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- Title
- RETROSPECTIVE ANALYSIS OF SCREENING PATTERNS IN CIRRHOTIC PATIENTS WITH HEPTOCELLULAR CARCINOMA.
- Creator
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Scott-Castell, Shelly-Ann, Chase, Susan, University of Central Florida
- Abstract / Description
-
ABSTRACT The incidence of hepatocellular carcinoma (HCC) in cirrhotic patients is increasing worldwide. Cirrhotic patients are recommended by the American Association for the Study of Liver Disease (AASLD) to receive HCC screening and surveillance every 6 months to a year. The purpose of this study was to identify the current screening and surveillance patterns for cirrhotic patients with HCC in clinical practice. Hepatocellular carcinoma can be detected by radiological studies in addition to...
Show moreABSTRACT The incidence of hepatocellular carcinoma (HCC) in cirrhotic patients is increasing worldwide. Cirrhotic patients are recommended by the American Association for the Study of Liver Disease (AASLD) to receive HCC screening and surveillance every 6 months to a year. The purpose of this study was to identify the current screening and surveillance patterns for cirrhotic patients with HCC in clinical practice. Hepatocellular carcinoma can be detected by radiological studies in addition to laboratory testing. It is important to implement the AASLD screening guidelines, as early identification might decrease the mortality rate of patients with cirrhosis and HCC. The research question guiding this study was: What are the screening patterns of cirrhotic patients diagnosed with cirrhosis and HCC that have been referred to the Hepatology Division? A retrospective, descriptive, cross-sectional design was used for this study. Data were collected from subjects who were referred to a Specialty Hepatology Division for evaluation and treatment. Approval was obtained from the IRB. Cirrhotic patients diagnosed with HCC meeting the inclusion and exclusion criteria were used in this study. The aim of the study was to identify the clinical patterns of practitioners screening for HCC in cirrhotic patients. Validity and reliability for the data collection tool was not established. Variables that were studied included demographic data, etiology of cirrhosis, type of HCC screening, time increments of screening, and size of tumor at the time of diagnosis. The data were analyzed with the use of crosstabs, frequency, and correlation statistics. Despite the recommended HCC screening and surveillance guidelines cirrhotic patients were not screened. The different screening patterns that were identified were none, sporadic, and annual (every 6 months to 1 year). The patterns differed by the practitioner managing the patient. Also, cirrhosis was diagnosed late in the disease process, although many of the patients are followed by gastroenterologists. It can be assumed that the late diagnosis of cirrhosis was another factor that was preventing the implementation of HCC screening and surveillance. Implications for practice were identified. Practitioners are responsible for performing HCC screening and surveillance of cirrhotic patients based on the recommended guidelines of the AASLD for the management of cirrhotic patients and the detection of small lesions. Only 33% of the patients were screened with the use of ultrasound, and 43% were screened with alpha-fetoprotein. The lesions that were diagnosed were larger in the non-screened patients than the screened patients. The Hepatology Division was the only setting that was screening the patientsÂÂ' based on the recommended guidelines. The recommendation based on the results of this study is for all cirrhotic patients to be managed by hepatology services if one is available.
Show less - Date Issued
- 2010
- Identifier
- CFE0003386, ucf:48468
- Format
- Document (PDF)
- PURL
- http://purl.flvc.org/ucf/fd/CFE0003386
- Title
- TEMPORARY BARRIERS REDUCE RUBBERNECKING AND EXTERNAL DISTRACTION ON ROADWAYS.
- Creator
-
Colon, Nicholas, Mouloua, Mustapha, University of Central Florida
- Abstract / Description
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The purpose of the current study was to empirically examine the effects of accident scenes on eye movement as well as driving behavior. Fifty-four participants drove in a driving simulator wearing a head-mounted eye-tracker in three experimental drives, one of which had an accident scene. The participants were put into one of three different conditions (no barrier, partial barrier, or full barrier). The results showed significant main effects of distraction (accident vs. no accident) on dwell...
Show moreThe purpose of the current study was to empirically examine the effects of accident scenes on eye movement as well as driving behavior. Fifty-four participants drove in a driving simulator wearing a head-mounted eye-tracker in three experimental drives, one of which had an accident scene. The participants were put into one of three different conditions (no barrier, partial barrier, or full barrier). The results showed significant main effects of distraction (accident vs. no accident) on dwell frequency and duration, average speed, and root mean square error of the steering wheel angle during the drive with the accident scenes. In addition, the results also showed significant interaction effects between distraction and type of barrier (no, partial, or full) on dwell frequency and duration. The full barrier condition had the biggest effect on decreasing dwell duration and frequency. The findings support the Salience Effort Expectancy Value (SEEV) model of attention and previous research stating objects high in salience attract attention (Wickens & Horrey, 2008; Itti & Koch, 2000). These findings also support previous research by Mayer, Caird, Milloy, Percival, & Ohlhauser (2010) stating that drivers drive in the safest manner (lowest passing speed) when an emergency vehicles are present with the emergency lights on. Temporary barriers could be used to help decrease the effects of rubbernecking on highways when an accident scene is present (Masinick & Teng, 2004; Potts, Harwood, Hutton, & Kinzel, 2010)
Show less - Date Issued
- 2013
- Identifier
- CFH0004331, ucf:45059
- Format
- Document (PDF)
- PURL
- http://purl.flvc.org/ucf/fd/CFH0004331
- Title
- WHAT ARE THE MOST COMMONLY USED TOOLS TO SCREEN DEPRESSION IN HIV-INFECTED GAY AND BISEXUAL MEN?.
