Current Search: Cancer (x)
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Title
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THE INFLUENCE OF 3D POROUS CHITOSAN-ALGINATE BIOMATERIAL SCAFFOLD PROPERTIES ON THE BEHAVIOR OF BREAST CANCER CELLS.
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Creator
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Le, Minh-Chau N., Steward, Robert L., Florczyk, Stephen J., University of Central Florida
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Abstract / Description
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The tumor microenvironment plays an important role in regulating cancer cell behavior. The tumor microenvironment describes the cancer cells, and the surrounding endothelial cells, fibroblasts, and mesenchymal stem cells, along with the extracellular matrix (ECM). The tumor microenvironment stiffens as cancer undergoes malignant progression, providing biophysical cues that promote invasive, metastatic cellular behaviors. This project investigated the influence of three dimensional (3D)...
Show moreThe tumor microenvironment plays an important role in regulating cancer cell behavior. The tumor microenvironment describes the cancer cells, and the surrounding endothelial cells, fibroblasts, and mesenchymal stem cells, along with the extracellular matrix (ECM). The tumor microenvironment stiffens as cancer undergoes malignant progression, providing biophysical cues that promote invasive, metastatic cellular behaviors. This project investigated the influence of three dimensional (3D) chitosan-alginate (CA) scaffold stiffness on the morphology, growth, and migration of green fluorescent protein (GFP) � transfected MDA-MB-231 (231-GFP) breast cancer (BCa) cells. The CA scaffolds were produced by the freeze casting method at three concentrations, 2 wt%, 4 wt%, and 6 wt% to provide different stiffness culture substrates. The CA scaffold material properties were characterized using scanning electron microscopy imaging for pore structure and compression testing for Young's Modulus. The BCa cell cultures were characterized at day 1, 3, and 7 timepoints using Alamar Blue assay for cell number, fluorescence imaging for cell morphology, and single-cell tracking for cell migration. Pore size calculations using SEM imaging yielded pore sizes of 253.29 +/- 52.45 [micro]m, 209.55 +/- 21.46 [micro]m, and 216.83 +/- 32.63 [micro]m for 2 wt%, 4 wt%, and 6 wt%, respectively. Compression testing of the CA scaffolds yielded Young's Modulus values of 0.064 +/- 0.008 kPa, 2.365 +/- 0.32 kPa and 3.30 +/- 0.415 kPa for 2 wt%, 4 wt%, and 6 wt% CA scaffolds, respectively. The results showed no significant difference in cell number among the 3D CA scaffold groups. However, the 231-GFP cells cultured in 2 wt% CA scaffolds possessed greater cellular size, area, perimeter, and lower cellular circularity compared to those in 4 wt% and 6 wt% CA scaffolds, suggesting a more prominent presence of cell clusters in softer substrates compared to stiffer substrates. The results also showed cells in 6 wt% CA having a higher average cell migration speed compared to those in 2 wt% and 4 wt% CA scaffolds, indicating a positive relationship between substrate stiffness and cell migration velocity. Findings from this experiment may contribute to the development of enhanced in vitro 3D breast tumor models for basic cancer research using 3D porous biomaterial scaffolds.
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Date Issued
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2019
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Identifier
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CFH2000492, ucf:45626
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Format
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Document (PDF)
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PURL
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http://purl.flvc.org/ucf/fd/CFH2000492
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Title
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TO EVALUATE THE FUNCTION OF THE OXYTOCIN RECEPTOR IN THE CONTEXT OF OVARIAN CANCER CELL MICROENVIRONMENT TO DETERMINE IF OXYTOCIN CAN INDUCE AN ANTI-INFLAMMATORY RESPONSE.
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Creator
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Schachner, Benjamin I, Samsam, Mohtashem, University of Central Florida
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Abstract / Description
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The treatment of most cancers can still be considered inadequate despite the steady progress being made. A prime example of this issue is with epithelial ovarian cancers; this disease presents a significant issue, with a 5-year survival rate of 46% and a survival rate of 28% in patients that develop metastatic disease. Since ovarian cancer has such a high mortality rate, effective treatment modalities are necessary to prolong the quality of life after diagnosis. Psychosocial stress is related...
Show moreThe treatment of most cancers can still be considered inadequate despite the steady progress being made. A prime example of this issue is with epithelial ovarian cancers; this disease presents a significant issue, with a 5-year survival rate of 46% and a survival rate of 28% in patients that develop metastatic disease. Since ovarian cancer has such a high mortality rate, effective treatment modalities are necessary to prolong the quality of life after diagnosis. Psychosocial stress is related to the progression, proliferation, and migration in cancer patients, but the mechanisms of this relationship are not fully understood. The present in vitro study investigated the ability of oxytocin, a neuropeptide associated with social support, to attenuate the stress response. Catecholamines, a subclass of stress hormones, were used to simulate the stress induced inflammation process in ovarian cancer cells. To evaluate oxytocin's capacity to attenuate the stress response, the ovarian cancer cell lines SKOV3, HEYA8, OVCAR8, and OV432 were separately treated with the presence or absence of catecholamines with the addition of oxytocin. Protein expression of the oxytocin receptor was investigated using a western blot protocol. Oxytocin receptor, oxytocin, and IL-6 mRNA expression was evaluated by quantitative PCR. Treatment with Oxytocin attenuated the inflammatory response resulting from catecholamine treatment. The oxytocin receptor gene and protein were present in each cell line, suggesting that oxytocin has an anti-inflammatory role in the tumor microenvironment in ovarian cancer patients. These results provide a mechanism by which social support, working through the release of oxytocin, promotes an anti-inflammatory process in ovarian cancer patients. This study may shed light into new pharmacological approaches for the treatment of ovarian cancer.
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Date Issued
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2017
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Identifier
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CFH2000344, ucf:45809
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Format
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Document (PDF)
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PURL
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http://purl.flvc.org/ucf/fd/CFH2000344
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Title
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STUDY OF THE EGFR, SRC AND STAT3 PATHWAY IN PANCREATIC CANCER.
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Creator
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Jaganathan, Soumya, Turkson, James, University of Central Florida
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Abstract / Description
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Cancer is associated with many molecular aberrations that support the malignant phenotype. In that regard, aberrant activation of epidermal growth factor receptor (EGFR), Src, and signal transducer and activator of transcription 3 (Stat3) occur concurrently in pancreatic cancer and are implicated in the disease phenotype. Notwithstanding, increasing evidence indicates that therapies that target only EGFR or Src are rather ineffective in modulating the cancer phenotype. The poor therapeutic...
