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- Title
- CONFOCAL LASER SCANNING MICROSCOPY AS A TOOL FOR THE INVESTIGATION OF TETRACYCLINE FLUORESCENCE IN ARCHAEOLOGICALHUMAN BONE.
- Creator
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Maggiano, Corey, Waterman, Jane, University of Central Florida
- Abstract / Description
-
Fluorochromes such as tetracycline have been used to label bone for histomorphometric analysis, measuring bone formation, growth, maintenance, and pathology. More recently, similar fluorescence has been observed in ancient human bone. Attributed to tetracycline (TC) exposure, this phenomenon could affect various aspects of health during life and/or preservation of remains postmortem. Standard epifluorescence microscopy is the most common tool employed in the analysis of these labels. Though...
Show moreFluorochromes such as tetracycline have been used to label bone for histomorphometric analysis, measuring bone formation, growth, maintenance, and pathology. More recently, similar fluorescence has been observed in ancient human bone. Attributed to tetracycline (TC) exposure, this phenomenon could affect various aspects of health during life and/or preservation of remains postmortem. Standard epifluorescence microscopy is the most common tool employed in the analysis of these labels. Though valuable, this technique is limited by its inability to penetrate bone three-dimensionally and its inclusion of out-of-focus light, possibly disrupting accurate analysis. Confocal Laser Scanning Microscopy (CLSM) has been demonstrated as a valuable tool for three-dimensional histology. Its application to the study of compact bone fluorescence has been lacking, especially in archaeological and forensic sciences. In the following two papers, modern TC-controlled bone is compared to well preserved archaeological bone recovered from the Dakhleh Oasis, Egypt, using both standard wide-field and more modern confocal techniques for imaging and analysis. Spectral analysis via CLSM shows that both modern and ancient fluorescent labels in bone share the exact same fluorescence emission peak at 525 nm. Differences in the shape of the spectral curve and photobleaching characteristics are discussed. In addition, CLSM's high-resolution two- and three-dimensional imaging capabilities (in polarized light, scattered light, and fluorescence light) are found to increase the flexibility and creativity of investigations into the occurrence of tetracycline labels in archaeological bone and could have added benefits for modern medical and anatomical experimentation.
Show less - Date Issued
- 2005
- Identifier
- CFE0000836, ucf:46680
- Format
- Document (PDF)
- PURL
- http://purl.flvc.org/ucf/fd/CFE0000836
- Title
- Differential Diagnoses of Temporal Bone Defects and Zygomatic Bone Lesions Found in Fetal and Infant Individuals from the Kellis 2 Cemetery, Dakhleh Oasis, Egypt.
- Creator
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Jardine, Brittany, Dupras, Tosha, Wheeler, Sandra, Schultz, John, University of Central Florida
- Abstract / Description
-
The Kellis 2 cemetery site within the Dakhleh Oasis, Egypt provides a unique study opportunity due to the large number of infant, perinatal, and fetal individuals that have been recovered. Several of the infant and fetal remains have undiagnosed circular defects on the temporal bone, and others have undiagnosed lesions on the zygomatic bone. Of the 268 individuals under one year of age that have been analyzed from the Kellis 2 cemetery, twenty-six individuals have the temporal bone defect and...
Show moreThe Kellis 2 cemetery site within the Dakhleh Oasis, Egypt provides a unique study opportunity due to the large number of infant, perinatal, and fetal individuals that have been recovered. Several of the infant and fetal remains have undiagnosed circular defects on the temporal bone, and others have undiagnosed lesions on the zygomatic bone. Of the 268 individuals under one year of age that have been analyzed from the Kellis 2 cemetery, twenty-six individuals have the temporal bone defect and six have the zygomatic bone lesions. A survey of clinical and paleopathological research provided possible pathological conditions that could cause abnormalities such as defects or lesions on the temporal bones or zygomatic bones in the fetal and infant population. For this study, the temporal bone defects and zygomatic bone lesions were macroscopically observed and a descriptive analysis was created. The information garnered from the literature survey was then compared to the individuals from the Kellis 2 cemetery that had the temporal bone defects and zygomatic bone lesions to create a differential diagnosis. A differential diagnosis of the temporal bone defects includes mastoid emissary vein defects and petrosquamous sinus anomalies. A differential diagnosis of the zygomatic bone lesions includes scurvy. Contributing factors may also have been present in order for these defects and lesions to occur. Creating a differential diagnosis of the defects and lesions can provide information on the health, growth, and morbidity of the youngest members of the society related to the Kellis 2 cemetery.
