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(1 - 5 of 5)
- Title
- THE ROLE OF THE INTERMEMBRANE DOMAIN OF MULAN IN MITOPHAGY AND CELL DEATH.
- Creator
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Herbert, Jared M, Zervos, Antonis S., University of Central Florida
- Abstract / Description
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Mulan is an E3 ubiquitin ligase and an E3 SUMO ligase embedded in the outer mitochondrial membrane. Mulan plays a major role in various cell processes including cell growth, mitophagy, apoptosis, and mitochondrial dynamics. In addition, its deregulation isinvolved in the development and progression of several human disorders such as neurodegeneration and heart disease. There are two main discernible domains in Mulan: a large cytoplasmic domain that encodes the RING-finger motif and carries...
Show moreMulan is an E3 ubiquitin ligase and an E3 SUMO ligase embedded in the outer mitochondrial membrane. Mulan plays a major role in various cell processes including cell growth, mitophagy, apoptosis, and mitochondrial dynamics. In addition, its deregulation isinvolved in the development and progression of several human disorders such as neurodegeneration and heart disease. There are two main discernible domains in Mulan: a large cytoplasmic domain that encodes the RING-finger motif and carries out the catalytic activity of the protein; the second domain of Mulan is exposed to the intermembrane space of mitochondria, and its function remains unknown. This part of Mulan is also referred to as the BAM domain and is expected to have a significant function since its amino acid sequence has been conserved through evolution and is found in bacteria, animals, and plants. The purpose of this study is to isolate and characterize potential binding partner proteins of the BAM domain using the yeast two-hybrid system. These studies are expected to provide new information on the physiological function of this domain and how it is potentially used to modulate the ligase activity of Mulan.
Show less - Date Issued
- 2016
- Identifier
- CFH0000235, ucf:44682
- Format
- Document (PDF)
- PURL
- http://purl.flvc.org/ucf/fd/CFH0000235
- Title
- THE EFFECTS OF BAM AS AN ADSORPTIVE MEDIA ON PHOSPHORUS REMOVAL IN STORMWATER.
- Creator
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Salamah, Sultan, Randall, Andrew, Duranceau, Steven, Chopra, Manoj, University of Central Florida
- Abstract / Description
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To maintain the quality of receiving water bodies, it is desirable to remove total phosphorus (TP) in stormwater runoff. Many media filtration technologies have been developed to achieve TP and soluble reactive phosphorus (SRP) removal. Efficient media adsorption is essential to insure control of stormwater phosphorus inputs to the receiving water body. This project develops and analyzes a functionalized Biosorption Media (BAM) to remove phosphorus species from stormwater runoff. One goal of...
Show moreTo maintain the quality of receiving water bodies, it is desirable to remove total phosphorus (TP) in stormwater runoff. Many media filtration technologies have been developed to achieve TP and soluble reactive phosphorus (SRP) removal. Efficient media adsorption is essential to insure control of stormwater phosphorus inputs to the receiving water body. This project develops and analyzes a functionalized Biosorption Media (BAM) to remove phosphorus species from stormwater runoff. One goal of this project is to find the BAM values for coefficients such as maximum adsorption capacity (QM: 4.35E-05) for the media through SRP isotherm equilibrium experiments using the Langmuir and Freundlich models. In addition, an upflow column experiment was also performed to study BAM nutrient removal from stormwater runoff. Finally, the information from the isotherm and the column experiments are used to estimate the life expectancy or quantity required of the media, and to define the effectiveness of BAM in phosphorus removal. The result of this study shows that BAM is a feasible stormwater treatment that can remove 60% SRP and (>)40% TP at temperature between 21-23 (&)deg;C. The media is adequately modeled by both the Langmuir and the Freundlich models over the concentration range of interest in stormwater.
Show less - Date Issued
- 2014
- Identifier
- CFE0005244, ucf:50589
- Format
- Document (PDF)
- PURL
- http://purl.flvc.org/ucf/fd/CFE0005244
- Title
- Comparison of a modified and traditional rapid infiltration basin for treatment and control of nutrients in wastewater effluent.