Show moreCancer is associated with many molecular aberrations that support the malignant phenotype. In that regard, aberrant activation of epidermal growth factor receptor (EGFR), Src, and signal transducer and activator of transcription 3 (Stat3) occur concurrently in pancreatic cancer and are implicated in the disease phenotype. Notwithstanding, increasing evidence indicates that therapies that target only EGFR or Src are rather ineffective in modulating the cancer phenotype. The poor therapeutic outcome of the monotherapies targeting EGFR or Src may in part be due to the increased incidence of signaling cross-talks among aberrant signaling pathways in cancer. Molecular details of the signaling integration between EGFR, Src and Stat3, however, are lacking. Understanding how the aberrant EGFR, Src and Stat3 pathways are integrated in pancreatic cancer would facilitate the design of effective multiple-targeted, clinically feasible therapeutic modalities. Our study shows that in pancreatic cancer cell lines, aberrant Src activity promotes abnormal EGFR activation through the phosphorylation of the EGFR motifs, Tyr845, Tyr1068 and Tyr1086. Furthermore, aberrantly-active EGFR and Src together induce constitutive activation of Stat3 in pancreatic cancer cells. Evidence further shows that EGFR, Src and Stat3 physically associated into a heteromeric complex. Significantly, the EGFR, Src and Stat3 heteromeric complex is detectable in the nucleus and functions as a transcriptionally-active complex to induce the c-Myc gene. Of therapeutic significance, the concurrent inhibition of Stat3 and EGFR or Src promoted greater viability loss and apoptosis of pancreatic cancer cells in vitro, and induced stronger tumor growth inhibition in xenografts of human pancreatic cancer. Altogether, our studies suggest that the heteromeric EGFR, Src, and Stat3 complex may serve as an additional novel mechanism of support of the pancreatic cancer phenotype. Furthermore, our studies provide evidence that the concurrent targeting of Stat3 and EGFR or Stat3 and Src could be a more effective therapeutic approach for human pancreatic cancer.
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Date Issued
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2010
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Identifier
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CFE0003417, ucf:48383
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Format
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Document (PDF)
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PURL
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http://purl.flvc.org/ucf/fd/CFE0003417
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Title
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EFFECTS OF A PLANT-BASED VEGAN DIET ON THE RISK OF CANCER: AN INTEGRATIVE REVIEW OF THE LITERATURE.
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Creator
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Silavent, Chelsie M, Bushy, Angeline, University of Central Florida
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Abstract / Description
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Cancer, an aggressive chronic disease, impacts the lives of millions of people every day. There are numerous proposed triggers related to the diagnosis and much remains to be learned about the causes. Associated with this disease's variability is the challenge to identify a single causative agent that lead to its prevention. Specific topics that need additional evidence relate to environmental factors and lifestyle behaviors in the development, treatment and, in some instances, even...
Show moreCancer, an aggressive chronic disease, impacts the lives of millions of people every day. There are numerous proposed triggers related to the diagnosis and much remains to be learned about the causes. Associated with this disease's variability is the challenge to identify a single causative agent that lead to its prevention. Specific topics that need additional evidence relate to environmental factors and lifestyle behaviors in the development, treatment and, in some instances, even suppression of disease progression. Specifically, a diet that excludes animal-based products but consists of a variety of fruits and vegetables (i.e., plant-based vegan diet) is reported to retard disease progression among some individuals diagnosed with cancer. The purpose of this thesis was to examine the effects of a plant-based vegan diet as a treatment approach with individuals who have been diagnosed with cancer. The methodology included a systematic review of literature focusing on use of a plant-based vegan diet on cancer risks in research articles published in peer reviewed journals from 2006 to 2018. Consistent findings include evidence that a diet high in fruits, vegetables, whole grains, and minimal amounts of animal products showed a decrease risk of the development of various types of cancer. Recommendations for nursing education, practice, policy and research are discussed.
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Date Issued
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2019
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Identifier
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CFH2000558, ucf:45619
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Format
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Document (PDF)
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PURL
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http://purl.flvc.org/ucf/fd/CFH2000558
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Title
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STUDIES OF NORSPERMIDINE UPTAKE IN DROSOPHILA SUGGEST THE EXISTENCE OF MULTIPLE POLYAMINE TRANSPORT PATHWAYS.
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Creator
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Dieffenbach, Michael, Von Kalm, Laurence, Teter, Kenneth, University of Central Florida
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Abstract / Description
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Polyamines are a class of essential nutrients involved in many basic cellular processes such as gene expression, cell proliferation, and apoptosis. Without polyamines, cell growth is delayed or halted. Cancerous cells require an abundance of polyamines through a combination of synthesis and transport from the extracellular environment. An FDA-approved drug, D,L-?-difluoromethylornithine (DFMO), blocks polyamine synthesis but is ineffective at inhibiting cell growth due to polyamine transport....
Show morePolyamines are a class of essential nutrients involved in many basic cellular processes such as gene expression, cell proliferation, and apoptosis. Without polyamines, cell growth is delayed or halted. Cancerous cells require an abundance of polyamines through a combination of synthesis and transport from the extracellular environment. An FDA-approved drug, D,L-?-difluoromethylornithine (DFMO), blocks polyamine synthesis but is ineffective at inhibiting cell growth due to polyamine transport. Thus, there is a need to develop drugs that inhibit polyamine transport to use in combination with DFMO. Surprisingly, little is known about the polyamine transport system in humans and other eukaryotes. Understanding the transport system would allow us to identify compounds that inhibit polyamine transport, which could then be used in tandem with DFMO to treat cancer. Our laboratory has identified one gene in Drosophila, called CG32000, as a component of this transport system, and numerous other candidate genes remain to be tested. To better characterize this system, this project investigated the ability of the Drosophila transport system to take up a toxic polyamine analogue called norspermidine, with the initial goal of developing a new screening method to find polyamine transport genes. My experiments have demonstrated significant differences in norspermidine uptake and toxicity between C. elegans and Drosophila which may imply a secondary polyamine transport system in higher eukaryotes. In the long term, it is hoped that this thesis will facilitate the development of more effective cancer medications by providing new information about the polyamine transport system.
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Date Issued
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2018
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Identifier
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CFH2000294, ucf:45869
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Format
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Document (PDF)
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PURL
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http://purl.flvc.org/ucf/fd/CFH2000294
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Title
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SELF-EFFICACY AND COPING IN TRANSITION OF CARE AFTER REMISSION OF CANCER IN ADOLESCENTS.