Show less - Date Issued
- 2011
- Identifier
- CFE0004122, ucf:49104
- Format
- Document (PDF)
- PURL
- http://purl.flvc.org/ucf/fd/CFE0004122
- Title
- SYNTHESIS AND CHARACTERIZATION OF NANOCRYSTALLINE HYDROXYAPATITE POWDER; AND THE EFFECTS OF OXIDE-BASED SINTERING ADDITIVES ON TRICALCIUM PHOSPHATE.
- Creator
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Bhatt, Himesh, Kalita, Samar, University of Central Florida
- Abstract / Description
-
Calcium phosphate (CP) materials have been used extensively for bone replacement and augmentation due to their similarity to the mineral component of bone. In addition to being non-toxic, they are biocompatible, not recognized as foreign materials in vivo, and most importantly, exhibit osteoconductive behavior, being able to help in bone formation during healing. CPs form an intimate physicochemical bond with the host tissue, termed osseointegration. However the main limitation of calcium...
Show moreCalcium phosphate (CP) materials have been used extensively for bone replacement and augmentation due to their similarity to the mineral component of bone. In addition to being non-toxic, they are biocompatible, not recognized as foreign materials in vivo, and most importantly, exhibit osteoconductive behavior, being able to help in bone formation during healing. CPs form an intimate physicochemical bond with the host tissue, termed osseointegration. However the main limitation of calcium phosphates is their inherent brittle nature and poor mechanical performance under complex stress states. As a result, these materials have been used clinically only in non-load-bearing applications, primarily as granules. The primary goal of this research was to enhance mechanical performance of CPs, tricalcium phosphate (TCP) and hydroxyapatite (HAp) to be precise, in an attempt to develop controlled strength-loss resorbable/ bioactive ceramic bone-grafts for hard tissue engineering. In my work on TCP, I selected and introduced small quantities of single and multi-oxide sintering additives in TCP, to study their influence on sintering behavior, densification, mechanical properties and biodegradation/ biomechanical properties in vitro. Through this research, I could improve mechanical performance of ƒÒ-tricalcium phosphate (ƒÒ-TCP) and controlled its rate of biodegradation by introducing of certain additives. In my second work, I improved mechanical performance of HAp (Ca10(PO4)6(OH)2 by reducing particle-size of the powder through the synthesis of stoichiometric, nanocrystalline, single phase HAp powder in the range of 2-20 nm. Synthesis of powder was accomplished via a modified low temperature sol-gel technique using ethanol/ water as solvent.
Show less - Date Issued
- 2005
- Identifier
- CFE0000594, ucf:46482
- Format
- Document (PDF)
- PURL
- http://purl.flvc.org/ucf/fd/CFE0000594
- Title
- STRESS SHIELDING MINIMIZED IN FEMORAL HIP IMPLANTS: A FINITE ELEMENT MODEL OPTIMIZED BY VIRTUAL COMPATIBILITY.
- Creator
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feldt, christian, Chew, Larry, University of Central Florida
- Abstract / Description
-
Bone mechanics and traditional implant materials produce a recurring problem for patients of total hip arthroplasty (THA): the bone is "shielded" from the loading it has become accustomed to over many years of development. Bone adheres to what is called "Wolff's Law", meaning it is an adaptive structure which adjusts its geometry based on the loads experienced over its life. As the new femoral hip implant transmits reduced stresses to the remaining bone, bone tissue atrophies at the interface...
Show moreBone mechanics and traditional implant materials produce a recurring problem for patients of total hip arthroplasty (THA): the bone is "shielded" from the loading it has become accustomed to over many years of development. Bone adheres to what is called "Wolff's Law", meaning it is an adaptive structure which adjusts its geometry based on the loads experienced over its life. As the new femoral hip implant transmits reduced stresses to the remaining bone, bone tissue atrophies at the interface, permitting loosening of the implant, pain, and thereby obliging additional surgery to correct the issue. In the present work, a methodology is endeavored for creating an innovative design for femoral hip implants. The approach uncouples the finite element implant model from the bone model, in order to focus solely on expected behavior within the implant while considering the varying material behavior in unique directions and locations. The implant's internal geometry is optimized in order to better match typical, intact bone conditions. The eventual design reduces extreme changes in stresses within remnant bone such that the implant will remain implanted for greater periods of time without additional surgical attention.
Show less - Date Issued
- 2011
- Identifier
- CFE0004051, ucf:52891
- Format
- Document (PDF)
- PURL
- http://purl.flvc.org/ucf/fd/CFE0004051
- Title
- EXAMINATION OF THE REHABILITATION PROTOCOL OF TRAUMATIC TRANSFEMORAL AMPUTEES AND HOW TO PREVENT BONE MINERAL DENSITY LOSS.