- Creator
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Isner, Michael, Blackwell, Christopher, University of Central Florida
- Abstract / Description
-
Gay and bisexual men infected with human immunodeficiency virus (HIV) comprise a sizable, medically vulnerable population. Depression is the most commonly experienced mental health disorder affecting this group of people, lending itself to a host of risks associated with depression. As screening of depression in this population can be challenging, it is vital that clinicians have the best available tools and guidelines to detect depressive symptomology. This focused, comprehensive review of...
Show moreGay and bisexual men infected with human immunodeficiency virus (HIV) comprise a sizable, medically vulnerable population. Depression is the most commonly experienced mental health disorder affecting this group of people, lending itself to a host of risks associated with depression. As screening of depression in this population can be challenging, it is vital that clinicians have the best available tools and guidelines to detect depressive symptomology. This focused, comprehensive review of the literature examined current data describing the clinical instruments used to detect depressive symptoms in HIV-infected gay and bisexual men. The aim of this analysis was to seek out which instruments were the most widely and successfully employed for this population. An initial search using EBSCOhost and associated databases CINAHL, MEDLINE, PsycINFO, and Health and Psychosocial Instruments alongside inclusion and exclusion criteria found 1,899 articles. Results were narrowed using additional inclusion and exclusion criteria and relevancy, yielding a total of 13 articles for review. The findings of this review suggest screening of depressive symptoms in HIV-infected gay and bisexual men was most successful using the CES-D, the BSI-18, and the BDI. Health care providers should have an understanding of the importance in assessing this population for depression and have access to the best possible tools to do so.
Show less - Date Issued
- 2017
- Identifier
- CFH2000205, ucf:45928
- Format
- Document (PDF)
- PURL
- http://purl.flvc.org/ucf/fd/CFH2000205
- Title
- TWO ESSAYS ON SCREENING STRATEGIES.
- Creator
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Ganesh Pillai, Rajani, Xin He, Raj Echambadi, University of Central Florida
- Abstract / Description
-
Consumers form consideration sets by screening from all available alternatives. Consumers typically utilize one of two types of screening strategies: an exclusion screening strategy wherein alternatives not worthy of further consideration are rejected or an inclusion strategy wherein worthy alternatives are selected for further evaluation. Extant literature has documented the important role played by screening strategies in decision making. However, there is very limited understanding of when...
Show moreConsumers form consideration sets by screening from all available alternatives. Consumers typically utilize one of two types of screening strategies: an exclusion screening strategy wherein alternatives not worthy of further consideration are rejected or an inclusion strategy wherein worthy alternatives are selected for further evaluation. Extant literature has documented the important role played by screening strategies in decision making. However, there is very limited understanding of when and why consumers may employ one screening strategy over the other as well the impact of the screening strategy for decision accuracy. This dissertation attempts to study the antecedent and consequence of screening strategies. Essay 1 in this dissertation, investigates the role of consumers' perceived uncertainty on the choice of screening strategy. Four studies in this essay show that when consumers are highly uncertain they are more likely to choose exclusion screening strategy; whereas when they are less uncertain they are more likely to use inclusion screening. Mediation analyses in Studies 1 and 2 show that the choice of screening strategy is primarily driven by perceived accuracy of the strategy. Study 3 demonstrates that the effect of uncertainty on the choice of screening strategy is moderated by consideration set size. When uncertain consumers form smaller sets they are more likely to use exclusion screening, but this relationship flips when they form larger consideration sets. Finally, external validity for the relationship between uncertainty and choice of screening strategy is demonstrated in Study 4 using the popular TV game show Who Wants to be a Millionaire? Essay two in this dissertation, investigates the role of perceived uncertainty and consideration set size on the relationship between screening strategy and objective accuracy of the decision. Utilizing an experimental study with an actual choice task, I demonstrate that perceived uncertainty moderates the screening strategy-decision accuracy relationship. Further, this interactive relationship is contingent on consideration set sizes. Whereas consumers with high perceived uncertainty make higher quality decisions with inclusion while forming smaller consideration sets, their decision quality is higher with exclusion when forming larger sets. Likewise, while consumers with low perceived uncertainty make more accurate decisions with exclusion when forming smaller sets, the accuracy of their decisions increases with inclusion when forming larger sets. This dissertation contributes to literature on screening strategies by explicating perceived uncertainty as a critical factor that leads to consumers preferring one screening strategy versus the other. Furthermore, it adds to our understanding of an important consequence of using screening strategies – decision accuracy.
Show less - Date Issued
- 2009
- Identifier
- CFE0002927, ucf:48001
- Format
- Document (PDF)
- PURL
- http://purl.flvc.org/ucf/fd/CFE0002927
- Title
- ACCURACY OF PARENTAL REPORT ON PHONOLOGICAL INVENTORIES OF TODDLERS.
- Creator
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Teske, Kristin, Carson, Cecyle, University of Central Florida
- Abstract / Description
-
Considering the diminishing availability of professional resources, increasing costs, and time requirements involved in early childhood mass screenings, parents are an essential source of information. In this study, the Survey of Speech Development (SSD) (Perry-Carson & Steel, 2001; Steel, 2000) was used to determine the accuracy of parents in reporting the speech sound inventories of their toddlers. Parents of 30 children, who were between the ages of 27 to 33 months old, completed the SSD...
