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- Title
- COMPARATIVE STUDY OF ETHANOL AND METHANOL ELECTRO-OXIDATION ON A PLATINUM/CERIA COMPOSITE ELECTRODE IN ALKALINE AND ACID SOLUTIONS: ELECTRO-CATALYTIC PERFORMANCE AND REACTION KINETICS.
- Creator
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Hidalgo, Carlos, Diaz, Diego, University of Central Florida
- Abstract / Description
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A comparative study of the electro-oxidation of ethanol and methanol was carried out on a Pt/ceria composite electrode prepared by electro-deposition. Modification of the Pt electrode was realized by co-deposition from a 1.0 mM K2PtCl6 solution that also contained a 20 mM suspension of ceria. The electro-catalytic activities and stabilities of the Pt/ceria catalyst towards ethanol electro-oxidation reactions (EOR) and methanol electro-oxidation reactions (MOR) were investigated by...
Show moreA comparative study of the electro-oxidation of ethanol and methanol was carried out on a Pt/ceria composite electrode prepared by electro-deposition. Modification of the Pt electrode was realized by co-deposition from a 1.0 mM K2PtCl6 solution that also contained a 20 mM suspension of ceria. The electro-catalytic activities and stabilities of the Pt/ceria catalyst towards ethanol electro-oxidation reactions (EOR) and methanol electro-oxidation reactions (MOR) were investigated by potentiodynamic and potentiostatic methods in 0.5 M sulfuric acid and 1.0 M sodium hydroxide solutions at various concentrations of ethanol and methanol. The kinetics of ethanol and methanol on a Pt/ceria composite electrode were measured in 0.5 M sulfuric acid and 1.0 M sodium hydroxide solutions using a rotating disk electrode (RDE). Cyclic voltammetry was employed in temperatures ranging from 15 to 55°C to provide quantitative and qualitative information on the kinetics of alcohol oxidation. The temperature dependence of the electro-catalytic activities afforded the determination of apparent activation energies for ethanol and methanol oxidation.
Show less - Date Issued
- 2011
- Identifier
- CFE0003628, ucf:48853
- Format
- Document (PDF)
- PURL
- http://purl.flvc.org/ucf/fd/CFE0003628
- Title
- DEVELOPMENT OF AN ALKALINE PHOSPHATASE REPORTER SYSTEM FOR USE IN THE LYME DISEASE SPIROCHETE BORRELIA BURGDORFERI.
- Creator
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Sutchu, Selina, Jewett, Mollie, University of Central Florida
- Abstract / Description
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The use of the periplasmic alkaline phosphatase (PhoA) reporter protein from E. coli has been critical for definition of the topology of transmembrane proteins of multiple bacterial species. This report demonstrates development of a PhoA reporter system in B. burgdorferi. Codon usage of the E. coli phoA in B. burgdorferi was analyzed and an optimized version of the gene was obtained. In order to assess the differential activity of the reporter system, two optimized PhoA-fusion construct using...
Show moreThe use of the periplasmic alkaline phosphatase (PhoA) reporter protein from E. coli has been critical for definition of the topology of transmembrane proteins of multiple bacterial species. This report demonstrates development of a PhoA reporter system in B. burgdorferi. Codon usage of the E. coli phoA in B. burgdorferi was analyzed and an optimized version of the gene was obtained. In order to assess the differential activity of the reporter system, two optimized PhoA-fusion construct using B. burgdorferi proteins were engineered: one using the periplasmic protein OppAIV and one using the cytoplasmic protein PncA. The activity of PhoA requires periplasmic localization. The periplasmic OppAIV-PhoA fusion as well as the cytoplasmic PncA-PhoA fusion produced detectable PhoA protein in E. coli and in B. burgdorferi. The periplasmic fusion construct, but not the cytoplasmic fusion construct, resulted in functional alkaline phosphatase (AP) activity in E. coli, as observed by blue colonies on agar plates containing a chromogenic substrate for AP. In contrast, both of the fusion constructs produced limited detectable levels of functional alkaline phosphatase activity in B. burgdorferi, as observed by yellow color change in liquid protein lysate containing a chromogenic substrate for AP. Development of a PhoA fusion reporter system for use in B. burgdorferi will provide a new molecular genetics tool for analyzing the topology of B. burgdorferi transmembrane proteins. These types of studies are critical for understanding the function of B. burgdorferi transport systems and may identify novel molecular approaches for the treatment of Lyme disease.
Show less - Date Issued
- 2013
- Identifier
- CFH0004343, ucf:44985
- Format
- Document (PDF)
- PURL
- http://purl.flvc.org/ucf/fd/CFH0004343
