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Title: COARSE GRAINED MONTE CARLO SIMULATION OF THE SELF-ASSEMBLY OF THEHIV-1 CAPSID PROTEIN.
Creator: Weber, Jeffrey, Chen, Bo, University of Central Florida
Abstract / Description: In this study, a Monte Carlo simulation was designed to observe the self-assembly of the HIV-1 capsid protein. The simulation allowed a coarse grained model of the capsid protein with defined interaction sites to move freely in three dimensions using the Metropolis criterion. Observations were made as to which parameters affected the assembly the process. The ways in which the assembly were affected were also noted. It was found that proper dimerization of the capsid protein was necessary in...
Show moreIn this study, a Monte Carlo simulation was designed to observe the self-assembly of the HIV-1 capsid protein. The simulation allowed a coarse grained model of the capsid protein with defined interaction sites to move freely in three dimensions using the Metropolis criterion. Observations were made as to which parameters affected the assembly the process. The ways in which the assembly were affected were also noted. It was found that proper dimerization of the capsid protein was necessary in order for the lattice to form properly. It was also found that a strong trimeric interface could be responsible for double-layered assemblies. Further studies may be conducted by further varying of parameters or reworking the dynamics of the simulation. The possible causes of curvature within the assembly still need to be researched further.
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Date Issued: 2014
Identifier: CFH0004618, ucf:45316
Format: Document (PDF)
PURL: http://purl.flvc.org/ucf/fd/CFH0004618

Title: HOMOPHOBIA AND HIV TRANSMISSION: A SIX COUNTRY COMPARATIVE ANALYSIS.
Creator: Middleton, Tiernan, Mishtal, Joanna, University of Central Florida
Abstract / Description: This interdisciplinary study combines epidemiological data with anthropological theory to investigate the relationship between HIV transmission rates and systemic homophobia. Previous research has illustrated the link between high levels of structural violence and structural stigma to increased risk of diseases such as the link between African-Americans and heart disease. This study investigates the relationship between systemic homophobia and HIV transmission rates. Through operationalizing...
Show moreThis interdisciplinary study combines epidemiological data with anthropological theory to investigate the relationship between HIV transmission rates and systemic homophobia. Previous research has illustrated the link between high levels of structural violence and structural stigma to increased risk of diseases such as the link between African-Americans and heart disease. This study investigates the relationship between systemic homophobia and HIV transmission rates. Through operationalizing homophobia into seven distinct factors, I evaluated systemic homophobia in six countries, assigning a score 1-10 to each factor using secondary source aggregation. I compared composite scores, as well as scores in each operationalized factor to HIV transmission rates in those countries. The results of this study indicate a correlation between systemic homophobia and increased HIV transmission, particularly in respect to the factors Marriage Equality, LGBT Laws, Religiosity, LGBT Visibility, and Hate Crimes. Though various sociocultural factors play a role in HIV transmission, this study indicates that homophobia plays an integral role in HIV transmission. This project has pertinent applications in epidemiology, anthropology and public health illustrating the integral role of sociocultural and systemic factors that increase structural violence and risk for a disease.
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Date Issued: 2015
Identifier: CFH0004814, ucf:45458
Format: Document (PDF)
PURL: http://purl.flvc.org/ucf/fd/CFH0004814

Title: Exploring the influence of stigma, level of trauma, and social support on the experience of posttraumatic growth in adults living with HIV.
Creator: Zeligman, Melissa, Hagedorn, William, Barden, Sejal, Hundley, Gulnora, Xu, Lihua, University of Central Florida
Abstract / Description: The present study investigated the influence of HIV-related stigma, social support, and impact of HIV diagnosis on posttraumatic growth (PTG) in adults living with HIV (N = 126). In addition, the study aimed to identify if social support moderated the relationship between stigma and PTG. Lastly, the study attempted to determine how impactful receiving an HIV diagnosis was to the sample. One hundred and twenty-six adults living with HIV within the state of Florida (41% response rate)...
Show moreThe present study investigated the influence of HIV-related stigma, social support, and impact of HIV diagnosis on posttraumatic growth (PTG) in adults living with HIV (N = 126). In addition, the study aimed to identify if social support moderated the relationship between stigma and PTG. Lastly, the study attempted to determine how impactful receiving an HIV diagnosis was to the sample. One hundred and twenty-six adults living with HIV within the state of Florida (41% response rate) participated in the research. Participants were recruited from a series of support groups and HIV focused agencies throughout the state, and responded through face to face survey administration. Each assessment packet consisted of the following assessments: (a) Posttraumatic Growth Inventory; [PTGI], Tedeschi (&) Calhoun, 1996, (b) Multidimensional Scale of Perceived Social Support; [MSPSS], Zimet, Dahlem, Zimet, (&) Farley, 1988, (c) Berger HIV Stigma Scale; Berger, Ferrans, (&) Lashley, 2001, (d) Impact of Event Scale [IES-R . Weiss (&) Marmar, 1996], (e) Reynolds Short Form Social Desirability Scale; Reynolds, 1982, and (f) a demographic questionnaire. Multiple regression analysis was used to determine whether the independent variables were predictive of the outcome of PTG. Findings from the study revealed that each of the predictor variables (i.e., stigma, social support, and impact of diagnosis) contributed significantly (p(<).05) to the model, and accounted for 12% of the variance in PTGI scores. In examining the moderating presence of social support between stigma and PTG, there was no found significant interaction between stigma and social support. Significant differences in PTG scores were also identified amongst differing demographic groups, specifically participant ethnicity and religious orientation. Findings also revealed that 38.5% of the sample reported their HIV diagnosis as so impactful that they would likely qualify for a clinical diagnosis of posttraumatic stress disorder (PTSD). Furthermore, a detailed discussion of previous literature, study procedures and methodology, counselor implications, counselor educator implications, and recommendations for future research are discussed.
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Date Issued: 2014
Identifier: CFE0005285, ucf:50563
Format: Document (PDF)
PURL: http://purl.flvc.org/ucf/fd/CFE0005285

