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- Title
- PART I : ISOLATION OF DIPENTYL 2-(4-(PENTAN-3-YL) PHENYL) MALONATE FROM SANGUISORBA OFFICINALIS LABILL PART II: SYNTHESIS OF A NOVEL FAMILY OF ETHERS OF PODOCARPIC ACID.
- Creator
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Yalavarty, Manjeera, Miles, Howard, University of Central Florida
- Abstract / Description
-
The goal of part I of this thesis was to isolate pure anti cancer compounds from the Russian plant Sanguisorba officinalis. This plant was selected for investigation because it showed 100% activity against leukemia (L1210 mouse leukemia cells) during the preliminary screening of approximately 100 Russian plant extracts. This work has resulted in the isolation of novel compound 11 using traditional chromatography techniques. Compound 11 was characterized with spectroscopic techniques such as...
Show moreThe goal of part I of this thesis was to isolate pure anti cancer compounds from the Russian plant Sanguisorba officinalis. This plant was selected for investigation because it showed 100% activity against leukemia (L1210 mouse leukemia cells) during the preliminary screening of approximately 100 Russian plant extracts. This work has resulted in the isolation of novel compound 11 using traditional chromatography techniques. Compound 11 was characterized with spectroscopic techniques such as IR, 1H NMR, 13C NMR, DEPT, DQCOSY and MS. Compound 11 was assigned the structure dipentyl 2-(4-(pentan-3-yl) phenyl) malonate. The goal of part II of this thesis was to synthesize novel ethers of podocarpic acid. Ethers are of great interest in biological studies and pharmaceutical applications because of their wide variety of uses in the treatment of various diseases. A novel family of ether derivatives was synthesized using podocarpic acid (a natural tricyclic diterpene derived from podocarpus species) as a template. Novel ether derivatives of podocarpic acid were synthesized from podocarpic acid in three steps. The first step involved methylation of podocarpic acid with dimethyl sulfate to form methyl-o-methyl podocarpate. The second step was iodination of methyl-o-methyl podocarpate with iodine in presence of a mercury catalyst to form 13-iodo methyl-o-methyl podocarpate. This was followed by formation of novel aliphatic ethers using a copper catalyst. Thus this research had led to the discovery of new methodology for synthesis of three novel aliphatic ether derivatives of podocarpic acid. These ethers will be tested for their biological activity against various types of cancer, tuberculosis by National Institutes of Health.
Show less - Date Issued
- 2005
- Identifier
- CFE0000625, ucf:46551
- Format
- Document (PDF)
- PURL
- http://purl.flvc.org/ucf/fd/CFE0000625
- Title
- MULTIPLE ASPECTS OF NATURAL KILLER CELL EXPANSION IN RELEVANCE TO IMMUNOTHERAPY FOR HEMATOLOGIC MALIGNANCIES.
- Creator
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Colosimo, Dominic, Borgon, Robert, University of Central Florida
- Abstract / Description
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Natural Killer (NK) cells are a subset of lymphocytes that regulate adaptive immune responses and utilize "missing self" recognition to activate anti-tumor and anti-viral cytotoxicity. Clinical research, as well as murine and ex vivo models, have shown that a variety of NK cell applications have proven useful as immunotherapeutic treatments for patients with hematologic malignancies. However, the selective expansion of NK cells to yield relevant amounts of these lymphocytes has been a major...
Show moreNatural Killer (NK) cells are a subset of lymphocytes that regulate adaptive immune responses and utilize "missing self" recognition to activate anti-tumor and anti-viral cytotoxicity. Clinical research, as well as murine and ex vivo models, have shown that a variety of NK cell applications have proven useful as immunotherapeutic treatments for patients with hematologic malignancies. However, the selective expansion of NK cells to yield relevant amounts of these lymphocytes has been a major hurdle in the development of methods for clinical therapeutic use. Here, we demonstrate a novel ex vivo expansion method utilizing k562 leukemic cell lines and soluble cytokines as well as a novel method utilizing isolated plasma membranes of genetically engineered tumor cell lines that could be of relevance to in vivo NK cell expansion. Also, the ligand expression by canonical feeder cell lines used for NK cell expansion and our isolated plasma membranes were compared via ligand quantification by western blot quantification of 4-1BB ligand. In an adjunct study, we sought to better characterize these expansion environments by investigating the glucose metabolism of NK cells using fluorescent glucose analog 2-(N-(7-Nitrobenz-2-oxa-1, 3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG) and the glycolysis inhibitor 2-Deoxy-D-Glucose (2-DG).
Show less - Date Issued
- 2012
- Identifier
- CFH0004252, ucf:44917
- Format
- Document (PDF)
- PURL
- http://purl.flvc.org/ucf/fd/CFH0004252
- Title
- A mathematical model for feral cat ecology with application to disease.
- Creator
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Sharpe, Jeff, Nevai, A, Shuai, Zhisheng, Qi, Yuanwei, Quintana-Ascencio, Pedro, University of Central Florida
- Abstract / Description
-
We formulate and analyze a mathematical model for feral cats living in an isolated colony. The model contains compartments for kittens, adult females and adult males. Kittens are born at a rate proportional to the population of adult females and mature at equal rates into adult females and adult males. Adults compete with each other in a manner analogous to Lotka-Volterra competition. This competition comes in four forms, classified by gender. Native house cats, and their effects are also...
Show moreWe formulate and analyze a mathematical model for feral cats living in an isolated colony. The model contains compartments for kittens, adult females and adult males. Kittens are born at a rate proportional to the population of adult females and mature at equal rates into adult females and adult males. Adults compete with each other in a manner analogous to Lotka-Volterra competition. This competition comes in four forms, classified by gender. Native house cats, and their effects are also considered, including additional competition and abandonment into the feral population. Control measures are also modeled in the form of per-capita removal rates. We compute the net reproduction number (R_0) for the colony and consider its influence. In the absence of abandonment, if R_0(>)1, the population always persists at a positive equilibrium and if R_0 (<)= 1, the population always tends toward local extinction. This work will be referred to as the core model.The model is then expanded to include a set of colonies (patches) such as those in the core model (this time neglecting the effect of abandonment). Adult females and kittens remain in their native patch while adult males spend a fixed proportion of their time in each patch. Adult females experience competition from both the adult females living in the same patch as well as the visiting adult males. The proportion of adult males in patch j suffer competition from both adult females resident to that patch as well the proportion of adult males also in the patch. We formulate a net reproduction number for each patch (a patch reproduction number) R_j. If R_j(>)1 for at least one patch, then the collective population always persists at some nontrivial (but possibly semitrivial) steady state. We consider the number of possible steady states and their properties. This work will be referred to as the patch model.Finally, the core model is expanded to include the introduction of the feline leukemia virus. Since this disease has many modes of transmission, each of which depends on the host's gender and life-stage, we regard this as a model disease. A basic reproduction number R_0 for the disease is defined and analyzed. Vaccination terms are included and their role in disease propagation is analyzed. Necessary and sufficient conditions are given under which the disease-free equilibrium is stable.
Show less - Date Issued
- 2016
- Identifier
- CFE0006502, ucf:51389
- Format
- Document (PDF)
- PURL
- http://purl.flvc.org/ucf/fd/CFE0006502
