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The effects of enzymes on activated sludge floc
Title
The effects of enzymes on activated sludge floc.
Creator
Stoddart, Terry Lee, null, null, Natural Sciences
Abstract / Description
Florida Technological University College of Natural Sciences Thesis; Dialysed activated sludge was used as a substrate to test for enzymes which can hydrolyse activated sludge floc. Two hundred and fifty aerobic and anaerobic microorganisms were tested against activated sludge for the presence of hydrolytic enzymes. These included known genera and organisms obtained by various enrichment procedures. Anaerobic digester mixed liquor was contacted with activated sludge agar under anaerobic...
Show moreFlorida Technological University College of Natural Sciences Thesis; Dialysed activated sludge was used as a substrate to test for enzymes which can hydrolyse activated sludge floc. Two hundred and fifty aerobic and anaerobic microorganisms were tested against activated sludge for the presence of hydrolytic enzymes. These included known genera and organisms obtained by various enrichment procedures. Anaerobic digester mixed liquor was contacted with activated sludge agar under anaerobic conditions. None of the microorganisms present in the digester liquor hydrolysed the floc. The following commercial enzymes were contacted with activated sludge singly, in combination, and sequentially under various physical and chemical conditions: protease, lipase, cellulase, pectinase, phospholipase C, trypsin, and glucuronidase. Although commercial enzymes and various microorganisms reacted with known substrate controls neither the enzymes nor microorganisms employed affected the activated sludge floc. Treatment of activated sludge floc with ethylenediaminetetraacetic acid resulted in gross deflocculation and release of humic substances. The floc particle, probably a combination of polymers bound in a complex manner, is resistant to enzymatic degradation. Several workers have reported on the enzymatic hydrolysis of polymers produced by axenic cultures isolated from activated sludge floc. It is unlikely that the results of their work can be extrapolated to the complex structure of activated sludge floc particles.
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Date Issued
1977
Identifier
CFR0003513, ucf:53004
Format
Document (PDF)
PURL
http://purl.flvc.org/ucf/fd/CFR0003513
FIELD IMPLEMENTATION OF POLYACRYLAMIDE FOR RUNOFF FROM CONSTRUCTION SITES
Title
FIELD IMPLEMENTATION OF POLYACRYLAMIDE FOR RUNOFF FROM CONSTRUCTION SITES.
Creator
Chowdhury , Rafiqul, Chopra , Manoj, University of Central Florida
Abstract / Description
Polyacrylamide (PAM) is often used a part of a treatment train for the treatment of stormwater to reduce its turbidity. This study investigated the application of PAM within various treatment systems for a construction site environment. The general concept is to introduce hydraulic principles when placing PAM blocks within an open channel in order to yield high mixing energies leading to high turbidity removal efficiency. The first part of the study observed energy variation using a hydraulic...
Show morePolyacrylamide (PAM) is often used a part of a treatment train for the treatment of stormwater to reduce its turbidity. This study investigated the application of PAM within various treatment systems for a construction site environment. The general concept is to introduce hydraulic principles when placing PAM blocks within an open channel in order to yield high mixing energies leading to high turbidity removal efficiency. The first part of the study observed energy variation using a hydraulic flume for three dissimilar configurations. The flume was ultimately used to determine which configuration would be most beneficial when transposed into field-scale conditions. Three different configurations were tested in the flume, namely, the Jump configuration, Dispersion configuration and the Staggered configuration. The field-scale testing served as both justification of the findings within the controlled hydraulic flume and comprehension of the elements introduced within the field when attempting to reduce the turbidity of stormwater. As a result, the Dispersion configuration proved to be the most effective when removing turbidity and displayed a greater energy used for mixing within the open channel. Consequently, an analysis aid is developed based on calculations from the results of this study to better serve the sediment control industry when implementing PAM blocks within a treatment system. Recommendations are made for modification and future applications of the research conducted. This innovative approach has great potential for expansion and future applications. Continued research on this topic can expand on key elements such as solubility of the PAM, toxicity of the configuration within the field, and additional configurations that may yield more advantageous energy throughout the open channel.
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Date Issued
2011
Identifier
CFE0004017, ucf:49158
Format
Document (PDF)
PURL
http://purl.flvc.org/ucf/fd/CFE0004017
Mathematical Modeling of Carbon Removal in the A-Stage Activated Sludge System
Title
Mathematical Modeling of Carbon Removal in the A-Stage Activated Sludge System.
Creator
Nogaj, Thomas, Randall, Andrew, Duranceau, Steven, Chopra, Manoj, Jimenez, Jose, University of Central Florida
Abstract / Description
This research developed a dynamic activated sludge model (ASM) to better describe the overall removal of organic substrate, quantified as chemical oxygen demand (COD), from A-stage high rate activated sludge (HRAS) systems. This dynamic computer model is based on a modified ASM1 (Henze et al., 2000) model. It was determined early in the project that influent soluble COD, which is normally represented by a single state variable in ASM1, had to be subdivided into two state variables (SBs and...