Show moreConsidering the diminishing availability of professional resources, increasing costs, and time requirements involved in early childhood mass screenings, parents are an essential source of information. In this study, the Survey of Speech Development (SSD) (Perry-Carson & Steel, 2001; Steel, 2000) was used to determine the accuracy of parents in reporting the speech sound inventories of their toddlers. Parents of 30 children, who were between the ages of 27 to 33 months old, completed the SSD prior to a speech and language assessment session. Based on assessment results, the children were classified as normal developing or language delayed. A 20-minute play interaction between the parent and child was recorded during the assessment and was transcribed later for analysis. Speech sounds (consonants) were coded as present or absent and comparisons were made between the parents results on the SSD and data from the 20-minute speech sample. A point-by-point reliability analysis of the speech sounds on the SSD compared to those produced in the speech sample revealed an overall parental accuracy of 75%. Further, no differences were found between parent reports and transcribed accounts for total number of different consonants. This was true for parents of both language delayed and language normal toddlers. Results suggest that if given a systematic means of providing information, parents are a reliable source of information regarding sounds their toddlers produce.
Show less - Date Issued
- 2005
- Identifier
- CFE0000676, ucf:46543
- Format
- Document (PDF)
- PURL
- http://purl.flvc.org/ucf/fd/CFE0000676
- Title
- THE EFFECTIVENESS OF FUNCTIONAL MOVEMENT SCREENING TESTING IN PREVENTION OF ANTERIOR CRUCIATE LIGAMENT INJURIES IN WOMEN'S COLLEGIATE SOCCER.
- Creator
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Ferrara, Morgan P, Fisher, Thomas, University of Central Florida
- Abstract / Description
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The Functional Movement Screen (FMS), invented in 1995, has been adopted among Division One sports programs across the country. Being a women's soccer player at the University of Central Florida (UCF), this particular topic had been of interest for years. The FMS is a series of seven tests evaluated at the beginning and end of each season. The UCF team's preventative rehabilitation was based upon the measurements from the FMS testing. The team engaged in preventative rehabilitation three...
Show moreThe Functional Movement Screen (FMS), invented in 1995, has been adopted among Division One sports programs across the country. Being a women's soccer player at the University of Central Florida (UCF), this particular topic had been of interest for years. The FMS is a series of seven tests evaluated at the beginning and end of each season. The UCF team's preventative rehabilitation was based upon the measurements from the FMS testing. The team engaged in preventative rehabilitation three times a week. Each year of my membership, the team of 28 to 30 players had no less than two anterior cruciate ligament tears each season. This research explored the effectiveness of the Functional Movement Screening, and its' predictive ability regarding injury to possibly prevent future injuries. The purpose of this study was to review literature of multiple studies exploring the Functional Movement Screen, the anterior cruciate ligament, and, specifically, the recent spike in women's collegiate soccer injuries. This study also examined and included findings from five years of FMS scoring data from the UCF women's soccer team. The study consisted of 43 participants, 29 in the control group and 14 in the test group (those who suffered and ACL tear). They were females, ages 18-23, and of fit manner. Multivariate analysis, independent and dependent T-Tests, and Leven's test ran these data. This study also investigated the reliability of the Functional Movement Screen and analyzed data about anterior cruciate ligament injuries among women's collegiate soccer players. Recommendations for future protocols and implications for coaches, trainers, and women soccer players are provided.
Show less - Date Issued
- 2018
- Identifier
- CFH2000297, ucf:45774
- Format
- Document (PDF)
- PURL
- http://purl.flvc.org/ucf/fd/CFH2000297
- Title
- SCREENING FOR ANTICANCER AGENTS TO INHIBIT MITOTIC KINASES AND PROLIFERATION OF METASTATIC PROSTATE CANCER CELLS.
- Creator
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Nguyen, Khoa, Chakrabarti, Ratna, University of Central Florida
- Abstract / Description
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Current treatments for prostate cancer (PCa) are marred with high relapse frequency and development of progressively aggressive cancers; developing new treatment options for PCa remains crucial. In this project, a series of synthetic compounds based on natural products will be screened to identify inhibitors for Aurora-A kinase (Aur-A). Aur-A facilitates centrosome separation and bipolar spindle formation during mitosis. Aur-A is overexpressed in metastatic PCa cells, and is a good candidate...
Show moreCurrent treatments for prostate cancer (PCa) are marred with high relapse frequency and development of progressively aggressive cancers; developing new treatment options for PCa remains crucial. In this project, a series of synthetic compounds based on natural products will be screened to identify inhibitors for Aurora-A kinase (Aur-A). Aur-A facilitates centrosome separation and bipolar spindle formation during mitosis. Aur-A is overexpressed in metastatic PCa cells, and is a good candidate for targeted therapies. Compound libraries are designed using natural compounds that contain simple structural elements as starting points for developing drug like libraries. High-throughput screening of these libraries will be used to identify potent antimitotic agents that selectively affect cancer cells but not normal cells. A combination of in vitro protein assays � quantifying protein activity � cell-based assays � measuring cell growth and proliferation � and cell-reporter assays � to determine which metabolic pathway the compound affects � were used to identify potential inhibitors. Through these methods, we have identified several compounds, with special consideration to thiazole piperazine compounds, to successfully inhibit proliferation of metastatic PCa cells.