Title: MIGRANT LABOR, DEVELOPMENT, AND HIV IN BOTSWANA.
Creator: Kearns, William, Walker, Ezekiel, University of Central Florida
Abstract / Description: At independence, Botswana was highly underdeveloped and reliant on external capital earned through migrant labor. This presented several challenges to development despite the discovery of diamonds shortly after independence. However, no challenge was greater than the HIV epidemic which came to infect one in four Batswana. This thesis discusses the historical factors which promoted the spread of the virus in the greater context of migrant labor and development within Botswana.
Date Issued: 2014
Identifier: CFH0004718, ucf:45354
Format: Document (PDF)
PURL: http://purl.flvc.org/ucf/fd/CFH0004718

Title: A meta-analytic approach to examining psychosocial correlates of risk in HIV-diagnosed and HIV-nondiagnosed men who have sex with men.
Creator: Lacefield, Katharine, Negy, Charles, Beidel, Deborah, Dunn, Stacey, University of Central Florida
Abstract / Description: Human Immunodeficiency Virus (HIV) continues to disproportionately affect men who have sex with men (MSM). Gay, bisexual, and other MSM are estimated to account for two percent of the population, yet they constitute more than half of all individuals living with HIV in the United States (Centers for Disease Control [CDC], 2010). Collectively, both HIV-diagnosed (HIV-D) and HIV-nondiagnosed (HIV-ND) MSM report a variety of reasons for intentional and unintentional nonuse of condoms. Depending...
Show moreHuman Immunodeficiency Virus (HIV) continues to disproportionately affect men who have sex with men (MSM). Gay, bisexual, and other MSM are estimated to account for two percent of the population, yet they constitute more than half of all individuals living with HIV in the United States (Centers for Disease Control [CDC], 2010). Collectively, both HIV-diagnosed (HIV-D) and HIV-nondiagnosed (HIV-ND) MSM report a variety of reasons for intentional and unintentional nonuse of condoms. Depending on partner status, HIV-D MSM are in the unique position of having the potential both to increase risk of infecting others with HIV and to expose themselves to further complication (e.g., superinfection) when they engage in unprotected anal intercourse (UAI). HIV-ND MSM are at risk of seroconversion each time they engage in UAI with an infected partner or partner of unknown HIV status. Elucidating reasons for continued engagement in UAI specific to both HIV-D and HIV-ND MSM likely is an important step in the process of effective prevention. The current study employed meta-analytic methods to evaluate HIV-risk correlates in both HIV-D and HIV-ND MSM. In addition to several individual risk correlates, within the context of the Information-Motivation-Behavioral Skills Model, Behavioral Skills variables were related to condom use in HIV-ND MSM and Motivation variables were related to condom use in both HIV-D and HIV-ND MSM. A sufficient number of studies were not available to examine Information-based risk correlates in either subgroup. Results of the present study may guide future best practice in harm reduction for both HIV-D and HIV-ND MSM.
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Date Issued: 2013
Identifier: CFE0004874, ucf:49647
Format: Document (PDF)
PURL: http://purl.flvc.org/ucf/fd/CFE0004874

Title: THE EFFECT OF BACTERIAL VAGINOSIS-ASSOCIATED BACTERIA ON EPITHELIAL FACTORS MEDIATING HIV TRANSMISSION.
Creator: Nguyen, April, Cole, Alexander, University of Central Florida
Abstract / Description: Bacterial vaginosis (BV), a common female reproductive tract (FRT) condition characterized by an overgrowth of anaerobic species concurrent with the disappearance of commensal Lactobacilli species, is associated with a 60% increased risk of HIV-1 transmission. However, the role of the FRT epithelia in bacterial vaginosis-associated bacteria (BVAB)-augmented HIV-1 transmission is unclear. To evaluate the increased risk of HIV-1 acquisition, we treated FRT epithelia with Atopobium vaginae, a...
Show moreBacterial vaginosis (BV), a common female reproductive tract (FRT) condition characterized by an overgrowth of anaerobic species concurrent with the disappearance of commensal Lactobacilli species, is associated with a 60% increased risk of HIV-1 transmission. However, the role of the FRT epithelia in bacterial vaginosis-associated bacteria (BVAB)-augmented HIV-1 transmission is unclear. To evaluate the increased risk of HIV-1 acquisition, we treated FRT epithelia with Atopobium vaginae, a prevalent BVAB, to determine the nature of the host response to BVAB exposure. Treatment of endocervical cells with A. vaginae resulted in a 1500-fold increase in the expression of the antimicrobial peptide hBD-2, an inflammatory cytokine response, and delocalization of the tight junction protein ZO-1 from cell borders. Conditioned media (CM) from the coculture of FRT epithelia and A. vaginae also generated an inflammatory immune response and lowered the transepithelial electrical resistance in polarized endocervical monolayers. Changes in HIV-1 infection were measured in TZM-bl reporter cells, which contain a luciferase gene under the control of an HIV-1 long terminal repeat (LTR) region that is activated by the binding of Tat, an HIV-1 protein that drives viral replication. NF[kappa]B is a major host-derived transcription factor that regulates the expression of many genes involved in inflammation and the innate immune response. Interestingly, NF[kappa]B has been reported to bind Tat-activated response elements within the LTR of HIV-1, driving viral transcription. TZM-bl cells were treated with CM in the absence of HIV-1, which resulted in increased luciferase production that could be suppressed by the NF[kappa]B inhibitor TPCA-1. These data suggest that epithelially derived products from the coculture of FRT cells and A. vaginae enhance HIV-1 infection by causing cervical barrier dysfunction and increasing HIV replication efficiency through NF[kappa]B.
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Date Issued: 2015
Identifier: CFH0004752, ucf:45365
Format: Document (PDF)
PURL: http://purl.flvc.org/ucf/fd/CFH0004752