- Creator
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Jenkinson, Emily R, Fisher, Thomas, University of Central Florida
- Abstract / Description
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The purpose of this literature review was to identify any adaptations that could be made to the rehabilitation process for Traumatic Transfemoral Amputees. Traumatic Transfemoral Amputation is particularly debilitating with the amputees encountering many obstacles throughout the rehabilitation process. These obstacles can prevent the return to pre-morbid functioning. With an ever-increasing number of amputees within the United States, it is imperative the rehabilitation process be addressed....
Show moreThe purpose of this literature review was to identify any adaptations that could be made to the rehabilitation process for Traumatic Transfemoral Amputees. Traumatic Transfemoral Amputation is particularly debilitating with the amputees encountering many obstacles throughout the rehabilitation process. These obstacles can prevent the return to pre-morbid functioning. With an ever-increasing number of amputees within the United States, it is imperative the rehabilitation process be addressed. This literature review addresses possible adjustments in the initial stages of rehabilitation examining the post-operative, pre-prosthetic, and prosthetic rehabilitation stage to enhance the physical functioning for the amputee. This comprehensive literature review encompassing 63 academic and medical journals analyzes the research literature regarding each of the three stages of the post-operative procedure. The literature review synthesizes the research findings to see how procedures may be adapted to reduce the risk of further co-morbidities such as loss of bone mineral density and disuse atrophy. Loss of bone mineral density and disuse atrophy are the major contributing factors to the amputees decreased mobility. Reducing this loss can be addressed within the initial post-operative, pre-prosthetic, and prosthetic rehabilitation stages. Further research is required to examine the efficacy of these alterations in relation to this specific population.
Show less - Date Issued
- 2017
- Identifier
- CFH2000268, ucf:45964
- Format
- Document (PDF)
- PURL
- http://purl.flvc.org/ucf/fd/CFH2000268
- Title
- DEVELOPMENT OF THERMALLY PROCESSED NANOCOMPOSITES WITH CONTROLLED SURFACES.
- Creator
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Georgieva, Petya, Seal, Sudipta, University of Central Florida
- Abstract / Description
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The ever increasing need for technology development requires the integration of inexpensive, light weight and high strength materials which are able to meet the high standards and specifications for various engineering applications. The intention of this work is to show that the suitable material selection and the utilization of plasma spray processing can be of potential interest to a large number of industrial, biomedical and everyday life applications. This research demonstrates also that...
Show moreThe ever increasing need for technology development requires the integration of inexpensive, light weight and high strength materials which are able to meet the high standards and specifications for various engineering applications. The intention of this work is to show that the suitable material selection and the utilization of plasma spray processing can be of potential interest to a large number of industrial, biomedical and everyday life applications. This research demonstrates also that plasma processing is a promising engineering tool for multifunctional coatings and near-net-shape manufacturing. Further, the theoretical and experimental results are combined in order to explain the mechanisms behind nanostructure retention and enhanced properties. Proper design of experiments, an appropriate material selection and experimental methodology are discussed herein. The experimental conditions were optimized in order to achieve the best materials properties according to their explicit properties and functions. Specific materials were consolidated according to their prospective performance and applications: 1) Plasma spraying of nano-Ceria-stabilized Zirconia free form part for stem cells scaffolds, 2) Plasma spraying of FeCrAlY on Ti-alloy plate, additionally coated with nano-size Hydroxyapatite for bone tissue engineering, 3) Wire-arc spraying of nano-based steel wires for aerospace and automotive applications. The performance and characteristics of all of the developed coatings and free-form-parts are evaluated using state-of-the art characterization techniques.
Show less - Date Issued
- 2006
- Identifier
- CFE0001153, ucf:46871
- Format
- Document (PDF)
- PURL
- http://purl.flvc.org/ucf/fd/CFE0001153
- Title
- CHARACTERISTICS OF THE ADULT FEMALE ENDURANCE RUNNER: A SURVEY.
- Creator
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Gabriel, Stephanie F, Rothschild, Carey, University of Central Florida
- Abstract / Description
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Objective: This study investigated the behaviors and characteristics of the adult female endurance runner and potential components of the female athlete triad (FAT). The FAT consists of three components that are interrelated: low energy availability, menstrual dysfunction, and low bone mineral density. Low energy availability may occur with or without disordered eating. Reproduction becomes non-essential leading to irregular menstrual cycles. A reduction in estrogen levels may contribute to...