Show less - Date Issued
- 2016
- Identifier
- CFH2000103, ucf:45549
- Format
- Document (PDF)
- PURL
- http://purl.flvc.org/ucf/fd/CFH2000103
- Title
- A Behavioral Model of Law Enforcement Applicant Characteristics Derived from a Simulated Cheating Task: Implications for Pre-Employment Hiring Practices.
- Creator
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Montaquila, Julian, Caulkins, Bruce, Wiegand, Rudolf, Teo, Grace, Beever, Jonathan, University of Central Florida
- Abstract / Description
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Recently, numerous high-profile instances of police misconduct and corruption have been thrust into the national spotlight. Hiring police officers who will act with integrity and not betray public trust remains essential. The present research experimentally examines this phenomenon by evaluating pre-employment assessment results against applicant performance on a simulated cheating task (i.e., The Dots Task) in order to derive information to improve contemporary pre-employment screening and...
Show moreRecently, numerous high-profile instances of police misconduct and corruption have been thrust into the national spotlight. Hiring police officers who will act with integrity and not betray public trust remains essential. The present research experimentally examines this phenomenon by evaluating pre-employment assessment results against applicant performance on a simulated cheating task (i.e., The Dots Task) in order to derive information to improve contemporary pre-employment screening and selection models. Four case examples are presented which depict malicious actors who possessed privileged access, assumed no one would ever scrutinize their activities, and attempted to leverage a lack of oversight for their personal benefit. A literature review of previous research findings is presented, and results from the current study are discussed. Spearman correlation analyses consistently indicated that participants who cheated were predisposed to moral disengagement via advantageous comparison. Participants who left all or part of their monetary award were less prone to general moral disengagement, particularly displacement of responsibility, while the opposite effect was observed for participants who took more than their earned award. Impression management was positively associated with stealing extra money, and cheating was more common among participants with elevated distorted thought patterns, including obsessional thinking, paranoid ideation, and alienation/perceptual distortion. Stepwise linear multiple regression analyses further substantiated the relationship between cheating and both distorted thought patterns and impression management, as well as provided evidence that (1) internalizing morality as part of one's self-identity and (2) warmth act as protective factors against cheating behavior. Positive relationships between cheating and distortion of consequences were also present within multiple regression analyses. Behavioral models produced from stepwise linear multiple regression analyses offer the potential to predict the likelihood and severity of cheating behavior that an individual may be predisposed to commit based upon their pre-employment assessment data, thereby enhancing pre-employment screening and selection decisions.
Show less - Date Issued
- 2018
- Identifier
- CFE0007409, ucf:52714
- Format
- Document (PDF)
- PURL
- http://purl.flvc.org/ucf/fd/CFE0007409
- Title
- CO Florida 2012, A MOVES-Based, Near-Road, Screening Model.
- Creator
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Ritner, Mark, Cooper, Charles, Radwan, Ahmed, Randall, Andrew, University of Central Florida
- Abstract / Description
-
Citizens in the United States are fortunate to have an excellent system of roadways and the affluence with which to afford automobiles. The flexibility of travel on demand for most allows for a variety of lifestyles, assists with conducting business, and contributes to the feeling of freedom that most citizens enjoy. The current vehicle fleet, which is primarily powered by internal combustion engines burning fossil fuels, does however contribute to the deterioration of air quality. This...
Show moreCitizens in the United States are fortunate to have an excellent system of roadways and the affluence with which to afford automobiles. The flexibility of travel on demand for most allows for a variety of lifestyles, assists with conducting business, and contributes to the feeling of freedom that most citizens enjoy. The current vehicle fleet, which is primarily powered by internal combustion engines burning fossil fuels, does however contribute to the deterioration of air quality. This effect is particularly significant in metropolitan areas. Motor vehicle exhausts contain several combustion bi-products that pose harmful effects to the environment and human health, in particular. The United States Environmental Protection Agency (EPA) and the Federal Highway Administration (FHWA) have selected carbon monoxide (CO) as the air pollutant on which it has based its guidelines for assessing potential air quality impacts from roadway construction (EPA 1992).The design of roadway networks must consider traffic flows, Level of Service (LOS), cost, and National Ambient Air Quality Standards (NAAQS) requirements. In light of the environmental standards it is necessary to model to estimate potential future near-road concentrations of CO. This modeling has two aspects, first determining the rate of pollutant emissions, and second determining how those pollutants disperse near the road. Obtaining a precise, realistic estimate of the near-road CO concentrations under a wide variety of weather and traffic patterns is a potentially huge undertaking. With budgetary constraints in mind, the development of a screening model is appropriate. CO Florida 2012 (COFL2012) is such a model that uses conservative assumptions to predict worst-case, near-road CO concentration. Projects that pass a COFL2012 model run do not require additional air quality modeling. Projects that fail a COFL2012 model run, however, may still be viable, but will require additional, detailed modeling and possibly project modifications.COFL2012 uses tables of emission factors (EFs) that were derived from numerous runs of the EPA's MOtor Vehicle Emission Simulator (MOVES2010a), which is indicated as the preferred model for near-road modeling of CO.(EPA 2009) COFL2012 then inputs the EFs, along with assumed link configurations, geographical assumptions, and user-inputted traffic information into input files that are run through CAL3QHC Version 2.0 (CAL3QHC2), the EPA's approved near-road dispersion model (EPA 1995).COFL2012 is a brand new Florida CO screening model, written from scratch. This author has written the computer code for COFL2012 in Visual Basic, using Microsoft Visual Studios 2010. Visual Studios utilizes the .net Framework 4. COFL2012 is easy to learn, quick to operate, and has been written to allow for future updates simply and easily, whenever the EPA releases updates to the databases that feed MOVES2010a.