Title: DO YOU HAVE A FRIEND?: THE IMPACT OF PERSONAL KNOWLEDGE OF SOMEONE WITH AIDS ON ATTITUDES TOWARDS AIDS.
Creator: Lombas, Leith Leonce, Gay, David, University of Central Florida
Abstract / Description: ABSTRACT The study of attitudes towards those with AIDS is relatively recent. Most studies have examined attitudes concerning health and medical concerns. Little research has focused on attitudes toward social and behavior concerns. The few that have focused on such attitudes have employed relatively small samples collected primarily out of convenience. The studies that have used national samples have primarily addressed public policy issues. Using national data from the 1988 General Social...
Show moreABSTRACT The study of attitudes towards those with AIDS is relatively recent. Most studies have examined attitudes concerning health and medical concerns. Little research has focused on attitudes toward social and behavior concerns. The few that have focused on such attitudes have employed relatively small samples collected primarily out of convenience. The studies that have used national samples have primarily addressed public policy issues. Using national data from the 1988 General Social Survey, this paper examines the effects of personal knowledge about the AIDS virus and other attitudinal variables on four dimensions of social and behavioral concern for those with AIDS in American society. Sociodemographic variables, which prior studies have demonstrated as important predictors of attitudes toward AIDS, are included as controls in this research that presents findings from a multivariate analysis. Results suggests that the impact of personal knowledge of someone with AIDS does not strongly lead to more supportive attitudes regarding the rights of people with AIDS, except when the economic costs of AIDS care is concerned. Directions for future research are presented and discussed.
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Date Issued: 2004
Identifier: CFE0000010, ucf:46071
Format: Document (PDF)
PURL: http://purl.flvc.org/ucf/fd/CFE0000010

Title: RETROCYCLIN RC-101 OVERCOMES CATIONIC MUTATIONS ON THE HEPTAD REPEAT 2 OF HIV-1 GP41.
Creator: Fuhrman, Christopher, Cole, Alexander, University of Central Florida
Abstract / Description: Retrocyclin RC-101, a θ-defensin with lectin-like properties, potently inhibits infection by many HIV-1 subtypes by binding to the heptad repeat (HR)-2 region of gp41 and preventing six-helix bundle formation. In the present study, we used in silico computational exploration to identify residues of HR2 that interacted with RC-101 and then analyzed the HIV-1 Sequence Database at LANL for residue variations in the HR1 and HR2 segments that could plausibly impart in vivo resistance. Docking...
Show moreRetrocyclin RC-101, a θ-defensin with lectin-like properties, potently inhibits infection by many HIV-1 subtypes by binding to the heptad repeat (HR)-2 region of gp41 and preventing six-helix bundle formation. In the present study, we used in silico computational exploration to identify residues of HR2 that interacted with RC-101 and then analyzed the HIV-1 Sequence Database at LANL for residue variations in the HR1 and HR2 segments that could plausibly impart in vivo resistance. Docking RC-101 to gp41 peptides in silico confirmed its strong preference for HR2 over HR1, and implicated residues crucial for its ability to bind HR2. We mutagenized these residues in pseudotyped HIV-1 JR.FL reporter viruses, and subjected them to single round replication assays in the presence of 1.25-10ug/ml RC-101. Except for one mutant that was partially resistant to RC-101, the other pseudotyped viruses with single-site cationic mutations in HR2 manifested absent or impaired infectivity or retained wild-type susceptibility to RC-101. Overall, these data suggest that most mutations capable of rendering HIV-1 resistant to RC-101 will also exert deleterious effects on the ability of HIV-1 to initiate infections - an interesting and novel property for a potential topical microbicide.
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Date Issued: 2007
Identifier: CFE0001707, ucf:47333
Format: Document (PDF)
PURL: http://purl.flvc.org/ucf/fd/CFE0001707

Title: ON MODELING HIV INFECTION OF CD4+ T CELLS.
Creator: Comerford, Amy, Mohapatra, Ram, University of Central Florida
Abstract / Description: We examine an early model for the interaction of HIV with CD4+ T cells in vivo and define possible parameters and effects of said parameters on the model. We then examine a newer, more simplified model for the interaction of HIV with CD4+ T cells that also considers four populations: uninfected T cells, latently infected T cells, actively infected T cells, and free virus. The stability of both the disease free steady state and the endemically infected steady state are examined utilizing...
Show moreWe examine an early model for the interaction of HIV with CD4+ T cells in vivo and define possible parameters and effects of said parameters on the model. We then examine a newer, more simplified model for the interaction of HIV with CD4+ T cells that also considers four populations: uninfected T cells, latently infected T cells, actively infected T cells, and free virus. The stability of both the disease free steady state and the endemically infected steady state are examined utilizing standard methods and the Routh-Hurwitz criteria. We show that if N, the number of infectious virions produced per actively infected T cell, is less than a critical value, , then the uninfected state is the only steady state in the non negative orthant, and this state is stable. We establish an expression for . If , then the uninfected steady state is unstable, and the endemically infected state can be stable or unstable, depending on the value of the parameters utilized.
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Date Issued: 2006
Identifier: CFE0001093, ucf:46769
Format: Document (PDF)
PURL: http://purl.flvc.org/ucf/fd/CFE0001093

