and photonic technology has seen the development of lasers with increasing power and increasingly short pulsewidths, which have become available over an increasing range of wavelengths. As the availability of laser sources grow, so do their applications. To make better use of this improving technology, understanding and controlling laser propagation in free space is critical, as is understanding the interaction between laser light and matter.The need to better...
Show moreContinuing advances in laser and photonic technology has seen the development of lasers with increasing power and increasingly short pulsewidths, which have become available over an increasing range of wavelengths. As the availability of laser sources grow, so do their applications. To make better use of this improving technology, understanding and controlling laser propagation in free space is critical, as is understanding the interaction between laser light and matter.The need to better control the light obtained from increasingly advanced laser sources leads to the emergence of beam engineering, the systematic understanding and control of light through refractive media and free space. Beam engineering enables control over the beam shape, energy and spectral composition during propagation, which can be achieved through a variety of means. In this dissertation, several methods of beam engineering are investigated. These methods enable improved control over the shape and propagation of laser light. Laser-matter interaction is also investigated, as it provides both a means to control the propagation of pulsed laser light through the atmosphere, and provides a means to generation remote sources of radiation.
Show less - Date Issued
- 2013
- Identifier
- CFE0004650, ucf:49881
- Format
- Document (PDF)
- PURL
- http://purl.flvc.org/ucf/fd/CFE0004650
- Title
- A history of Florida.
- Creator
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Brevard, Caroline Mays, Bennett, Henry Eastman, PALMM (Project)
- Abstract / Description
-
Parts I and II cover the history of Florida including its discovery by Ponce de Leon, the establishment of colonies by various European nations, its purchase by the United States, statehood, involvement in the Civil War, Reconstruction and brief descriptions of events through World War I. Part III describes the Internal Improvement Fund, Florida's school system and the civil government of Florida.
- Date Issued
- 1919
- Identifier
- AAA6239QF00004/30/200304/06/200516126BfamI D0QF, ONICF160- 7, FHP C CF 2003-04-30, FCLA url 20040208xOCLC, 58809397, CF00001590, 2567301, ucf:13153
- Format
- E-book
- PURL
- http://purl.flvc.org/fcla/tc/fhp/CF00001590.jpg
- Title
- THE KIOSK CULTURE: RECONCILING THE PERFORMANCE SUPPORT PARADOX IN THE POSTMODERN AGE OF MACHINES.
- Creator
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Cavanagh, Thomas, Kitalong, Karla, University of Central Florida
- Abstract / Description
-
Do you remember the first time you used an Automatic Teller Machine (ATM)? Or a pay-at-the-pump gas station? Or an airline e-ticket kiosk? How did you know what to do? Although you never received any formal instruction in how to interact with the self-service technology, you were likely able to accomplish your task (e.g., withdrawing or depositing money) as successfully as an experienced user. However, not so long ago, to accomplish that same task, you needed the direct mediation of a service...
Show moreDo you remember the first time you used an Automatic Teller Machine (ATM)? Or a pay-at-the-pump gas station? Or an airline e-ticket kiosk? How did you know what to do? Although you never received any formal instruction in how to interact with the self-service technology, you were likely able to accomplish your task (e.g., withdrawing or depositing money) as successfully as an experienced user. However, not so long ago, to accomplish that same task, you needed the direct mediation of a service professional who had been trained how to use the required complex technology. What has changed? In short, the technology is now able to compensate for the average consumer's lack of experience with the transactional system. The technology itself bridges the performance gap, allowing a novice to accomplish the same task as an experienced professional. This shift to a self-service paradigm is completely changing the dynamics of the consumer relationship with the capitalist enterprise, resulting in what is rapidly becoming the default consumer interface of the postmodern era. The recognition that the entire performance support apparatus now revolves around the end user/consumer rather than the employee represents a tectonic shift in the workforce training industry. What emerges is a homogenized consumer culture enabled by self-service technologies--a kiosk culture. No longer is the ability to interact with complex technology confined to a privileged workforce minority who has access to expensive and time-consuming training. The growth of the kiosk culture is being driven equally by business financial pressures, consumer demand for more efficient transactions, and the improved sophistication of compensatory technology that allows a novice to perform a task with the same competence as an expert. "The Kiosk Culture" examines all aspects of self-service technology and its ascendancy. Beyond the milieu of business, the kiosk culture is also infiltrating all corners of society, including medicine, athletics, and the arts, forcing us to re-examine our definitions of knowledge, skills, performance, and even humanity. The current ubiquity of self-service technology has already impacted our society and will continue to do so as we ride the rising tide of the kiosk culture.
Show less - Date Issued
- 2006
- Identifier
- CFE0001348, ucf:46989
- Format
- Document (PDF)
- PURL
- http://purl.flvc.org/ucf/fd/CFE0001348
- Title
- CLAIMS OF MISTAKEN IDENTITY:AN EXAMINATION OF U.S. TELEVISION FOOD COMMERCIALS AND THE ADULT OBESITY ISSUE.