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Creator
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McDonnell, Leah M, D' Amato-Kubiet, Leslee, University of Central Florida
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Abstract / Description
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The improvement in cancer remission rates in children and adolescents due to advances in cancer treatment and therapy has led to the development of guidelines that address long-term follow up for survivors of childhood cancers. Adolescents often experience negative emotions related to the fear of uncertainty about long-term survival after cancer remission, yet often report feelings of hope and optimism for the future more than adult cancer survivors. The purpose of this study was to...
Show moreThe improvement in cancer remission rates in children and adolescents due to advances in cancer treatment and therapy has led to the development of guidelines that address long-term follow up for survivors of childhood cancers. Adolescents often experience negative emotions related to the fear of uncertainty about long-term survival after cancer remission, yet often report feelings of hope and optimism for the future more than adult cancer survivors. The purpose of this study was to understand the role of self-efficacy and coping in adolescents after remission of cancer. A secondary purpose was to analyze which coping strategies supported long-term survival goals after cancer remission in adolescent populations. A systematic literature review was conducted from the following online databases: Cumulative Index to Nursing and Allied Health Literature (CINAHL), Medical Literature On-line (MEDLINE), Education Resources Information Center (ERIC), and PsycInfo. Selected articles included those published between 2000-2016 that were written in English and were peer-reviewed. The results of the study revealed that most adolescents with cancer remission do not experience long term psychosocial issues related to their cancer diagnosis and treatment. However, a large percentage of adolescent cancer survivors report intermittent depression, suicidal ideation and a lower quality of life due to survival after remission. The literature indicates that multiple, integrative forms of behavioral therapy: cognitive, psychosocial, and family based treatment models, help to enhance long term quality of life in adolescent cancer survivors. Strategies that use positive coping methods and improve self-efficacy related to long term survival after remission have demonstrated improvement in psychosocial behaviors in adolescents and promote a better outlook on planning for the future. Future research that analyzes the most effective coping skills to practice after cancer remission and that optimize self-efficacy related to long term survival can positively influence quality of life for adolescent cancer survivors.
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Date Issued
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2016
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Identifier
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CFH2000049, ucf:45552
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Format
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Document (PDF)
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PURL
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http://purl.flvc.org/ucf/fd/CFH2000049
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Title
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THE SYNCHRONICITY OF HOPE AND ENHANCED QUALITY OF LIFE IN TERMINAL CANCER.
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Creator
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Terry, Brianna M, Chase, Susan, University of Central Florida
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Abstract / Description
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Cancer is the second leading cause of death in the United States and a leading cause of death worldwide. The rate of mortality is currently approximately 171.2 out of every 100,000 individuals with a terminal cancer diagnosis annually. Individuals with terminal cancer diagnoses facing probable mortality utilize various coping mechanisms or internal resources in an attempt to maintain an internal sense of well-being, commonly referred to as quality of life (QOL). The purpose of this literature...
Show moreCancer is the second leading cause of death in the United States and a leading cause of death worldwide. The rate of mortality is currently approximately 171.2 out of every 100,000 individuals with a terminal cancer diagnosis annually. Individuals with terminal cancer diagnoses facing probable mortality utilize various coping mechanisms or internal resources in an attempt to maintain an internal sense of well-being, commonly referred to as quality of life (QOL). The purpose of this literature review was to investigate themes prevalent in the literature pertaining to internal coping mechanisms and analyze any correlation or causation linking these resources to a change in QOL in individuals with a terminal cancer diagnosis. The secondary purpose of this review was to interpret and define the healthcare provider's role in supporting this relationship. A systematic review of the literature was conducted from multiple online databases. Multiple studies related to the overarching themes of internal resources and QOL for individuals with a terminal cancer diagnosis were selected for the review. Results revealed major themes pertaining to correlation between hope and QOL. Studies which analyzed the relationship between hope and QOL found a positive correlation. The literature suggests that healthcare providers are capable of facilitating this relationship between hope and QOL. Healthcare provider facilitation of the relationship between hope and QOL is valuable in the clinical setting, and can aid an individual in achieving a desirable QOL.
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Date Issued
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2016
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Identifier
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CFH2000075, ucf:45520
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Format
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Document (PDF)
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PURL
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http://purl.flvc.org/ucf/fd/CFH2000075
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Title
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Learning Algorithms for Fat Quantification and Tumor Characterization.
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Creator
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Hussein, Sarfaraz, Bagci, Ulas, Shah, Mubarak, Heinrich, Mark, Pensky, Marianna, University of Central Florida
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Abstract / Description
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Obesity is one of the most prevalent health conditions. About 30% of the world's and over 70% of the United States' adult populations are either overweight or obese, causing an increased risk for cardiovascular diseases, diabetes, and certain types of cancer. Among all cancers, lung cancer is the leading cause of death, whereas pancreatic cancer has the poorest prognosis among all major cancers. Early diagnosis of these cancers can save lives. This dissertation contributes towards the...
Show moreObesity is one of the most prevalent health conditions. About 30% of the world's and over 70% of the United States' adult populations are either overweight or obese, causing an increased risk for cardiovascular diseases, diabetes, and certain types of cancer. Among all cancers, lung cancer is the leading cause of death, whereas pancreatic cancer has the poorest prognosis among all major cancers. Early diagnosis of these cancers can save lives. This dissertation contributes towards the development of computer-aided diagnosis tools in order to aid clinicians in establishing the quantitative relationship between obesity and cancers. With respect to obesity and metabolism, in the first part of the dissertation, we specifically focus on the segmentation and quantification of white and brown adipose tissue. For cancer diagnosis, we perform analysis on two important cases: lung cancer and Intraductal Papillary Mucinous Neoplasm (IPMN), a precursor to pancreatic cancer. This dissertation proposes an automatic body region detection method trained with only a single example. Then a new fat quantification approach is proposed which is based on geometric and appearance characteristics. For the segmentation of brown fat, a PET-guided CT co-segmentation method is presented. With different variants of Convolutional Neural Networks (CNN), supervised learning strategies are proposed for the automatic diagnosis of lung nodules and IPMN. In order to address the unavailability of a large number of labeled examples required for training, unsupervised learning approaches for cancer diagnosis without explicit labeling are proposed. We evaluate our proposed approaches (both supervised and unsupervised) on two different tumor diagnosis challenges: lung and pancreas with 1018 CT and 171 MRI scans respectively. The proposed segmentation, quantification and diagnosis approaches explore the important adiposity-cancer association and help pave the way towards improved diagnostic decision making in routine clinical practice.