Title: WHAT ARE THE MOST COMMONLY USED TOOLS TO SCREEN DEPRESSION IN HIV-INFECTED GAY AND BISEXUAL MEN?.
Creator: Isner, Michael, Blackwell, Christopher, University of Central Florida
Abstract / Description: Gay and bisexual men infected with human immunodeficiency virus (HIV) comprise a sizable, medically vulnerable population. Depression is the most commonly experienced mental health disorder affecting this group of people, lending itself to a host of risks associated with depression. As screening of depression in this population can be challenging, it is vital that clinicians have the best available tools and guidelines to detect depressive symptomology. This focused, comprehensive review of...
Show moreGay and bisexual men infected with human immunodeficiency virus (HIV) comprise a sizable, medically vulnerable population. Depression is the most commonly experienced mental health disorder affecting this group of people, lending itself to a host of risks associated with depression. As screening of depression in this population can be challenging, it is vital that clinicians have the best available tools and guidelines to detect depressive symptomology. This focused, comprehensive review of the literature examined current data describing the clinical instruments used to detect depressive symptoms in HIV-infected gay and bisexual men. The aim of this analysis was to seek out which instruments were the most widely and successfully employed for this population. An initial search using EBSCOhost and associated databases CINAHL, MEDLINE, PsycINFO, and Health and Psychosocial Instruments alongside inclusion and exclusion criteria found 1,899 articles. Results were narrowed using additional inclusion and exclusion criteria and relevancy, yielding a total of 13 articles for review. The findings of this review suggest screening of depressive symptoms in HIV-infected gay and bisexual men was most successful using the CES-D, the BSI-18, and the BDI. Health care providers should have an understanding of the importance in assessing this population for depression and have access to the best possible tools to do so.
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Date Issued: 2017
Identifier: CFH2000205, ucf:45928
Format: Document (PDF)
PURL: http://purl.flvc.org/ucf/fd/CFH2000205

Title: Emotional Labor and Identity Management Among HIV Counselors and Testers.
Creator: Caldwell, James, Anthony, Amanda, Donley, Amy, Gay, David, University of Central Florida
Abstract / Description: Emotional labor, an idea first developed by Arlie Hochschild, became a main component of work developing the field of sociology of emotions. Emotion labor provides a conceptual framework for understanding the outward and inward emotional experiences that are deemed either appropriate or inappropriate during interactions with others, specifically in the workplace. A product and derivation of this emotional labor is carefully outlined display rules. These rules vary from position to position,...
Show moreEmotional labor, an idea first developed by Arlie Hochschild, became a main component of work developing the field of sociology of emotions. Emotion labor provides a conceptual framework for understanding the outward and inward emotional experiences that are deemed either appropriate or inappropriate during interactions with others, specifically in the workplace. A product and derivation of this emotional labor is carefully outlined display rules. These rules vary from position to position, but are often part and parcel of work in the human services sector. This labor can be understood as resulting from the employee's adherence to display rules, which may or may not match the employees' organically felt or perceived emotions at the time. The current study draws from these conceptual frameworks and emotion work typologies introduced by Arlie Hochschild to analyze in-depth, the emotional labor performed by HIV Testers; this study does so through the analytical categories of Bodily Emotion Work, Expressive Emotion Work, and Cognitive Emotion Work. While the current study upholds many conclusions of prior research related to human services, and high rates of emotional labor, this study contributes through introducing the term Emotional Tuning. As based in the dynamic of emotional labor existing between HIV Testers and the patients that they serve, this study puts forth the term Emotional Tuning as the process of one individual scanning or reviewing the emotional state of another. The individual then acts accordingly, based on their interpretation of the other's emotional state, to help influence that emotional state, typically by matching or contrasting with that emotional state. This research contributes by expanding on prior research of emotion work and emotion labor through the specific field of client-based counseling, as there is no known prior research that has delved specifically into the work performed by HIV Testers and the rich experiences had by those delivering HIV results and sexual education, particularly as the emotional labor being studied is not commodified. Such topics as HIV status and sexual health education have been, even recently, overshadowed by stigma. Many Testers in the current study found HIV work to be both the hardest and most rewarding experience of their life. The current study looked closely at the effect that this emotional work had on both the testers interviewed, and the patients they serve and has broad implications for both tester training and client support initiatives.
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Date Issued: 2016
Identifier: CFE0006274, ucf:51036
Format: Document (PDF)
PURL: http://purl.flvc.org/ucf/fd/CFE0006274

Title: Two-Component Covalent Inhibitors (TCCI) of the Human Immunodeficiency Virus Reverse Transcriptase (HIV-RT).
Creator: Ledezma, Carlos, Kolpashchikov, Dmitry, Yestrebsky, Cherie, Hernandez, Florencio, Zhai, Lei, Tatulian, Suren, University of Central Florida
Abstract / Description: The traditional design of nucleoside reverse transcriptase inhibitors (NRTI's) involves the synthesis of chain-terminated nucleoside analogs. HIV-RT has relatively low fidelity which facilitates mutations that confer resistance towards NRTI's, also, drug promiscuity from NRTI's result in various side-effects that lead to poor patient adherence to treatment. We designed and tested two-component covalent inhibitors against HIV-RT. Our inhibitor design results in higher specificity due to its...
Show moreThe traditional design of nucleoside reverse transcriptase inhibitors (NRTI's) involves the synthesis of chain-terminated nucleoside analogs. HIV-RT has relatively low fidelity which facilitates mutations that confer resistance towards NRTI's, also, drug promiscuity from NRTI's result in various side-effects that lead to poor patient adherence to treatment. We designed and tested two-component covalent inhibitors against HIV-RT. Our inhibitor design results in higher specificity due to its binary approach, which has previously been used in biosensing applications, where both components are necessary for therapeutic effect, and lower chances for mutagenesis because of its inhibitory action. The TCCI approach results in up to 93% inhibition of HIV-RT Furthermore, our inhibitor design is highly modular and can be adjusted towards the therapeutic targeting of other biopolymers.
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Date Issued: 2017
Identifier: CFE0006893, ucf:51712
Format: Document (PDF)
PURL: http://purl.flvc.org/ucf/fd/CFE0006893