Show less - Date Issued
- 2012
- Identifier
- CFE0004233, ucf:49011
- Format
- Document (PDF)
- PURL
- http://purl.flvc.org/ucf/fd/CFE0004233
- Title
- IDENTIFYING PATIENTS AT RISK FOR OBSTRUCTIVE SLEEP APNEA IN PRIMARY HEALTH CARE: CAN OBESITY IN COMBINATION WITH OTHER HIGH-RISK DIAGNOSES BE USED FOR SCREENING PURPOSES?.
- Creator
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Lima, Clelia, Norris, Anne, University of Central Florida
- Abstract / Description
-
Obstructive sleep apnea (OSA) affects about 15 million adults in the United States, and is an independent risk factor for all-cause mortality. The under-diagnosing of OSA has been linked to the inadequate screening by primary care practitioners (PCPs). Existing screening tools are not widely used by PCPs possibly due to time constraints they experience as providers. This study demonstrates how common high-risk diagnoses (obesity, hypertension, diabetes mellitus type 2, dyslipidemia,...
Show moreObstructive sleep apnea (OSA) affects about 15 million adults in the United States, and is an independent risk factor for all-cause mortality. The under-diagnosing of OSA has been linked to the inadequate screening by primary care practitioners (PCPs). Existing screening tools are not widely used by PCPs possibly due to time constraints they experience as providers. This study demonstrates how common high-risk diagnoses (obesity, hypertension, diabetes mellitus type 2, dyslipidemia, arrhythmia, and coronary artery disease) can be used to help PCPs identify adult patients at risk for OSA. Unlike other screening tools, these diagnoses are easy to identify in a routine visit. This study was a retrospective chart review that used a random sample of 220 electronic health records. Seventy percent of the sample was positive for OSA, 69% had obesity, and 33% had two or more high-risk diagnoses. The setting of this study was six sleep centers located in five cities in Central Florida. Logistic regression was used to analyze the data to determine interaction among variables and odds ratios. The variables "obesity" and "two or more high-risk diagnoses" had significant effects on the likelihood of being diagnosed with OSA independently of each other (odds ratio of 4.2 and 4.3 respectively; p<.001). However, there was no significant interaction between these two variables (p=.56). The predictive value for an OSA diagnosis using "obesity" was 83%, and it was 88% using "two or more high-risk diagnoses." These findings argue for the use of high-risk diagnoses to identify patients at risk for OSA. PCPs are in an ideal position to increase the number of patients screened and treated for OSA because they routinely see patients with these diagnoses in their practices. Proper diagnosis and treatment of OSA has the potential to improve patients' outcomes and their quality of life.
Show less - Date Issued
- 2011
- Identifier
- CFE0003620, ucf:48863
- Format
- Document (PDF)
- PURL
- http://purl.flvc.org/ucf/fd/CFE0003620
- Title
- THE SEARCH FOR TRUTH IN THE DIGITAL CINEMATIC SPACE AND GREEN SCREEN PERFORMANCES.
- Creator
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Dalton, Kade, Shults, Kate, University of Central Florida
- Abstract / Description
-
With the advent of the blockbuster and its visual effects, film has grown to accept these effects and the technology behind them, namely green screen mattes, as cornerstones to the post-production process. The propensity of green screen in all types of productions, especially those involving actors and their performances, raises questions about the methodology and workflow behind its common practice. Using real-life environments and people to create narrative scenes, this project explores the...
Show moreWith the advent of the blockbuster and its visual effects, film has grown to accept these effects and the technology behind them, namely green screen mattes, as cornerstones to the post-production process. The propensity of green screen in all types of productions, especially those involving actors and their performances, raises questions about the methodology and workflow behind its common practice. Using real-life environments and people to create narrative scenes, this project explores the utilization of matte backgrounds to inform the rehearsal and performance aspects of cinematic story-telling.
Show less - Date Issued
- 2014
- Identifier
- CFH0004714, ucf:45409
- Format
- Document (PDF)
- PURL
- http://purl.flvc.org/ucf/fd/CFH0004714
- Title
- IDENTIFICATION OF SMALL MOLECULES THAT INHIBIT PROSTATE CANCER CELL PROLIFERATION.
- Creator
-
Zelaya, Rainel, Chakrabarti, Ratna, University of Central Florida
- Abstract / Description
-
Prostate cancer is the second most often diagnosed cancer and internationally the sixth foremost cause of cancer death in males, as of 2011. Within the United States it is the most common form of cancer in men with 186,000 new cases and with an overall 28,600 deaths in 2008, and it is the second leading kind of cancer-related death in men. The widespread threat that prostate cancer poses against men across the globe cannot be understated, and its initiation and progression must be understood...
Show moreProstate cancer is the second most often diagnosed cancer and internationally the sixth foremost cause of cancer death in males, as of 2011. Within the United States it is the most common form of cancer in men with 186,000 new cases and with an overall 28,600 deaths in 2008, and it is the second leading kind of cancer-related death in men. The widespread threat that prostate cancer poses against men across the globe cannot be understated, and its initiation and progression must be understood in order to truly comprehend its implicated risks and possible forms of treatment. As its name implies, prostate cancer is a form of cancer that develops in the prostate gland located in the male reproductive system. Its progress starts when standard semen-secreting prostate gland cells mutate into cancer cells. Although its developments may start at the prostate gland, cancer cells may metastasize to other parts of the body through circulation systems such as the lymph nodes. The main sites of metastasis for prostate cancer include the adrenal gland,the bones, the liver and the lungs. Although there are treatments available for prostate cancer, there is no definitive cure. The primary goal of this project was to find an alternative form of treatment, which is what will be necessary to combat this cancer.