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Date Issued
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2018
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Identifier
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CFE0007196, ucf:52288
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Format
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Document (PDF)
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PURL
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http://purl.flvc.org/ucf/fd/CFE0007196
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Title
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The Role of Type-I Interferon in Limiting Spread and Killing of an Oncolytic RNA Virus in Prostate Cells.
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Creator
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Kedarinath, Kritika, Parks, Griffith, Chakrabarti, Ratna, Altomare, Deborah, University of Central Florida
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Abstract / Description
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Prostate cancer is the second most prevalent cancer amongst men and there is an urgent need to address viable therapeutic options for its treatment. Development of viruses which target and kill cancer cells has gained momentum due to the first FDA approved oncolytic virus for treating human cancer patients. Our previous work with the RNA virus, Parainfluenza Virus 5 (PIV5), has led to the generation of mutants that are potential candidates for oncolytic viruses: 1) the hyperfusogenic (P/V/F)...
Show moreProstate cancer is the second most prevalent cancer amongst men and there is an urgent need to address viable therapeutic options for its treatment. Development of viruses which target and kill cancer cells has gained momentum due to the first FDA approved oncolytic virus for treating human cancer patients. Our previous work with the RNA virus, Parainfluenza Virus 5 (PIV5), has led to the generation of mutants that are potential candidates for oncolytic viruses: 1) the hyperfusogenic (P/V/F) mutant has a mutated P/V and fusion gene which activates anti-viral responses and causes massive cell-cell fusion respectively, and 2) the Leader mutant has a mutated viral genomic promoter which kills cells due to overactive viral gene expression. The P/V/F mutant has shown effectiveness in reducing prostate tumor burden in a mouse model system, however, the specificity of these viruses is unclear, i.e. targeting cancerous prostate cells while leaving uninvolved cells unaffected. In this study, we addressed how these PIV5 mutants replicate in and killed tumor versus benign human prostate cells. Flow cytometry demonstrated that the mutants are able to infect and replicate in prostate tumor cells (22Rv1), resulting in effective cell killing. However, these mutants showed highly restricted spread in benign prostatic hyperplasia cells (BPH-1). Upon further exploration, it was determined that the restriction observed in the BPH-1 cells is due to the induction and signaling of type-I Interferon (IFN). This was confirmed upon treatment with an IFN-? neutralizing antibody, which relieved restricted spread of mutants in benign cells. BPH-1 cells infected with the mutants also showed upregulation of key anti-viral, IFN-induced genes such as TLR3, IFIT1, and OAS2. Upon characterization of the mutant viruses in an additional metastatic prostate cancer cell line (C4-2B), a restriction in viral spread was observed. The restricted spread did not correlate with production of high levels of type-I IFN, suggesting that other cytokines or intracellular factors can limit replication in tumor cells. Therefore, these studies lay the groundwork for further improving the specificity of oncolytic PIV5 mutants by exploiting type-I IFN pathways as well as other anti-viral factors.
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Date Issued
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2016
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Identifier
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CFE0006468, ucf:51445
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Format
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Document (PDF)
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PURL
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http://purl.flvc.org/ucf/fd/CFE0006468
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Title
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Impact of Cancer-Specific Advance Care Planning on Anxiety, Decisional Conflict, and Surrogate Understanding of Patient Treatment Preferences.
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Creator
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Waser, Lynn, Aroian, Karen, Chase, Susan, Norris, Anne, Loerzel, Victoria, Buckey, Julia, University of Central Florida
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Abstract / Description
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ABSTRACTPatients with life-limiting cancer and their families face unique challenges that interfere with their ability to make decisions or adequately express their health care preferences about end of life (EOL) treatment. As a result, patients at EOL often receive aggressive unwanted treatment that nationally costs billions of dollars and results in surrogate distress about not honoring patient wishes. Respecting Choices(&)#174; DS-ACP is a disease-specific Advance Care Planning (ACP)...
Show moreABSTRACTPatients with life-limiting cancer and their families face unique challenges that interfere with their ability to make decisions or adequately express their health care preferences about end of life (EOL) treatment. As a result, patients at EOL often receive aggressive unwanted treatment that nationally costs billions of dollars and results in surrogate distress about not honoring patient wishes. Respecting Choices(&)#174; DS-ACP is a disease-specific Advance Care Planning (ACP) intervention that is designed to overcome barriers associated with ACP and potentially decrease the incidence of unwanted, overly aggressive treatments at EOL. The intervention is delivered to patient-surrogate dyads by a trained facilitator who provides an opportunity for patients to identify values and goals that support their EOL choices and communicate these values and goals to their surrogates before they are in a medical crisis. Although Respecting Choices(&)#174; DS-ACP has been effective with other populations, it has not been evaluated for patients with life-limiting cancer. Thus, the purpose of this study was to evaluate the Respecting Choices(&)#174; DS-ACP intervention with patients with life-limiting cancer to determine if the intervention increases patient-surrogate congruence about the patient's EOL wishes and reduces decisional conflict without causing anxiety.Study design was a Phase I clinical trial. A volunteer sample of 15 patients with a diagnosis of life limiting cancer and their matched surrogates participated in the study. The Statement of Treatment Preferences for Life-Limiting Cancer Form, the Spielberger State-anxiety Scale Form Y-1 (STAI) and the Decisional Conflict Scale (DCS) were administered pre- and post-intervention. The Quality of Communication about End of Life Care Form was administered at post test. Descriptive statistics were used to describe the sample. McNemar Chi-square and Binomial tests were conducted to investigate whether the intervention increased congruence for five different situations on the Statement of Treatment Preferences for Life-Limiting Cancer Form. The Zar's Multiple Comparison Test of Differences was conducted to investigate the proportion of congruence observed across the five situations. A paired-sample t test was conducted to evaluate post-intervention changes in anxiety (STAI) and decisional conflict (DCS). Frequencies and percentages were conducted for the five items on the Quality of Communication about End of Life Care Form to evaluate patients' and surrogates' satisfaction with the intervention. Anecdotal comments about timing were content analyzed and summarized.Congruence between patients and surrogates improved significantly in all five situations (range of p =.001 to .031), decisional conflict lessened significantly (t (14) =4.49, p (<) .001), and anxiety did not change (t (14) = 1.75, p = .102) pre- and post-intervention. Participants reported satisfaction with the intervention, including its delivery and timing.Findings from this study provide guidance on how to assist patients with life limiting cancer and their surrogates with EOL decision making. Study findings also support making the Respecting Choices ACP intervention part of usual care for patients with life limiting cancer and timing the intervention so that it is delivered before a medical crisis occurs. The lack of change in post-intervention anxiety scores suggests that ACP does not add to patient distress when ACP is conducted by a trained facilitator. This finding can be used to persuade health professionals to refer their patients for ACP. Additional research is needed to determine if increased patient-surrogate congruence leads to patients' wishes being followed and reduces surrogate decisional conflict and distress at EOL. Future research is also needed to determine if the Respecting Choices DS-ACP intervention is equally effective with racial and ethnic groups whose reluctance to engage in EOL discussion has been documented in the literature or if the intervention needs to be culturally adapted.