- Creator
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Cormier, Jessica, Duranceau, Steven, Wang, Dingbao, Sadmani, A H M Anwar, University of Central Florida
- Abstract / Description
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Rapid infiltration basins (RIB) have been historically used in Florida for groundwater recharge, effluent disposal, or a combination of both. However, this technique has proven ineffective in providing nitrogen control unless the RIB is modified in some manner. In this study, a traditional RIB was compared to a modified RIB constructed with manufactured biosorption activated media (BAM) to evaluate nitrate removal from reclaimed water. The RIBs are used for reclaimed and excess storm water...
Show moreRapid infiltration basins (RIB) have been historically used in Florida for groundwater recharge, effluent disposal, or a combination of both. However, this technique has proven ineffective in providing nitrogen control unless the RIB is modified in some manner. In this study, a traditional RIB was compared to a modified RIB constructed with manufactured biosorption activated media (BAM) to evaluate nitrate removal from reclaimed water. The RIBs are used for reclaimed and excess storm water disposal. Few, if any, studies have been published where BAM-modified RIBs have been used for this purpose. In this work, a mixture of clay, tire crumb, and sand (CTS) was selected to serve as the BAM material (Bold and Gold(TM) CTS media). Each RIB was constructed with two feet of either sand or BAM, covering more than 43,600 square feet of surface area. The BAM-modified RIB had an initial 90 pounds per cubic-foot in-place density, and the density of the control RIB approximated about 94 pounds per cubic-foot. Over an eight-month period, loadings to the BAM RIB and control RIB approximated 5.4 million gallons (MG) per acre each. Water samples, collected from lysimeters installed below the 2-foot of sand or BAM materials, were gathered monthly during 2017 (except for September and October due to the impacts of hurricane Irma); these samples were analyzed for water quality to determine nitrate removal. Soil moisture and weather data were also collected over the study period. This study demonstrated the nitrate removal effectiveness of a field-scale BAM-modified RIB as compared to a traditional field-scale sand-based RIB. Results suggest that BAM removed 30 percent more nitrates than the Control (78% and 47%, respectively) under the conditions of the study. Furthermore, BAM removed higher percentages of TN (31%) and TP (62%) than the Control (12% and 28%, respectively).
Show less - Date Issued
- 2018
- Identifier
- CFE0007566, ucf:52583
- Format
- Document (PDF)
- PURL
- http://purl.flvc.org/ucf/fd/CFE0007566
- Title
- Evaluation of Biosorption Activated Media Under Roadside Swales for Stormwater Quality Improvement & Harvesting.
- Creator
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Hood, Andrew, Chopra, Manoj, Wanielista, Martin, Randall, Andrew, University of Central Florida
- Abstract / Description
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Stormwater runoff from highways is a source of pollution to surface water bodies and groundwater. This project develops a bio-detention treatment and harvesting system that is incorporated into roadside swales. The bio-detention system uses Bold & Gold(TM), a type of biosorption activated media (BAM), to remove nutrients from simulated highway runoff and then store the water in underground vaults for infiltration, controlled discharge, and/or irrigation and other non-potable applications. In...