Show moreObjective: This study investigated the behaviors and characteristics of the adult female endurance runner and potential components of the female athlete triad (FAT). The FAT consists of three components that are interrelated: low energy availability, menstrual dysfunction, and low bone mineral density. Low energy availability may occur with or without disordered eating. Reproduction becomes non-essential leading to irregular menstrual cycles. A reduction in estrogen levels may contribute to low bone mineral density which may lead to stress fractures. Research investigating the FAT has primarily focused on adolescent and young adult females. Adult females training for endurance events may also be at risk for the FAT. Method: A survey was constructed and distributed to females in a local half-marathon and marathon training group in Central Florida. The data was collected at one point in time and no additional follow-up was required. The survey aimed to identify specific behaviors and characteristics related to components of the FAT and determine the potential prevalence in a small sample of female endurance athletes aged 18 and older. Results: 72 females with a mean age of 40.92(± 9.61) years completed the survey. Subjects had an average height of 163.60(±6.41) cm, weighed an average of 62.24(±10.05) kg and had 10 years of running experience. Conclusion: Adult female endurance runners demonstrate behaviors and characteristics that may be indicative of the FAT. Participants demonstrated signs of inadvertent or intentional low energy availability. These characteristics can be due to either body dissatisfaction or wanting to increase performance.
Show less - Date Issued
- 2017
- Identifier
- CFH2000173, ucf:45926
- Format
- Document (PDF)
- PURL
- http://purl.flvc.org/ucf/fd/CFH2000173
- Title
- Influence of Chitosan-Alginate Scaffold Stiffness on Bone Marrow Stromal Cell Differentiation.
- Creator
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Arias Ponce, Isabel, Florczyk, Stephen, Fang, Jiyu, Khaled, Annette, Leon, Lorraine, University of Central Florida
- Abstract / Description
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Tissue grafts are the gold standard for replacing large volume tissue defects. Yet, they present several risks, including infection, low functional outcomes, and reduced graft integrity. Tissue engineering (TE) combines cells and biomaterial scaffolds to foster tissue growth and remodeling. Bone marrow stromal cells (BMSCs) have been shown to respond to the stiffness of their microenvironment, resulting in differentiation into different lineages. 3D porous chitosan-alginate (CA) scaffolds...
Show moreTissue grafts are the gold standard for replacing large volume tissue defects. Yet, they present several risks, including infection, low functional outcomes, and reduced graft integrity. Tissue engineering (TE) combines cells and biomaterial scaffolds to foster tissue growth and remodeling. Bone marrow stromal cells (BMSCs) have been shown to respond to the stiffness of their microenvironment, resulting in differentiation into different lineages. 3D porous chitosan-alginate (CA) scaffolds have been previously demonstrated for bone TE with osteoblasts and BMSCs; however, only a single scaffold composition (4 wt%) was studied. Three CA scaffold compositions (2, 4, 6 wt% CA) were produced. Scanning electron microscopy images were obtained to determine average pore sizes for 2, 4, and 6 wt% CA scaffolds, which were 233, 208, and 146 ?m. Compression testing was performed on CA scaffolds in dry and wet conditions, where higher concentrations yielded higher stiffnesses ranging from 0.22 to 5.34 kPa and 21.1 to 47.3 Pa, respectively. Fourier transform infrared spectroscopy performed on the CA scaffolds confirmed polyelectrolyte complex formation for all compositions. Human BMSCs from three donors were seeded on CA scaffolds, cultured in growth media for 14 days, then cultured in adipogenic or osteogenic differentiation media for 28 days to promote differentiation. Our hypothesis was that scaffold stiffness would influence BMSC differentiation, with softer scaffolds promoting adipogenesis and stiffer scaffolds promoting osteogenesis. BMSCs formed multicellular spheroids in all CA scaffold concentrations, while the 2 wt% CA scaffolds had smaller spheroids compared to the 4 wt% and 6 wt% CA scaffolds. Osteogenic and adipogenic differentiation were evaluated with Alizarin Red and Oil Red O staining, respectively. While positive staining was observed in all scaffold compositions, more robust differentiation was expected, thereby disproving our hypothesis. The polysaccharide composition of the CA scaffolds likely contributed to the spheroid formation and limited differentiation.
Show less - Date Issued
- 2018
- Identifier
- CFE0007389, ucf:52061
- Format
- Document (PDF)
- PURL
- http://purl.flvc.org/ucf/fd/CFE0007389
- Title
- DIGITIZATION PROTOCOLS AND APPLICATIONS FOR LASER SCANNING HUMAN BONE IN FORENSIC ANTHROPOLOGY.