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Date Issued
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2012
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Identifier
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CFE0004615, ucf:49944
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Format
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Document (PDF)
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PURL
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http://purl.flvc.org/ucf/fd/CFE0004615
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Title
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Chaperonin Containing TCP1 (CCT) as a Target for Cancer Therapy.
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Creator
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Carr, Ana, Khaled, Annette, Altomare, Deborah, Tigno-Aranjuez, Justine, Fernandez-Valle, Cristina, University of Central Florida
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Abstract / Description
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Treatments for aggressive cancers like triple negative breast cancer (TNBC) and small-cell lung cancer (SCLC) have not improved and remain associated with debilitating side effects. There is an unmet medical need for better, druggable targets and improved therapeutics. To this end, we investigated the role of Chaperonin-Containing TCP1 (CCT), an evolutionarily conserved protein-folding complex composed of eight subunits (CCT1-8), in oncogenesis. Our laboratory was the first to report that the...
Show moreTreatments for aggressive cancers like triple negative breast cancer (TNBC) and small-cell lung cancer (SCLC) have not improved and remain associated with debilitating side effects. There is an unmet medical need for better, druggable targets and improved therapeutics. To this end, we investigated the role of Chaperonin-Containing TCP1 (CCT), an evolutionarily conserved protein-folding complex composed of eight subunits (CCT1-8), in oncogenesis. Our laboratory was the first to report that the CCT2 subunit is highly expressed in breast cancer and could be therapeutically targeted. To determine whether CCT is a marker of disease progression in other cancers, we analyzed CCT2 gene expression in liver, prostate and lung cancer, using publicly available genetic databases, and confirmed findings by assessing CCT2 and client proteins, like STAT3, in tumor tissues by immunohistochemistry. We found that CCT2 was high in all cancers, especially SCLC, and correlated with decreased patient survival. We tested CT20p, the peptide therapeutic developed by our laboratory to inhibit CCT, on SCLC and primary lung cells, finding that CT20p was only cytotoxic to SCLC cells. Since SCLC currently lacks targeted therapeutics, our work yielded a new targeted agent that could improve lung cancer mortality. To establish a mechanism of action for CT20p, we partially knocked out CCT2 in TNBC cells, which decreased tumorigenicity in mice and reduced levels of essential proteins like STAT3. To confirm, we overexpressed CCT2 in non-tumorigenic cells and conferred tumor-like characteristics such as increased migration and elevated STAT3. These studies positioned us to develop and validate a strategy for discovery of new small molecule inhibitors of CCT. We thus advanced the field of cancer research by demonstrating that CCT could have diagnostic potential for cancers, such as SCLC and TNBC, that are a significant cause of human death and showed that targeting CCT is a promising therapeutic approach.
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Date Issued
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2017
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Identifier
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CFE0007280, ucf:52191
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Format
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Document (PDF)
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PURL
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http://purl.flvc.org/ucf/fd/CFE0007280
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Title
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EXPLORING THE EXPERIENCES OF LEARNING MATHEMATICS FOR A CHILD WITH CANCER: A CASE STUDY.
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Creator
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Bello, Elizabeth M, Nickels, Megan, University of Central Florida
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Abstract / Description
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In this research report, I utilize interpretative phenomenological analysis (Smith, Flowers, & Larkin, 2009) to examine the mathematics education experiences of a child with cancer. Two qualitative interviews with a 13-year-old male patient with Hodgkin's Lymphoma and his mother were analyzed. Findings revealed several storylines or themes: living with cancer, environmental barriers, and mathematics in virtual school. Grade level mathematics, content knowledge, and delivery during treatment...
Show moreIn this research report, I utilize interpretative phenomenological analysis (Smith, Flowers, & Larkin, 2009) to examine the mathematics education experiences of a child with cancer. Two qualitative interviews with a 13-year-old male patient with Hodgkin's Lymphoma and his mother were analyzed. Findings revealed several storylines or themes: living with cancer, environmental barriers, and mathematics in virtual school. Grade level mathematics, content knowledge, and delivery during treatment in comparison to the child's healthy peers are also discussed.
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Date Issued
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2017
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Identifier
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CFH2000250, ucf:46003
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Format
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Document (PDF)
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PURL
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http://purl.flvc.org/ucf/fd/CFH2000250
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Title
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MECHANISM OF ACTION AND REGULATION OF MEMBRANE SERINE PROTEASE PROSTASIN IN THE PROSTATE AND PROSTATE CANCER.
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Creator
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Chen, Mengqian, Chai, Karl, University of Central Florida
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Abstract / Description
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The glycosylphosphatidylinositol (GPI)-anchored serine protease prostasin (PRSS8) is expressed at the apical membrane surface of epithelial cells and acts as a suppressor of tumor invasion when re-expressed in highly invasive human prostate and breast cancer cell lines. To better understand the molecular mechanisms underlying the anti-invasion phenotype associated with prostasin re-expression in prostate cancer cells, we expressed wild-type human prostasin or a serine active-site mutant...