Show moreStormwater runoff from highways is a source of pollution to surface water bodies and groundwater. This project develops a bio-detention treatment and harvesting system that is incorporated into roadside swales. The bio-detention system uses Bold & Gold(TM), a type of biosorption activated media (BAM), to remove nutrients from simulated highway runoff and then store the water in underground vaults for infiltration, controlled discharge, and/or irrigation and other non-potable applications. In order to design a bio-detention system, media characteristics and media/water quality relationships are required. Media characteristics determined through testing include: specific gravity, permeability, infiltration, maximum dry density, moisture content of maximum dry density, and particle-size distribution. One of the goals of this experiment is to compare the nitrogen and phosphorous species concentrations in the effluent of BAM to sandy soil for simulated highway runoff. Field scale experiments are done on an elevated test bed that simulates a typical roadway with a swale. The swale portion of the test bed is split into halves using BAM and sandy soil. The simulated stormwater flows over a concrete section, which simulates a roadway, and then over either sod covered sandy soil or BAM. One, one and a half, and three inch storms are each simulated three times with a duration of 30 minutes each. During the simulated storm event, initial samples of the runoff (influent) are taken. The test bed is allowed to drain for two hours after the rainfall event and then samples of each of the net effluents are taken. In addition to the field scale water quality testing, column tests are also preformed on the sandy soil and Bold & Gold(TM) without sod present. Sod farms typically use fertilizer to increase production, thus it is reasonable to assume that the sod will leach nutrients into the soils on the test bed, especially during the initial test runs. The purpose of the column tests is to obtain a general idea of what percentage removals of total phosphorus and total nitrogen are obtained by the sandy soil and Bold & Gold(TM). It is shown that the Bold & Gold(TM) media effluent has significantly lower concentrations of total nitrogen and total phosphorus compared to the effluent of the sandy soil based on an 80% confidence level. The Bold & Gold(TM) has a 41% lower average effluent concentration of total nitrogen than the sandy soil. The Bold & Gold(TM) media has a 78% lower average effluent concentration of total phosphorus than the sandy soil. Using both the column test data in combination with the field scale data, it is determined that the Bold & Gold(TM) BAM system has a total phosphorus removal efficiency of 71%. The removal efficiency is increased when stormwater harvesting is considered. A total phosphorus reduction of 94% is achieved in the bio-detention & harvesting swale system sample design problem.
Show less - Date Issued
- 2012
- Identifier
- CFE0004312, ucf:52869
- Format
- Document (PDF)
- PURL
- http://purl.flvc.org/ucf/fd/CFE0004312
- Title
- An Assessment of Biosorption Activated Media for the Removal of Pollutants in Up-Flow Stormwater Treatment Systems.
- Creator
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Hood, Andrew, Randall, Andrew, Wanielista, Martin, Chopra, Manoj, O'Reilly, Andrew, Moore, Sean, University of Central Florida
- Abstract / Description
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Nitrogen and phosphorus are often the limiting nutrients for marine and freshwater systems respectively. Additionally, stormwater often contains elevated levels of pathogens which can pollute the receiving water body and impact reuse applications [1-4]. The reduction of limiting nutrients and pathogens is a common primary target for stormwater best management practices (BMPs) [5]. Traditional BMPs, such as retention/detention treatment ponds require large footprints and may not be practical...
Show moreNitrogen and phosphorus are often the limiting nutrients for marine and freshwater systems respectively. Additionally, stormwater often contains elevated levels of pathogens which can pollute the receiving water body and impact reuse applications [1-4]. The reduction of limiting nutrients and pathogens is a common primary target for stormwater best management practices (BMPs) [5]. Traditional BMPs, such as retention/detention treatment ponds require large footprints and may not be practical in ultra-urban environments where above ground space is limited. Upflow filters utilizing biosorption activated media (BAM) that can be placed underground offer a small footprint alternative. Additionally, BAM upflow filters can be installed at the discharge point of traditional stormwater ponds to provide further treatment. This research simulated stormwater that had already been treated for solids removal; thus, most of the nutrients and solids in the influent were assumed to be as non-settable suspended solids or dissolved solids. Three different BAM mixtures in an upflow filter configuration were compared for the parameters of nitrogen, phosphorus, total coliform, E. coli, and heterotrophic plate count (HPC). Additionally, genetic testing was conducted using Polymerase Chain Reaction (PCR), in conjunction with a nitrogen mass balance, to determine if Anammox was a significant player in the nitrogen removal. The columns were run at both 22-minute and 220-minute Empty Bed Contact Times (EBCTs). All the BAM mixtures analyzed were shown to be capable at the removal of nitrogen, phosphorus, and total coliform during both the 22-minute and 220-minute EBCTs, with BAM #1 having the highest removal performance for all three parameters during both EBCTs. All BAM mixtures experienced an increase in HPC. Additionally, PCR analysis confirmed the presence of Anammox in the biofilm and via mass balance it was determined that the biological nitrogen removal was due to Anammox and endogenous denitrification with Anammox being a significant mechanism.
Show less - Date Issued
- 2019
- Identifier
- CFE0007817, ucf:52875
- Format
- Document (PDF)
- PURL
- http://purl.flvc.org/ucf/fd/CFE0007817