- Creator
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Filiault, Matthew, Schultz, John, University of Central Florida
- Abstract / Description
-
In medico-legal investigations involving unidentified skeletal remains, forensic anthropologists commonly assist law enforcement and medical examiners in their analysis and identification. The traditional documentation techniques employed by the forensic anthropologist during their analysis include notes, photographs, measurements and radiographic images. However, relevant visual information of the skeleton can be lacking in morphological details in 2D images. By creating a 3D representation...
Show moreIn medico-legal investigations involving unidentified skeletal remains, forensic anthropologists commonly assist law enforcement and medical examiners in their analysis and identification. The traditional documentation techniques employed by the forensic anthropologist during their analysis include notes, photographs, measurements and radiographic images. However, relevant visual information of the skeleton can be lacking in morphological details in 2D images. By creating a 3D representation of individual bones using a laser-scanner, it would be possible to overcome this limitation. Now that laser scanners have become increasingly affordable, this technology should be incorporated in the documentation methodologies of forensic anthropology laboratories. Unfortunately, this equipment is rarely used in forensic anthropology casework. The goal of this project is to investigate the possible visualization applications that can be created from digitized surface models of bone for use in medico-legal investigations. This research will be achieved in two phases. First, examples of human bone as well as replicas of bone will be scanned using a NextEngine™ laser scanner. In conjunction with this will be the exploration and documentation of protocols for scanning different bone types and processing the scan data for creating a 3D model. The second phase will investigate how the resulting 3D model can be used in lieu of the actual remains to achieve improved documentation methodologies through the use of several commercial computer graphics programs. The results demonstrate that an array of visual applications can be easily created from a 3D file of bone, including virtual curation, measurement, illustration and the virtual reconstruction of fragmented bone. Based on the findings of this project, the implementation of laser scanning technology is recommended for forensic anthropology labs to enhance documentation, analysis and presentation of human bone.
Show less - Date Issued
- 2012
- Identifier
- CFH0004287, ucf:44907
- Format
- Document (PDF)
- PURL
- http://purl.flvc.org/ucf/fd/CFH0004287
- Title
- REPRESENTATIONS OF GOTHIC CHILDREN IN CONTEMPORARY IRISH LITERATURE: A SEARCH FOR IDENTITY IN PATRICK MCCABE'S THE BUTCHER BOY, SEAMUS DEANE'S READING IN THE DARK, AND ANNA BURNS' NO BONES.
- Creator
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Ratte, Kelly, Campbell, James, University of Central Florida
- Abstract / Description
-
Ireland is not a country unfamiliar with trauma. It is an island widely known for its history with Vikings, famine, and as a colony of the English empire. Inevitably, then, these traumas surface in the literature from the nation. Much of the literature that was produced, especially after the decline in the Irish language after the Great Famine of the 1840s, focused on national identity. In the nineteenth century, there was a growing movement for Irish cultural identity, illustrated by authors...
Show moreIreland is not a country unfamiliar with trauma. It is an island widely known for its history with Vikings, famine, and as a colony of the English empire. Inevitably, then, these traumas surface in the literature from the nation. Much of the literature that was produced, especially after the decline in the Irish language after the Great Famine of the 1840s, focused on national identity. In the nineteenth century, there was a growing movement for Irish cultural identity, illustrated by authors John Millington Synge and William Butler Yeats; this movement was identified as the Gaelic Revival. Another movement in literature began in the nineteenth century and it reflected the social and political anxieties of the Anglo-Irish middle class in Ireland. This movement is the beginning of the Gothic genre in Irish literature. Dominated by authors such as Sheridan Le Fanu and Bram Stoker, Gothic novels used aspects of the sublime and the uncanny to express the fears and apprehensions that existed in Anglo-Irish identity in the nineteenth century. My goal in writing this thesis is to examine Gothic aspects of contemporary Irish fiction in order to address the anxieties of Irish identity after the Irish War of Independence that began in 1919 and the resulting division of Ireland into two countries. I will be examining Patrick McCabe's The Butcher Boy, Seamus Deane's Reading in the Dark, and Anna Burns' No Bones in order to evaluate their use of children amidst the trouble surrounding the formation of identity, both personal and national, in Northern Ireland. All three novels use gothic elements in order to produce an atmosphere of the uncanny (Freud); this effect is used to enlighten the theme of arrested development in national identity through the children protagonists, who are inescapably haunted by Ireland's repressed traumatic history. Specifically, I will be focusing on the use of ghosts, violence, and hauntings to illuminate the social anxieties felt by Northern Ireland after the Irish War of Independence.
Show less - Date Issued
- 2013
- Identifier
- CFH0004339, ucf:45002
- Format
- Document (PDF)
- PURL
- http://purl.flvc.org/ucf/fd/CFH0004339