Show moreThe glycosylphosphatidylinositol (GPI)-anchored serine protease prostasin (PRSS8) is expressed at the apical membrane surface of epithelial cells and acts as a suppressor of tumor invasion when re-expressed in highly invasive human prostate and breast cancer cell lines. To better understand the molecular mechanisms underlying the anti-invasion phenotype associated with prostasin re-expression in prostate cancer cells, we expressed wild-type human prostasin or a serine active-site mutant prostasin in the PC-3 human prostate carcinoma cells. Molecular changes were measured at the mRNA and the protein levels. The expression of several invasion-promoting molecules is regulated by prostasin re-expression, mediated by a protein-level down-regulation of the epidermal growth factor receptor (EGFR). As a result, the cellular response to EGF was reduced as shown by the down-regulation of EGF-stimulated Erk1/2 phosphorylation. The expression of Slug, urokinase-type plasminogen activator (uPA), urokinase-type plasminogen activator receptor (uPAR), inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), and granulocyte-macrophage colony stimulating factor (GM-CSF) was also down-regulated by prostasin re-expression in the PC-3 cells. Co-expression of prostasin and its activating protease matriptase with EGFR in FT-293 cells induces an apparent proteolytic cleavage of the EGFR in the extracellular domain at two specific sites, generating two N-terminally truncated EGFR fragments, named EGFR135 and EGFR110. The EGFR110 is constitutively tyrosine-phosphorylated, and in its presence the phosphorylation of downstream signaling molecules including Erk1/2 and Akt is increased under serum-free conditions. Neither EGFR135 nor EGFR110 is responsive to EGF stimulation. Deletions of the EGFR extracellular domain (ECD) were generated to map the matriptase-prostasin cleavage sites. Two candidate sites were localized to regions AA1-273 and AA273-410. These data support a mechanism of action for the matriptase-prostasin epithelial extracellular serine protease activation cascade by proteolytically modulating the EGF-EGFR signaling. Prostasin gene expression is down-regulated in high-grade and hormone-refractory prostate cancers. We investigated the mechanisms by which androgens regulate prostasin expression in the prostate and prostate cancer. We treated the LNCaP human prostate cancer cells with dihydrotestosterone (DHT) and measured the mRNA expression of prostasin and potential transcription regulators of prostasin predicted by interrogation of the prostasin gene promoter sequence. Prostasin mRNA expression in the LNCaP cells was not responsive to DHT treatment. DHT marginally up-regulated mRNA expression of SREBP-1c, SREBP-2, and SNAIL, but not SREBP-1a, while dramatically increased SLUG mRNA expression, in a dose-dependent manner. Co-transfection of a prostasin promoter-reporter and SREBP cDNA in HEK-293 cells resulted in stimulation of the promoter activity at ~2 fold by SREBP-1c, and up to 6 fold by SREBP-2; while co-transfection with SNAIL or SLUG cDNA resulted in repression of the promoter activity to 43% or 59%, respectively. Co-transfection of the SLUG cDNA negated SREBP-2 s stimulation of the prostasin promoter in a dose-dependent manner. Transfection of an SREBP-2 cDNA in HEK-293 and DU-145 cells resulted in up-regulation of the endogenously expressed prostasin while transfection of a SLUG cDNA in the LNCaP cells repressed prostasin expression. Multiple SREBP-2 binding sites, known as sterol regulatory elements (SRE s), were identified at positions -897, -538, +8, +71, and +98 (named SRE-897, SRE-538, SRE+8, SRE+71, and SRE+98) in the human prostasin gene promoter. Mutagenesis of the five SRE s was carried out to evaluate their roles in SREBP-2 up-regulation of prostasin. SRE+98, a novel functional sterol regulatory element was found to be the major site for the stimulatory response of prostasin gene expression to SREBP-2. CONCLUSIONS: Prostasin regulates the expression of several invasion-promoting molecules in prostate cancer cells by down-modulating the EGF-EGFR signaling pathway. Active prostasin induces proteolytic cleavage in the EGFR ECD at two specific sites. One of the N-terminally truncated EGFR, the EGFR110 is auto-phosphorylated along with increased phosphorylation of downstream signaling molecules. The effect of the androgen DHT on prostasin expression in prostate cells is mediated via SREBP s, which stimulate the promoter, and Slug, which represses the promoter. Slug is up-regulated by DHT and EGF, providing a molecular mechanism by which epithelial cell-specific genes are silenced during prostate cancer development and progression.
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Date Issued
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2007
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Identifier
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CFE0001782, ucf:47257
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Format
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Document (PDF)
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PURL
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http://purl.flvc.org/ucf/fd/CFE0001782
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Title
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EXPLORING THE RELATIONSHIP BETWEEN SYMPTOM MANAGEMENT AND DISTRESS IN PEDIATRIC ONCOLOGY NURSES.
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Creator
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Schultz, Amanda M, Loerzel, Victoria, University of Central Florida
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Abstract / Description
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Pediatric oncology is known to be a stressful work environment due to the difficult aspects regarding patient care. This known stress related to work and caring for pediatric oncology patients can negatively impact nurses, patients, and families. The purpose of this study is to examine: relationships between patient symptom management and nurse distress; strategies used by nurses to manage symptoms in pediatric patients with cancer; nurse perceptions of the effectiveness of non-pharmacologic...
Show morePediatric oncology is known to be a stressful work environment due to the difficult aspects regarding patient care. This known stress related to work and caring for pediatric oncology patients can negatively impact nurses, patients, and families. The purpose of this study is to examine: relationships between patient symptom management and nurse distress; strategies used by nurses to manage symptoms in pediatric patients with cancer; nurse perceptions of the effectiveness of non-pharmacologic or nursing interventions; and nurse distress related to managing symptoms in pediatric patients with cancer. Registered nurses (N=13) at a local children's hospital participated in an online survey. The survey included the Nurses' Distress and Interventions for Symptoms Survey (NDISS) and the Stressor Scale for Pediatric Oncology Nurses (SSPON). Descriptive and correlation statistics were used to analyze data. Results showed that the most commonly managed symptoms were pain (100%), nausea/vomiting (100%), hair loss (100%), fatigue (92.3%), worry (92.3%), mouth sores (84.6%), and trouble sleeping (69.2%). On average, participants reported using at least 10 strategies to manage these symptoms. The most common strategies included: active listening, encouraging family involvement, family support, and reducing sleep interruptions. Most participants felt like they managed the symptoms effectively. Overall, the most common stressors for pediatric oncology nurses were related to co-workers (71.8%) and system demands (68.9%). There was no statistically significant relationship between symptom management and nurse distress. Further research should be conducted on the relationship between nurses and significant stressors other than symptom management. Identifying these significant stressors, especially related to co-workers and system demands, would be the first step in the development of appropriate interventions, such as supportive programs, for decreasing nurses' stress response.