Show less - Date Issued
- 2014
- Identifier
- CFH0004595, ucf:45228
- Format
- Document (PDF)
- PURL
- http://purl.flvc.org/ucf/fd/CFH0004595
- Title
- PERCEPTION AND DISPLAYS FOR TELEOPERATED ROBOTS.
- Creator
-
Upham Ellis, Linda, Sims, Valerie, University of Central Florida
- Abstract / Description
-
In remote or teleoperational tasks involving humans and robots, various aspects of the remote display system may greatly influence the individual's interactions with the teleoperated entity. This dissertation examined various configurations of display systems on several measures of operator performance, physiological states, and perceptions of the task. Display configurations included altering the camera placement (attached to the robot or placed overhead), screen orientation (horizontal...
Show moreIn remote or teleoperational tasks involving humans and robots, various aspects of the remote display system may greatly influence the individual's interactions with the teleoperated entity. This dissertation examined various configurations of display systems on several measures of operator performance, physiological states, and perceptions of the task. Display configurations included altering the camera placement (attached to the robot or placed overhead), screen orientation (horizontal or vertical), and screen size (small or large). Performance was measured in terms of specific task goals, accuracies, strategies, and completion times. Physiological state was assessed through physiological markers of arousal, specifically heart rate and skin conductance. Operator perception of the task was measured with a self-reported perception of workload and frustration. Scale model live simulation was used to create a task driven environment to test the display configurations. Screen size influenced performance on complex tasks in mixed ways. Participants using a small screen exhibited better problem solving strategies in a complex driving task. However, participants using the large screen exhibited better driving precision when the task required continual attention. These findings have value in design decisions for teleoperated interfaces where the advantages and disadvantages of screen size must be considered carefully. Orientation of the visual information seems to have much less impact on the operator than the source of the information, though it was an important factor of the display system when taken together with screen size and camera view. Results show strong influence of camera placement on many of the performance variables. Interestingly, the participants rated a higher frustration in the overhead condition, but not a higher task load, indicating that while they realized that the task was frustrating and perhaps they could have done better, they did not recognize the task as overloading. This was the case even though they took longer to complete the task and experienced more errors related to turning in the overhead camera condition. This finding may indicate a potential danger for systems in which the operator is expected to recognize when he or she is being overloaded. This type of performance decrease due to added frames of reference may be too subtle to register in the operator's self awareness
Show less - Date Issued
- 2008
- Identifier
- CFE0002330, ucf:47818
- Format
- Document (PDF)
- PURL
- http://purl.flvc.org/ucf/fd/CFE0002330
- Title
- A Solid Phase Assay for Topoisomerase I interfacial Poisons and Catalytic Inhibitors.
- Creator
-
Cyril Sagayaraj, Vidusha, Muller, Mark, Zhao, Jihe, Chakrabarti, Debopam, University of Central Florida
- Abstract / Description
-
We report a mechanism based screening technique to rapidly identify eukaryotic topoisomerase I targeting agents. The method is based on genetic tagging of topoisomerase I to immobilize the enzyme on a solid surface in a microtiter well format. DNA is added to the wells and retained DNA is detected by Picogreen fluorescence. Compounds that result in an increase in Picogreen staining represent potential topoisomerase interfacial poisons while those that reduce fluorescence report catalytic...
Show moreWe report a mechanism based screening technique to rapidly identify eukaryotic topoisomerase I targeting agents. The method is based on genetic tagging of topoisomerase I to immobilize the enzyme on a solid surface in a microtiter well format. DNA is added to the wells and retained DNA is detected by Picogreen fluorescence. Compounds that result in an increase in Picogreen staining represent potential topoisomerase interfacial poisons while those that reduce fluorescence report catalytic inhibitors; therefore, the solid phase assay represents a 'bimodal' readout that reveals mechanisms of action. The method has been demonstrated to work with known interfacial poisons and catalytic inhibitors. In addition to specific topoisomerase targeting drugs, the method also weakly detects other relevant anticancer agents, such as potent DNA alkylating and intercalating compounds; therefore, topoisomerase I HTS represents an excellent tool for searching and identifying novel genotoxic agents. This method is rapid, robust, economical and scalable for large library screens.
Show less - Date Issued
- 2011
- Identifier
- CFE0004473, ucf:49304
- Format
- Document (PDF)
- PURL
- http://purl.flvc.org/ucf/fd/CFE0004473
- Title
- Multi-target high-throughput screening assays for antimicrobial drug discovery.
- Creator
-
Grube, Christopher, Roy, Herve, Chakrabarti, Debopam, Moore, Sean, Koculi, Eda, University of Central Florida
- Abstract / Description
-
The rise of antibiotic resistant microbes (bacteria, fungi, and parasites), combined with the current void of new drugs entering the clinical setting, has created an urgent need for the discovery of new antimicrobials. High-throughput screening (HTS) assays represent a fast and cost-efficient method for identifying new therapeutic compounds and have been the longstanding gold standard for drug discovery. The focus of this dissertation is on the development and implementation of novel...