Title: Antimicrobial Peptide Resistance and Immunomodulation by HIV-1 gp41.
Creator: Wood, Matthew, Cole, Alexander, Chai, Karl, Teter, Kenneth, Parkinson, Christopher, University of Central Florida
Abstract / Description: Fusion inhibitors are a class of antiretroviral drugs used to prevent entry of HIV into host cells. Many of the fusion inhibitors being developed, including the drug enfuvirtide (ENF), are peptides designed to mimic, and thereby competitively inhibit, the viral fusion protein gp41. An exception to this is a class of cyclic, cationic, antimicrobial peptides known as ?-defensins, which are produced by many non-human primates and exhibit broad-spectrum antiviral and antibacterial activity....
Show moreFusion inhibitors are a class of antiretroviral drugs used to prevent entry of HIV into host cells. Many of the fusion inhibitors being developed, including the drug enfuvirtide (ENF), are peptides designed to mimic, and thereby competitively inhibit, the viral fusion protein gp41. An exception to this is a class of cyclic, cationic, antimicrobial peptides known as ?-defensins, which are produced by many non-human primates and exhibit broad-spectrum antiviral and antibacterial activity. Currently, the ?-defensin analog RC-101 is being developed as a microbicide to prevent sexual transmission of HIV-1. Understanding potential RC-101 resistance, and how resistance to other fusion inhibitors affects RC-101 susceptibility, is critical for future development.Partial drug resistance due to genetic variability within HIV-1 presents a major hurdle in microbicide development. Drug-resistance mutations, whether naturally occurring or resulting from selection during treatment, often apply to many drugs in a particular class. Combining different drug classes into a single microbicide should provide greater protection against the growing variability observed in HIV. Our work has identified the beneficial effects of combining the fusion inhibitor RC-101 and the RT inhibitor CSIC to prevent transmission of clinically isolated and drug-resistant HIV-1.Several aspects of HIV-1 virulence and pathogenesis are mediated by the envelope protein gp41. Additionally, peptides derived from the gp41 ectodomain have been shown to induce chemotaxis in monocytes and neutrophils. While this chemotactic activity has been characterized, it is not known how these peptides could be produced under biological conditions. Our findings demonstrate that the epithelial serine protease matriptase efficiently cleaves the gp41 HR1 region at conserved residues into a chemotactic peptide.Here, we present evidence that advances our understanding of resistance to peptide entry inhibitors, reveals a potential benefit to combining specific drugs in an antiviral microbicide, and identifies a pathway by which HIV-1 may generate peptides to exploit host immunity. This work thereby facilitates improved methods in countering drug resistance and the development of new antiviral approaches to prevent HIV-1 transmission. Additionally, we have revealed basic mechanistic evidence that shed light on our current understanding of HIV-1 infection. Specifically, our focus on gp41 provides much needed insight into its role in membrane fusion, drug susceptibility, and modification of host responses.
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Date Issued: 2014
Identifier: CFE0005560, ucf:50286
Format: Document (PDF)
PURL: http://purl.flvc.org/ucf/fd/CFE0005560

Title: MIGRANT CHILD.
Creator: Sheperd, Nicholas, Thaxton, Terry, University of Central Florida
Abstract / Description: Migrant Child is a poetry collection about injustice in the United States of America and the international community. The purpose of the collection is to humanize social injustice in the present, so as to show the reader that discrimination still happens in the United States in 2016. To that end, the collection draws on comparisons from civil rights movements of the 1960s and from present day. It is also meant to reflect injustices the author experiences in his own life. The poetry collection...
Show moreMigrant Child is a poetry collection about injustice in the United States of America and the international community. The purpose of the collection is to humanize social injustice in the present, so as to show the reader that discrimination still happens in the United States in 2016. To that end, the collection draws on comparisons from civil rights movements of the 1960s and from present day. It is also meant to reflect injustices the author experiences in his own life. The poetry collection was created after the author spent six months volunteering throughout the State of Florida. The poems in the collection center around Hispanic communities in the United States, refugees seeking asylum, individuals living HIV and AIDS, male rape, and familial abuse. Several poems are written in the epigraph format, so as to place the reader in the author's desired mindset for that particular poem. In addition, multiple poems in this collection have been inspired by the poets Yusef Komunyakaa, Sylvia Plath, Anne Sexton, and Allen Ginsburg. In present day, discrimination and prejudice are still experienced by minority communities in the United States, and Migrant Child is not by any extent an exhaustive list of all communities that are, in the present, experiencing social injustice.
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Date Issued: 2016
Identifier: CFH2000107, ucf:45957
Format: Document (PDF)
PURL: http://purl.flvc.org/ucf/fd/CFH2000107