Show moreThis research developed a dynamic activated sludge model (ASM) to better describe the overall removal of organic substrate, quantified as chemical oxygen demand (COD), from A-stage high rate activated sludge (HRAS) systems. This dynamic computer model is based on a modified ASM1 (Henze et al., 2000) model. It was determined early in the project that influent soluble COD, which is normally represented by a single state variable in ASM1, had to be subdivided into two state variables (SBs and SBf, or slow and fast fractions) to simulate the performance of A-stage systems. Also, the addition of state variables differentiating colloidal COD from suspended COD was necessary due to short hydraulic residence times in A-stage systems which do not allow for complete enmeshment and bioflocculation of these particles as occurs in conventional activated sludge systems (which have longer solid retention times and hydraulic retention times). It was necessary to add several processes (both stoichiometry and kinetic equations) to the original ASM1 model including heterotrophic growth on both soluble substrate fractions and bioflocculation of colloidal solids. How to properly quantify heterotrophic growth on SBs and SBf resulted in two separate approaches with respect to process kinetic equations. In one approach the SBf was metabolized preferentially over SBs which was only utilized when SBf was not available. This is referred to as the Diauxic Model. In the other approach SBf and SBs were metabolized simultaneously, and this is referred to as the Dual Substrate Model. The Dual Substrate Model calibrated slightly better than the Diauxic Model for one of the two available pilot studies data sets (the other set was used for model verification). The Dual Substrate A-stage model was used to describe the effects of varying specific operating parameters including solids retention time (SRT), dissolved oxygen (DO), influent COD and temperature on the effluent COD:N ratio. The effluent COD:N ratio target was based on its suitability for a downstream nitrite shunt (i.e. nitritation/denitritation) process. In the downstream process the goal is to eliminate nitrite oxidizing bacteria (NOB) from the reactor while selecting for ammonia oxidizing bacteria (AOB). The results showed that a low SRT ((<)0.25 d) can produce high effluent substrates (SB and CB), and elevated COD:N ratios consistent with NOB out-selection downstream, the HRAS model was able to predict the measured higher fraction of CB in the A-stage effluent at lower SRTs and DO concentrations, and to achieve the benefits of operating an A-stage process, while maintaining an effluent COD:N ratio suitable for a downstream nitritation/denitritation process, an A-stage SRT in the range of 0.1 to 0.25 d should be maintained.This research also included an analysis of A-stage pilot data using stoichiometry to determine the bio-products formed from soluble substrate removed in an A-stage reactor. The results were used to further refine the process components and stoichiometric parameters to be used in the A-stage dynamic computer model, which includes process mechanisms for flocculation and enmeshment of particulate and colloidal substrate, hydrolysis, production of extracellular polymeric substances (EPS) and storage of soluble biodegradable substrate. Analysis of pilot data and simulations with the dynamic computer model implied (indirectly) that storage products were probably significant in A-stage COD removal.
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Date Issued
2015
Identifier
CFE0005677, ucf:50161
Format
Document (PDF)
PURL
http://purl.flvc.org/ucf/fd/CFE0005677
An Assessment of Biosorption Activated Media for the Removal of Pollutants in Up-Flow Stormwater Treatment Systems
Title
An Assessment of Biosorption Activated Media for the Removal of Pollutants in Up-Flow Stormwater Treatment Systems.
Creator
Hood, Andrew, Randall, Andrew, Wanielista, Martin, Chopra, Manoj, O'Reilly, Andrew, Moore, Sean, University of Central Florida
Abstract / Description
Nitrogen and phosphorus are often the limiting nutrients for marine and freshwater systems respectively. Additionally, stormwater often contains elevated levels of pathogens which can pollute the receiving water body and impact reuse applications [1-4]. The reduction of limiting nutrients and pathogens is a common primary target for stormwater best management practices (BMPs) [5]. Traditional BMPs, such as retention/detention treatment ponds require large footprints and may not be practical...
Show moreNitrogen and phosphorus are often the limiting nutrients for marine and freshwater systems respectively. Additionally, stormwater often contains elevated levels of pathogens which can pollute the receiving water body and impact reuse applications [1-4]. The reduction of limiting nutrients and pathogens is a common primary target for stormwater best management practices (BMPs) [5]. Traditional BMPs, such as retention/detention treatment ponds require large footprints and may not be practical in ultra-urban environments where above ground space is limited. Upflow filters utilizing biosorption activated media (BAM) that can be placed underground offer a small footprint alternative. Additionally, BAM upflow filters can be installed at the discharge point of traditional stormwater ponds to provide further treatment. This research simulated stormwater that had already been treated for solids removal; thus, most of the nutrients and solids in the influent were assumed to be as non-settable suspended solids or dissolved solids. Three different BAM mixtures in an upflow filter configuration were compared for the parameters of nitrogen, phosphorus, total coliform, E. coli, and heterotrophic plate count (HPC). Additionally, genetic testing was conducted using Polymerase Chain Reaction (PCR), in conjunction with a nitrogen mass balance, to determine if Anammox was a significant player in the nitrogen removal. The columns were run at both 22-minute and 220-minute Empty Bed Contact Times (EBCTs). All the BAM mixtures analyzed were shown to be capable at the removal of nitrogen, phosphorus, and total coliform during both the 22-minute and 220-minute EBCTs, with BAM #1 having the highest removal performance for all three parameters during both EBCTs. All BAM mixtures experienced an increase in HPC. Additionally, PCR analysis confirmed the presence of Anammox in the biofilm and via mass balance it was determined that the biological nitrogen removal was due to Anammox and endogenous denitrification with Anammox being a significant mechanism.
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Date Issued
2019
Identifier
CFE0007817, ucf:52875
Format
Document (PDF)
PURL
http://purl.flvc.org/ucf/fd/CFE0007817