Show moreThe rise of antibiotic resistant microbes (bacteria, fungi, and parasites), combined with the current void of new drugs entering the clinical setting, has created an urgent need for the discovery of new antimicrobials. High-throughput screening (HTS) assays represent a fast and cost-efficient method for identifying new therapeutic compounds and have been the longstanding gold standard for drug discovery. The focus of this dissertation is on the development and implementation of novel methodologies to increase the throughput of target-based HTS by designing assays that allow multiple drug targets to be probed simultaneously. During my graduate studies, I developed three distinct HTS assays. In each of these assays, drug targets were incorporated into synthetic pathways obeying various reaction topologies (e.g., cyclical, parallel, or linear). Each of these reaction topologies conferred specific advantages and limitations to the individual assays. The first assay reconstitutes the bacterial tRNA-dependent pathway for lipid aminoacylation. This two-step pathway combines a tRNA aminoacylation step catalyzed by an aminoacyl-tRNA synthetase (aaRS), and a transferase step, which transfers the amino acid born by the tRNA onto membrane lipids. aaRSs are essential enzymes in all domains of life and represent longstanding drug targets in pathogenic species. The transferase reaction in the pathway is also an appealing drug target since it impacts the cellular permeability of antibiotics. Inhibitors of this reaction could dramatically increase the efficacy of existing therapeutics. The second assay I developed also targets aaRSs, but utilizes a parallel topology that permits the probing of the synthetic and editing activities of up to four aaRSs simultaneously. The third assay utilizes a linear topology that reconstitutes the entire purine salvage pathway from Plasmodium falciparum. Because parasites are unable to synthesize purines de novo, this pathway represents an appealing target for novel antimalarials. Pilot screens using this assay revealed inhibitors for multiple enzymes in the pathway, validating the design of the system. This body of work aims to shift the current paradigm of single-target systems that have historically dominated the HTS field, toward multi-target designs that can be used to more efficiently screen compound libraries against essential pathways in pathogenic microbes.
Show less - Date Issued
- 2019
- Identifier
- CFE0007642, ucf:52469
- Format
- Document (PDF)
- PURL
- http://purl.flvc.org/ucf/fd/CFE0007642
- Title
- Assessment of molecular interactions via magnetic relaxation: a quest for inhibitors of the anthrax toxin.
- Creator
-
Santiesteban, Oscar, Perez Figueroa, Jesus, Liao, Yi, Yestrebsky, Cherie, Hampton, Michael, Lambert, Stephen, University of Central Florida
- Abstract / Description
-
Anthrax is severe disease caused by the gram-positive Bacillus anthracis that can affect humans with deadly consequences. The disease propagates via the release of bacterial spores that can be naturally found in animals or can be weaponized and intentionally released into the atmosphere in a terrorist attack. Once inhaled, the spores become activated and the anthrax bacterium starts to reproduce and damage healthy macrophages by the release of the anthrax toxin. The anthrax toxin is composed...
Show moreAnthrax is severe disease caused by the gram-positive Bacillus anthracis that can affect humans with deadly consequences. The disease propagates via the release of bacterial spores that can be naturally found in animals or can be weaponized and intentionally released into the atmosphere in a terrorist attack. Once inhaled, the spores become activated and the anthrax bacterium starts to reproduce and damage healthy macrophages by the release of the anthrax toxin. The anthrax toxin is composed of three virulent factors: (i) anthrax protective antigen (APA), (ii) anthrax lethal factor (ALF), and (iii) anthrax edema factor (AEF) that work in harmony to effectuate the lethality associated with the disease. Out of the two internalized factors, ALF has been identified to play a critical role in cell death. Studies in animals have shown that mice infected with an anthrax strain lacking ALF survive the infection whereas when ALF is present the survivability of the mice is eliminated. Although the current therapy for anthrax is antibiotic treatment, modern medicine faces some critical limitations when combating infections. Antibiotics have proven very efficient in eliminating the bacterial infection but they lack the ability to destroy or inhibit the toxins released by the bacteria. This is a significant problem since ALF can remain active in the body for days after the infection is eliminated with no way of inhibiting its destructive effects. The use of inhibitors of ALF is an attractive method to treat the pathogenesis of anthrax infections. Over the last decade several inhibitors of the enzymatic activity of ALF have been identified. In order to identify inhibitors of ALF a variety of screening approaches such as library screenings, Mass Spectroscopy- based screenings and scaffold-based NMR screening have been used. Results from these screening have yielded mainly small molecules that can inhibit ALF in low micromolar to nanomolar concentrations. Yet, although valuable, these results have very little significance with regards to treating ALF in a real-life scenario since pharmaceutical companies are not willing to invest in further developing these inhibitors. Furthermore, the low incidence of inhalation anthrax, the lack of a market for an ALF inhibitor, and the expenses associated with the approval process of the FDA, have hindered the motivation of pharmaceutical companies to pursuit these kind of drugs. Therefore we have screened a small-molecule library of FDA approved drugs and common molecules in order to identify currently approved FDA drugs that can also inhibit ALF (Chapter III). The screening revealed that five molecules: sulindac, fusaric acid, naproxen, ketoprofen and ibuprofen bound to either ALF or APA with sulindac binding both. Additionally, we have developed a nanoparticle-based screening method that assesses molecular interactions by magnetic relaxation changes (Chapter II). Using this assay, we were able to accurately measure the dissociation constants of different interactions between several ligands and macromolecules. Moreover, we have used computational docking studies to predict the binding site of the identified molecules on the ALF or APA (Chapter IV). These studies predicted that two molecules sulindac and fusaric acid could be potential inhibitors of ALF since they bind at the enzymatic pocket. As a result, we tested the inhibitory potential of these molecules as well as that of the metabolic derivatives of sulindac (Chapter V). Results from these studies provided conclusive evidence that fusaric acid and sulindac were both strong inhibitors of ALF. Furthermore, the metabolic derivatives of sulindac, sulindac sulfide and sulindac sulfone also inhibited ALF. Overall, taking together these results we have discovered the alternate use of a currently used drug for the treatment of ALF pathogenesis.