Title: THE ROLE OF BREASTFEEDING IN MOTHER-TO-CHILD TRANSMISSION OF HIV/AIDS: A COMPARATIVE CASE STUDY OF THREE COUNTRIES.
Creator: Cherukuri, Anjali, Borgon, Robert, University of Central Florida
Abstract / Description: The HIV pandemic has affected millions of people around the world both medically and socially, since there is a stigma associated with this disease. Common methods of transmission include sexual intercourse and sharing needles, but there are other lesser known methods through which people can contract this disease. One such way is mother-to-child transmission (MTCT), in which a mother could transmit the virus to her child either during pregnancy, childbirth, or through breastfeeding. This...
Show moreThe HIV pandemic has affected millions of people around the world both medically and socially, since there is a stigma associated with this disease. Common methods of transmission include sexual intercourse and sharing needles, but there are other lesser known methods through which people can contract this disease. One such way is mother-to-child transmission (MTCT), in which a mother could transmit the virus to her child either during pregnancy, childbirth, or through breastfeeding. This paper focuses on the role of breastfeeding in the transmission of HIV from mother to child. Many studies have investigated how breastfeeding results in the transmission of the virus, and effective common treatment methods have been established. However, the issue of MTCT of HIV still exists even though it can easily be eradicated with the proper techniques. This suggests that there are still factors that contribute to HIV transmission from mother to child that have yet to be eliminated. Thus, this paper reviews the breastfeeding rates and breastfeeding practices of three different countries: South Africa, India, and the United Kingdom. This paper analyzes epidemiological data, studies from medical journals, and studies from anthropology journals to determine what social influences surround breastfeeding practices in each of these countries to see how these may contribute to MTCT of HIV via breastfeeding. While there were no apparent trends between child HIV prevalence rates and breastfeeding rates in these countries, there were some social and cultural factors that were similar across all three nations. This information may be useful in creating more effective treatment plans that are conducive to the social environments in these countries.
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Date Issued: 2017
Identifier: CFH2000204, ucf:46034
Format: Document (PDF)
PURL: http://purl.flvc.org/ucf/fd/CFH2000204

Title: Characterization of Innate Immunity in the Female Reproductive Tract for the Prevention of HIV Acquisition.
Creator: Eade, Colleen, Cole, Alexander, Jewett, Travis, Naser, Saleh, Khaled, Annette, University of Central Florida
Abstract / Description: Human immunodeficiency virus (HIV) infects 30 million people worldwide. In sub-Saharan Africa, the region most affected by HIV, women comprise 60% of the infected population. Heterosexual transmission is a major mode of viral acquisition, mandating further research of the process and prevention of HIV acquisition via the female reproductive tract (FRT). The FRT is a dynamic environment, protected by host immune mechanisms and commensal microbes. The disruption of either of these elements can...
Show moreHuman immunodeficiency virus (HIV) infects 30 million people worldwide. In sub-Saharan Africa, the region most affected by HIV, women comprise 60% of the infected population. Heterosexual transmission is a major mode of viral acquisition, mandating further research of the process and prevention of HIV acquisition via the female reproductive tract (FRT). The FRT is a dynamic environment, protected by host immune mechanisms and commensal microbes. The disruption of either of these elements can increase susceptibility to HIV. Accordingly, one common risk factor for HIV acquisition is the microbial shift condition known as bacterial vaginosis (BV), which is characterized by the displacement of healthy lactobacilli by an overgrowth of pathogenic bacteria. As the bacteria responsible for BV pathogenicity and their interactions with host immunity are not understood, we sought to evaluate the effects of BV-associated bacteria on reproductive epithelia. Here we have characterized the interaction between BV-associated bacteria and the female reproductive tract by measuring cytokine and defensin induction in FRT epithelial cells following bacterial inoculation. Four BV-associated bacteria were evaluated alongside six lactobacilli for a comparative assessment. Our model showed good agreement with clinical BV trends; we observed a distinct cytokine and human ?-defensin-2 response to BV-associated bacteria, especially Atopobium vaginae, compared to most lactobacilli. One lactobacillus species, Lactobacillus vaginalis, induced an immune response similar to that elicited by BV-associated bacteria. These data provide an important prioritization of BV-associated bacteria and support further characterization of reproductive bacteria and their interactions with host epithelia. We next evaluated the effect of this interaction on HIV infection by investigating the soluble effectors secreted when FRT epithelial cells were cocultured with A. vaginae. We observed increased proviral activity mediated by secreted low molecular weight effectors, and determined that this activity was not likely mediated by cytokine responses. Instead, we identified a complex mixture containing several upregulated host proteins. Selected individual proteins from the mixture exhibited HIV-enhancing activity only when applied with the complex mixture of proviral factors, suggesting that HIV enhancement might be mediated by synergistic effects.In addition to characterizing the immune interactions that mediate the enhanced HIV acquisition associated with BV, we also evaluated the safety and efficacy of RC-101, a candidate vaginal microbicide being developed for the prevention of HIV transmission. RC-101 has been effective and well tolerated in preliminary cell culture and macaque models. However, the effect of RC-101 on primary vaginal tissues and resident vaginal microflora requires further evaluation. Here, we treated primary vaginal tissues and vaginal bacteria, both pathogenic and commensal, with RC-101 to investigate compatibility of this microbicide with FRT tissue and microflora. RC-101 was well tolerated by host tissues and commensal vaginal bacteria, while BV-associated bacteria were inhibited by RC-101. By establishing vaginal microflora, the specific antibacterial activity of RC-101 may provide a dual mechanism of HIV protection.
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Date Issued: 2013
Identifier: CFE0004677, ucf:49867
Format: Document (PDF)
PURL: http://purl.flvc.org/ucf/fd/CFE0004677