- Creator
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Delgado, Cristina, DeLorme, Denise, University of Central Florida
- Abstract / Description
-
Obesity is one of the major public health issues in the United States, often regarded as part of a global crisis. Companies invest billions of dollars each year towards television advertising campaigns aimed at convincing audiences how their ground-breaking discovery 'battles the bulge' or somehow offers an increased health benefit. This study examined how advertisers presented health-related claims, including health and nutrient-content claims, in U.S. adult-targeted television food...
Show moreObesity is one of the major public health issues in the United States, often regarded as part of a global crisis. Companies invest billions of dollars each year towards television advertising campaigns aimed at convincing audiences how their ground-breaking discovery 'battles the bulge' or somehow offers an increased health benefit. This study examined how advertisers presented health-related claims, including health and nutrient-content claims, in U.S. adult-targeted television food commercials. The claims were compared to FTC, FDA, and USDA laws, regulations, and recommendations. A content analysis of food advertising was conducted of commercials from major and cable network programs broadcast during prime-time in the first quarter of 2009. The majority of claims match current regulations when compared to Federal references. The results show that Nutrient and Wellness claims were the most frequently cited. The type of benefit, Healthy Eating, emerged almost 3 times more than any other benefit type. This is also similar to those results which suggest advertisers' intentions were to promote overall wellness in their content delivery. As such, the Wellness Approach was identified and conceptualized, leading towards full development of a Wellness Effect theory. Implications and future research opportunities are discussed on both a theoretical and practical level.
Show less - Date Issued
- 2009
- Identifier
- CFE0002565, ucf:48260
- Format
- Document (PDF)
- PURL
- http://purl.flvc.org/ucf/fd/CFE0002565
- Title
- Inquiry as Practice for Continuous Improvement: A Framework for the Curricular Redesign of the Education Doctorate in Curriculum and Instruction Research Continuum at the University of Central Florida.
- Creator
-
Clark, Paola, Boote, David, Vitale, Thomas, Hopp, Carolyn, Swan, Bonnie, University of Central Florida
- Abstract / Description
-
This design-based research study was conducted at the University of Central Florida with the aim of informing the Education Doctorate in Curriculum and Instruction research course sequence within the College of Education and Human Performance. The main purpose of this dissertation was to enhance and enrich the Ed.D. in Curriculum and Instruction research continuum courses to ensure that they support the use of applied research and practical theory as central to the development of scholarly...
Show moreThis design-based research study was conducted at the University of Central Florida with the aim of informing the Education Doctorate in Curriculum and Instruction research course sequence within the College of Education and Human Performance. The main purpose of this dissertation was to enhance and enrich the Ed.D. in Curriculum and Instruction research continuum courses to ensure that they support the use of applied research and practical theory as central to the development of scholarly practitioners. In order to fulfill its purpose, this study addressed three main goals: clarifying the Ed.D. in Curriculum and Instruction program goals, objectives, and research continuum learning outcomes; developing research course sequence curriculum maps; and redesigning sample curriculum units for individual research courses.The curriculum mapping and redesign process was supported by research-based design choices in alignment with the practice-oriented nature of the program. These design choices included the Carnegie Project on the Education Doctorate Working Principles and Design Concepts, in particular the use of Inquiry as Practice as the main redesign framework in combination with improvement science principles. These frameworks were first used as foundations to clarify the Ed.D. in Curriculum and Instruction program goal and overall objectives. Later, user-centered design principles were applied to create faculty and student personas in order to inform the redefinition of individual research course learning outcomes. In addition, the frameworks were used to create alignment matrices and demonstrate where they supported each of the program objectives. This iterative process was carried out simultaneously with the course curriculum map redesign for each of the research continuum courses using backward design principles, the spiral curriculum model, and taking into consideration the most suitable instructional modality for learning outcomes, including the best suited education technology choices. Further, some proposed sample course units were developed in greater detail utilizing Universal Design for Learning principles and the prioritization of learning outcomes. Course contents were selected based on cognitive and reasoning learning theories pertaining to mixed method courses for professional practitioners.The developed prototypes support the continuous Ed.D. in Curriculum and Instruction curriculum redesign efforts of the program and College of Education and Human Performance at the University of Central Florida and clearly distinguish the Ed.D. in Curriculum and Instruction program from traditional, research-based doctorates. Similarly, at the national level, this study also sought to benefit other CPED-influenced professional practice programs, as they also consider the careful redesign of their research or inquiry sequences to define their programs as ones that fully address the needs of advanced professional educators. Acknowledging the limitations of this study, further studies should identifying the motivational, cognitive, and organizational causes affecting student learning outcomes. Implementing and evaluating the prototypes developed to ensure their effectiveness in preparing scholarly practitioners to act as agents of change in their professional practices.
Show less - Date Issued
- 2016
- Identifier
- CFE0006285, ucf:51585
- Format
- Document (PDF)
- PURL
- http://purl.flvc.org/ucf/fd/CFE0006285