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Date Issued
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2017
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Identifier
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CFH2000154, ucf:45987
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Format
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Document (PDF)
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PURL
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http://purl.flvc.org/ucf/fd/CFH2000154
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Title
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ONLINE SUPPORT GROUP FOR CHINESE WOMEN WITH OVARIAN OR CERVICAL CANCER.
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Creator
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Xing, Yuan, Loerzel, Victoria, University of Central Florida
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Abstract / Description
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Women with gynecological (GYN) cancer experience a wide spectrum of physical, emotional and social effects after diagnosis and treatment of their cancer. The insufficient availability of local support groups, limited transportation for the rural Chinese population and a shortage of oncologists make it difficult to have face-to-face support groups for Chinese GYN cancer patients. However, the wide access of Internet has provided an opportunity for people to have online support groups. The...
Show moreWomen with gynecological (GYN) cancer experience a wide spectrum of physical, emotional and social effects after diagnosis and treatment of their cancer. The insufficient availability of local support groups, limited transportation for the rural Chinese population and a shortage of oncologists make it difficult to have face-to-face support groups for Chinese GYN cancer patients. However, the wide access of Internet has provided an opportunity for people to have online support groups. The purpose of this study was to observe and describe the types of support given to and by Chinese GYN cancer survivors in a QQ chat group. This was a qualitative study that used the directed content analysis approach. A QQ group was observed for two weeks between March 10th (12:01am) and March 24st (11:59pm) 2018 Beijing time. Observed online posts were copied and pasted into a WORD ™ document for analysis. There were 4 themes observed: sharing experience, information exchange, emotional support and Guardian Against Cancer group member benefits. The results of the study suggested that women supported each other but little evidence-based support was observed. Healthcare providers should be monitoring and engaging in conversations with group members. Well-planed and organized information sessions should also be beneficial for members. Further research on understanding members' needs on online support groups and the effectiveness of intervention should be conducted.
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Date Issued
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2018
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Identifier
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CFH2000382, ucf:45808
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Format
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Document (PDF)
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PURL
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http://purl.flvc.org/ucf/fd/CFH2000382
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Title
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INTERVENTIONS FOR TREATMENT RELATED SIDE EFFECTS IN OLDER WOMEN WITH BREAST CANCER.
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Creator
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Suarez, Stephanie, Loerzel, Victoria, University of Central Florida
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Abstract / Description
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Over half (57%) of the women diagnosed with breast cancer are age 65 and older. Treatment for breast cancer may exacerbate current chronic illnesses and/or cause multiple treatment related side effects such as insomnia, fatigue, decreased physical functioning, alterations in body image, poorer quality of life, and changes in psychosocial health. While many women with breast cancer experience these changes, research suggests that older women have different needs than younger women and may not...
Show moreOver half (57%) of the women diagnosed with breast cancer are age 65 and older. Treatment for breast cancer may exacerbate current chronic illnesses and/or cause multiple treatment related side effects such as insomnia, fatigue, decreased physical functioning, alterations in body image, poorer quality of life, and changes in psychosocial health. While many women with breast cancer experience these changes, research suggests that older women have different needs than younger women and may not always benefit from interventions. The purpose of this integrative review of literature was to evaluate interventions designed to improve treatment related side effects in female breast cancer survivors age 65 years and older. This review of literature was conducted using CINAHL, PsycINFO, and MEDLINE databases using various key terms. Inclusion criteria consisted of peer reviewed research articles, women who have experience breast cancer, interventions directed at decreasing side effects, and research articles written in the English language.While using these search criteria, no interventions were found therefore, the age group was lowered to include women 50 and older. Eleven studies met the inclusion criteria. Interventions addressed a variety of treatment related side effects and were delivered in multiple formats. The findings indicate that interventions resulted in a significant improvement in sleep and fatigue, physical function, perception of body image, psychosocial health, and quality of life for older women with breast cancer. While these findings are positive, the literature did not break down results based on developmental stage or "older" age groups. Currently, there is limited literature that examines interventions in women age 65 and older, this may limit nurses' ability to suggest successful interventions to some of our oldest cancer survivors.
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Date Issued
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2013
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Identifier
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CFH0004393, ucf:44990
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Format
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Document (PDF)
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PURL
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http://purl.flvc.org/ucf/fd/CFH0004393
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Title
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Zika virus-induced lysis of cervical cancer cells.
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Creator
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Krishnapura, Harini, Alexander, Kenneth, Parks, Griffith, Jewett, Mollie, University of Central Florida
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Abstract / Description
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Cervical cancer is the fourth most frequent cancer in women with an estimated 570,000 new cases globally in 2018. Treatment of advanced cervical cancer is often unsuccessful leading to high cancer-related mortality rates, especially in under-resourced countries. Recently, a possible role for the cell surface glycoprotein CD24 in host cell specificity of Zika virus was reported. As an extension of this work, Zika viruses have been proposed as oncolytic therapy for the treatment of...
Show moreCervical cancer is the fourth most frequent cancer in women with an estimated 570,000 new cases globally in 2018. Treatment of advanced cervical cancer is often unsuccessful leading to high cancer-related mortality rates, especially in under-resourced countries. Recently, a possible role for the cell surface glycoprotein CD24 in host cell specificity of Zika virus was reported. As an extension of this work, Zika viruses have been proposed as oncolytic therapy for the treatment of neuroblastoma and other CD24 positive tumors. To determine the permissiveness of cervical cancer cells to Zika virus infection and its association with CD24, we assessed cytopathic effect (CPE) induced by Zika virus in cervical cancer cell lines (HeLa, SiHa and CaSki) by light microscopy and by cytotoxicity assay. Cervical cancer cells were susceptible to Zika virus-induced apoptosis. Upon infection, the morphology of cervical cancer cells changed, exhibiting Zika virus-induced CPE. Cervical cancer cell expression of viral non-structural protein 1 (NS1) after infection demonstrated viral protein translation. Quantitative plaque assays demonstrated the production of competent virions. Because CD24 expression was found to be important for Zika virus infection in neuroblastoma cells, CD24 expression was assessed in cervical cancer cells. Cervical cancer cells expressed low but measurable levels of CD24 mRNA and protein. siRNA-mediated knockdown of CD24 resulted in reduced NS1 expression and reduced levels of virus-induced apoptosis. Taken together our data suggest a possible role for CD24 in Zika virus-induced apoptosis in cervical cancer cells. Zika virus-induced apoptosis of cultured cervical cancer cells presents the possibility for the use of Zika virus as a potential oncolytic therapy for cervical cancer.