Title: EXPLORING THE RELATIONSHIP BETWEEN INTIMATE PARTNER VIOLENCE AND HIV RISK PROPENSITY IN AFRICAN AMERICAN WOMEN.
Creator: Josephs, Lauren, Abel, Eileen, University of Central Florida
Abstract / Description: ABSTRACT This study explored the relationship between Intimate Partner Violence and HIV Risk-Propensity in African-American women. Current literature on HIV reveals that one of the groups most seriously impacted by the continued scourge of HIV is African-American heterosexual women. An anonymous questionnaire was completed by a community based sample of 200 African American women with a varied history of intimate partner violence to (1) explore whether a relationship exists between Intimate...
Show moreABSTRACT This study explored the relationship between Intimate Partner Violence and HIV Risk-Propensity in African-American women. Current literature on HIV reveals that one of the groups most seriously impacted by the continued scourge of HIV is African-American heterosexual women. An anonymous questionnaire was completed by a community based sample of 200 African American women with a varied history of intimate partner violence to (1) explore whether a relationship exists between Intimate Partner Violence and sexual coercion in African-American women (2) determine whether a relationship exists between sexual coercion and HIV Risk in African-American women (3) determine if the frequency of intimate partner violence impacts the HIV-risk of African-American Women (4) determine if the ability of African-American women to negotiate condom use is affected by being in a violent relationship, and (5) determine if there is a relationship between Intimate Partner Violence and increased HIV-risk propensity among African-American women. Various statistical techniques, including structural equation modeling (SEM), bivariate correlation analyses and ANOVA were used to examine the data. A strong positive correlation was found to exist between sexual coercion and Intimate Partner Violence. Specifically, the frequency of physical abuse, and feeling frightened by what a partner says or does were found to be highly correlated with sexual coercion. Moderate levels of correlation were also found between sexual coercion and frequency of being emotionally abused. Statistically significant regression weights indicate that when Intimate Partner Violence increases, sexual coercion also increases. Moderate and high correlations were also found between sexual coercion and ability to negotiate condom use. Statistically significant standardized regression weights indicate that as sexual coercion increases HIV Risk also increases. Moderate correlations also exist between being in a violent relationship and being able to negotiate condom use. Standardized regression weights reveal that as IPV increases HIV Risk also increases. The frequency of Intimate Partner Violence was also shown to impact the levels of sexual coercion, likelihood of having a partner with a STD, and the likelihood of have a partner who uses intravenous drugs. In the effort to fortify prevention strategies, and reduce the rates of HIV infection in African-American women, additional factors that impact disease transmission were discussed and suggestions for future research were made.
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Date Issued: 2006
Identifier: CFE0001454, ucf:47053
Format: Document (PDF)
PURL: http://purl.flvc.org/ucf/fd/CFE0001454

Title: PROTECTION OF THE FEMALE REPRODUCTIVE TRACT IN THE PREVENTION OF HIV.
Creator: Diaz, Camila, Cole, Alexander, University of Central Florida
Abstract / Description: Worldwide, more than half of all HIV-infected individuals are women. Since mucosal surfaces are the primary gateway for HIV entry, maintaining the integrity of the female reproductive tract (FRT) is essential for preventing infection. The FRT employs many immune mechanisms that serve as the first line of defense against HIV transmission. Among these are vaginal fluid secretions rich in antimicrobial peptides, and commensal bacteria that colonize the vagina and prevent infections. We sought to...
Show moreWorldwide, more than half of all HIV-infected individuals are women. Since mucosal surfaces are the primary gateway for HIV entry, maintaining the integrity of the female reproductive tract (FRT) is essential for preventing infection. The FRT employs many immune mechanisms that serve as the first line of defense against HIV transmission. Among these are vaginal fluid secretions rich in antimicrobial peptides, and commensal bacteria that colonize the vagina and prevent infections. We sought to study vaginal fluid as an innate immune component of the FRT in the prevention of HIV infection. Additionally, we investigated the anti-HIV microbicide candidate RC-101 as a possible treatment against pathogenic bacteria that disrupt the healthy microbiota of the FRT and create a suboptimal immune state that increases host susceptibility to viruses, such as HIV. Here we report that vaginal fluid collected from healthy females inhibits HIV infection. Moreover, our studies reveal that vaginal fluid collected from Black and White women exhibit disparate anti-HIV activity, possibly rendering Black women more susceptible to HIV infection. In addition, we show that RC-101, which is active against HIV, can also inhibit pathogenic bacteria that compromise FRT innate immunity, providing a dual mechanism of protection against HIV acquisition. Overall, these findings show that vaginal fluid is an important part of female innate immunity that protects the host from heterosexual HIV acquisition. Furthermore, the microbicide RC-101 may prevent HIV infection by both directly preventing viral entry, and by restricting the growth of pathogenic bacteria that disrupt the protective commensal vaginal flora. Together, innate mechanisms and bolstered protection present a multifaceted approach to maintaining effective host immunity.
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Date Issued: 2012
Identifier: CFH0004150, ucf:44842
Format: Document (PDF)
PURL: http://purl.flvc.org/ucf/fd/CFH0004150

Title: AN EVALUATION OF HIV/AIDS INCIDENCE REDUCTION AND AWARENESS-RAISING INTERVENTIONS INSPIRED BY THE SONAGACHI PROJECT AND THE 100% CONDOM USE PROGRAM.
Creator: Fernandez, Nicole, Rovito, Michael, University of Central Florida
Abstract / Description: In the past thirty years, HIV/AIDS (Human Immunodeficiency Virus/Acquired Immune Deficiency Syndrome) transformed from a mysteriously lethal disease affecting limited portions of the population to a true global pandemic. Although HIV/AIDS is responsible for the deaths of approximately 30 million people worldwide, prevalence rates are now increasing significantly due to increasing survival rates. However, overall increasing incidence rates now serve as a primary concern for researchers. Avert ...
Show moreIn the past thirty years, HIV/AIDS (Human Immunodeficiency Virus/Acquired Immune Deficiency Syndrome) transformed from a mysteriously lethal disease affecting limited portions of the population to a true global pandemic. Although HIV/AIDS is responsible for the deaths of approximately 30 million people worldwide, prevalence rates are now increasing significantly due to increasing survival rates. However, overall increasing incidence rates now serve as a primary concern for researchers. Avert (2011) suggests that there is a lack of behavioral interventions and prevention programs aimed at decreasing the number of newly affected individuals. This is problematic as it may create not only physical and mental stress upon patients but also a source of financial and resource stress upon service organizations. In Asia, HIV/AIDS is primarily apparent in three high-risk groups: sex workers, men who have sex with men, and intravenous drug users (Avert, 2011). Service organizations target these high-risk groups with prevention programs in order to decrease infection rates and raise general awareness of the disease. This study aims to evaluate two HIV/AIDS prevention program theories(the Sonagachi Project and the 100% Condom Use Program) and the studies that implement them. This proposed evaluation assesses the effectiveness of these HIV/AIDS prevention programs in reducing infection rates and raising awareness of the disease. Due to the widespread use and apparent effectiveness of the 100% Condom Use Program and The Sonagachi Project, this study aims to assess the interventions in lessening HIV infection rates and critique the methods outlined by both. This thesis also employs a systemic review of the literature by using the program theories of The 100% Condom Use Program and The Sonagachi Project.
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Date Issued: 2012
Identifier: CFH0004180, ucf:44839
Format: Document (PDF)
PURL: http://purl.flvc.org/ucf/fd/CFH0004180

Title: SEX EDUCATION POLICY IN FLORIDA: STRATEGIES FOR CHANGE.
Creator: Cawley, Jenna, Fine, Terri Susan, University of Central Florida
Abstract / Description: Sex education policies and programs in Florida are largely dominated by the abstinence-only approach. This paper makes the case that abstinence-only education is a failing policy in Florida, and evaluates strategies advocates may use in order to accomplish reform. Three different strategies are evaluated: countywide school district reform, statewide rejection of federal abstinence-only funding, and statewide standardization of sex education via legislation. Contrasts are drawn between all...
Show moreSex education policies and programs in Florida are largely dominated by the abstinence-only approach. This paper makes the case that abstinence-only education is a failing policy in Florida, and evaluates strategies advocates may use in order to accomplish reform. Three different strategies are evaluated: countywide school district reform, statewide rejection of federal abstinence-only funding, and statewide standardization of sex education via legislation. Contrasts are drawn between all three strategies with regard to their potential impact on sex education policy in Florida, viability, and the challenges they present to advocates. This paper concludes that statewide standardization of sex education in Florida represents the best way to remedy the problem of insufficient sex education, but is unlikely to occur without increased bipartisan support in the Florida legislature. Statewide rejection of Title V federal abstinence-only funds remains an important policy goal for the purpose of accomplishing an end to federal abstinence grants but would likely achieve very little for Florida's students. Countywide sex education changes are thus far the only substantive victory for sex education advocates in Florida and should be instituted across the state with advocates taking special care to engage teachers, medical professionals, parents and local community leaders.
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Date Issued: 2008
Identifier: CFE0002259, ucf:47829
Format: Document (PDF)
PURL: http://purl.flvc.org/ucf/fd/CFE0002259

HIV (13)	+ -
HIV/AIDS (7)	+ -
retrocyclin (3)	+ -
Botswana (2)	+ -
HIV stigma (2)	+ -

Kenya (2)	+ -
antimicrobial (2)	+ -
bacterial vaginosis (2)	+ -
defensin (2)	+ -
hiv (2)	+ -
innate immunity (2)	+ -
stigma (2)	+ -
AIDS (1)	+ -
Abuse (1)	+ -
African American Women (1)	+ -
Asia (1)	+ -
Atopobium (1)	+ -
BMSM (1)	+ -
BMSM+ (1)	+ -
BV (1)	+ -
Bacterial Vaginosis (1)	+ -
Breastfeeding (1)	+ -
Caldwell (1)	+ -
Caregivers in Kenya (1)	+ -
Counseling (1)	+ -
Covalent Inhibition (1)	+ -
Dillon Caldwell (1)	+ -
Domestic Violence (1)	+ -
Emotion Work (1)	+ -
Emotional Labor (1)	+ -

ETD Collection (17)	+ -
Honors Collection (10)	+ -

University of Central Florida (27)	+ -
Cole, Alexander (7)	+ -
Gay, David (2)	+ -
Abel, Eileen (1)	+ -
Anthony, Amanda (1)	+ -

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Beidel, Deborah (1)	+ -
Blackwell, Christopher (1)	+ -
Boote, David (1)	+ -
Borgon, Robert (1)	+ -
Caldwell, James (1)	+ -
Cawley, Jenna (1)	+ -
Chai, Karl (1)	+ -
Chen, Bo (1)	+ -
Cherukuri, Anjali (1)	+ -
Comerford, Amy (1)	+ -
Daire, Andrew (1)	+ -
Diaz, Camila (1)	+ -
Dollarhide, Collette (1)	+ -
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Eade, Colleen (1)	+ -
Fernandez, Nicole (1)	+ -
Fine, Terri Susan (1)	+ -
Fuhrman, Christopher (1)	+ -
Hagedorn, William (1)	+ -
Heard, Nevin (1)	+ -
Hernandez, Florencio (1)	+ -
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