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Date Issued
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2019
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Identifier
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CFE0007480, ucf:52682
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Format
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Document (PDF)
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PURL
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http://purl.flvc.org/ucf/fd/CFE0007480
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Title
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Fabrication of Polyelectrolyte Nanoparticles Through Hydrophobic Interaction.
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Creator
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Catarata, Ruginn Porce, Zhai, Lei, Kang, Ellen, Huo, Qun, University of Central Florida
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Abstract / Description
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Anticancer drugs like gemcitabine (GEM) are used to treat cancers such as, pancreatic ductal adenocarcinoma (PDAC). However, the use of free gemcitabine yields challenges including cytotoxicity to healthy cells and poor circulation time. By encapsulating GEM in nanoparticles these challenges can be overcome. In this study poly(acrylic acid) (PAA)-GEM nanoparticles are fabricated by coupling GEM onto PAA. The particle formation is driven by the hydrophobic interaction of GEM, which collects in...
Show moreAnticancer drugs like gemcitabine (GEM) are used to treat cancers such as, pancreatic ductal adenocarcinoma (PDAC). However, the use of free gemcitabine yields challenges including cytotoxicity to healthy cells and poor circulation time. By encapsulating GEM in nanoparticles these challenges can be overcome. In this study poly(acrylic acid) (PAA)-GEM nanoparticles are fabricated by coupling GEM onto PAA. The particle formation is driven by the hydrophobic interaction of GEM, which collects in the core of the nanoparticle, forming a PAA shell. The nanoparticles were optimized by studying the PAA/GEM ratio and pH during fabrication. Characteristics of the nanoparticles including size, morphology and surface charge were investigated using dynamic light scattering (DLS), transmission electron microscopy (TEM) and zeta potential measurements. Conditions such as ionic stability and pH stability were optimized to achieve high drug loading efficiency. Cell uptake and cytotoxicity studies were used to determine the efficiency of the nanoparticles as drug delivery vehicle.
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Date Issued
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2019
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Identifier
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CFE0007791, ucf:52364
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Format
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Document (PDF)
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PURL
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http://purl.flvc.org/ucf/fd/CFE0007791
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Title
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Telephone-Delivered Cognitive Behavioral Therapy for Insomnia in Patients with Cancer: A Randomized Controlled Trial.
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Creator
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Nicasio, Andel, Blaney, Cerissa, Robinson, Diane, Bedwell, Jeffrey, Modianos, Doan, Robinson, Diane, University of Central Florida
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Abstract / Description
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This study examined the efficacy and feasibility of a brief telephone-delivered CBT-I (TeleCBT-I) intervention in cancer patients compared to a control group. The study used a randomized controlled trial design. The TeleCBT-I program consisted of a brief four-week CBT-I program adapted for cancer patients. Patients completed assessment measures at pre-treatment, post-treatment and one-month follow-up. Out of 184 patients screened, 39 were randomly assigned, and 35 (TeleCBT-I, n = 19; Control,...
Show moreThis study examined the efficacy and feasibility of a brief telephone-delivered CBT-I (TeleCBT-I) intervention in cancer patients compared to a control group. The study used a randomized controlled trial design. The TeleCBT-I program consisted of a brief four-week CBT-I program adapted for cancer patients. Patients completed assessment measures at pre-treatment, post-treatment and one-month follow-up. Out of 184 patients screened, 39 were randomly assigned, and 35 (TeleCBT-I, n = 19; Control, n = 16) completed pre- and post-treatment measures and were included in the analyses. Compared to control group, the TeleCBT-I group reported decreased insomnia severity symptoms (p (<) .014), improved sleep quality (p (<) .023), and reduced dysfunctional beliefs about sleep (p = .039) at post-treatment with sustained treatment effects at one-month follow-up. Sleep measures yielded large effect sizes (Hedges' g, 0.84-2.7). Although the TeleCBT-I group indicated improvements in fatigue, general functioning, physical well-being, functional well-being, and physical quality of life, effects at follow-up were observed only for fatigue, functional well-being and physical quality of life. No effects were found on depression at any of the time points. In terms of feasibility, TeleCBT-I demonstrated high adherence, high homework completion and high overall satisfaction. These results advance the empirical evidence of CBT-I in cancer patients and support the use of telephone-delivered CBT-I to widely disseminate and implement among patients with cancer.
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Date Issued
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2019
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Identifier
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CFE0007694, ucf:52439
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Format
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Document (PDF)
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PURL
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http://purl.flvc.org/ucf/fd/CFE0007694
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Title
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SYNTHESIS OF NOVEL AZIRIDINE DERIVATIVES OF PODOCARPIC ACID.
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Creator
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Rhoden, Stephen, Miles, Howard, University of Central Florida
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Abstract / Description
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Podocarpic acid (a diterpenoid resin acid extracted from the Podocarpacea specie of plants) has shown cytotoxicity against carcinoma of the nasopharynx. Since this discovery has been made, research has been performed in order to alter the structure of the resin acid so as to increase the anticancer activity. The carboxylic acid and phenol functional groups, which are present in podocarpic acid, make it possible to synthesize new derivatives selectively at the C-15, C-13, and C-7 positions as...
Show morePodocarpic acid (a diterpenoid resin acid extracted from the Podocarpacea specie of plants) has shown cytotoxicity against carcinoma of the nasopharynx. Since this discovery has been made, research has been performed in order to alter the structure of the resin acid so as to increase the anticancer activity. The carboxylic acid and phenol functional groups, which are present in podocarpic acid, make it possible to synthesize new derivatives selectively at the C-15, C-13, and C-7 positions as well as by substitution of the phenol hydroxyl group. Thus numerous derivatives can be prepared, in high yield, for the purpose of investigating their potential, as new drug leads for the treatment of cancer. In this study, Doyle's catalyst (Dirhodium tetrakis caprolactamate) was used to form a novel derivative in high yield (85%) which contained a 3-membered aziridine ring at the C-6 and C-7 position. The main thrust of this research involved the formation a series of novel derivatives of the aziridine compound by utilizing phenol and m-chlorophenol as nucleophiles to open the aziridine ring. These novel compounds will now be sent to the National Institute of Health (NIH) for bioassay against 60 human cancer cell lines.
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Date Issued
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2007
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Identifier
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CFE0001759, ucf:52849
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Format
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Document (PDF)
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PURL
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http://purl.flvc.org/ucf/fd/CFE0001759
Pages