Show less - Date Issued
- 2012
- Identifier
- CFE0004794, ucf:49745
- Format
- Document (PDF)
- PURL
- http://purl.flvc.org/ucf/fd/CFE0004794
- Title
- DEVELOPMENT OF A FLUORESCENT DRUG SCREENING PLATFORM FOR INHIBITORS OF MYCOBACTERIUM TUBERCULOSIS PROTEIN-PROTEIN INTERACTIONS.
- Creator
-
Versfeld, Zina, Rohde, Kyle, University of Central Florida
- Abstract / Description
-
Tuberculosis (TB) is a respiratory disease caused by Mycobacterium tuberculosis (Mtb) that kills around 1.3 million people annually. Multi-drug resistant TB (MDR-TB) strains are increasingly encountered, in part resulting from shortcomings of current TB drug regimens that last between six to nine months. Patients may stop taking the antibiotics during their allotted regimen, leading to drug resistant TB strains. Novel drug screening platforms are therefore necessary to find drugs effective...
Show moreTuberculosis (TB) is a respiratory disease caused by Mycobacterium tuberculosis (Mtb) that kills around 1.3 million people annually. Multi-drug resistant TB (MDR-TB) strains are increasingly encountered, in part resulting from shortcomings of current TB drug regimens that last between six to nine months. Patients may stop taking the antibiotics during their allotted regimen, leading to drug resistant TB strains. Novel drug screening platforms are therefore necessary to find drugs effective against MDR-TB. In order to discover compounds that target under-exploited pathways that may be essential only in vivo, the proposed screening platform will use a novel approach to drug discovery by blocking essential protein-protein interactions (PPI). In Mtb, PPI can be monitored by mycobacterial protein fragment complementation (M-PFC). This project will re-engineer the M-PFC assay to include the red fluorescent mCherry reporter for increased efficiency and sensitivity in high-throughput screening applications. To optimize the mCherry assay, we have developed fluorescent M-PFC reporter strains to monitor distinct PPI required for Mtb virulence: homodimerization of the dormancy regulator DosR. A drug screen will then identify novel compounds that inhibit this essential PPI. The screen will involve positional-scanning combinatorial synthetic libraries, which are made up of chemical compounds with varying side chains. This work will develop novel tools for TB drug discovery that could identify new treatments for the emerging world threat of MDR-TB.
Show less - Date Issued
- 2015
- Identifier
- CFH0004785, ucf:45369
- Format
- Document (PDF)
- PURL
- http://purl.flvc.org/ucf/fd/CFH0004785
- Title
- IDENTIFICATION OF POTENTIAL LEAD ANTIMALARIAL COMPOUNDS FROM MARINE MICROBIAL EXTRACTS.
- Creator
-
Carbonell, Abigail, Chakrabarti, Debopam, University of Central Florida
- Abstract / Description
-
Malaria, caused by the parasite Plasmodium falciparum, has a long history as a global health threat. The vector-borne disease causes millions of deaths yearly, especially in developing countries with tropical climates that facilitate transmission. Compounding the problem is the emergence of drug-resistant strains due to overuse of outdated treatments. New compounds with antiplasmodial activity are needed to be developed as effective drugs against malaria. The hypothesis for this project is...
Show moreMalaria, caused by the parasite Plasmodium falciparum, has a long history as a global health threat. The vector-borne disease causes millions of deaths yearly, especially in developing countries with tropical climates that facilitate transmission. Compounding the problem is the emergence of drug-resistant strains due to overuse of outdated treatments. New compounds with antiplasmodial activity are needed to be developed as effective drugs against malaria. The hypothesis for this project is that marine microorganisms have a high likelihood of yielding novel antiplasmodial chemotypes because of their high diversity, which has not yet been explored for antimalarial development. In this project, microbes harvested and fermented by the Harbor Branch Oceanographic Institute in Fort Pierce, Florida were explored as sources for antiplasmodial natural products. Using a SYBR Green I fluorescence-based assay, 1,000 microbial extracts were screened for inhibition of the multidrug-resistant Plasmodium falciparum strain Dd2. Dose-response analysis was performed on 46 fractions from isolates whose extracts demonstrated [greater-than or equal to] 70% inhibition of Dd2 at 1 [micro]g/mL. To evaluate cytotoxicity, the MTS cell viability assay was used to calculate IC50 of extracts from active isolates in NIH/3T3 embryonic mouse fibroblasts. Several extracts demonstrated low IC50 in Dd2 and high IC50 in 3T3, suggesting that they contain potential lead antimalarial compounds. Extracts with high selectivity indices (potent plasmodial inhibition with low mammalian toxicity) have been prioritized for dereplication, with the goal of identifying novel active components that can be developed as antimalarial drugs.
Show less - Date Issued
- 2013
- Identifier
- CFH0004332, ucf:45035
- Format
- Document (PDF)
- PURL
- http://purl.flvc.org/ucf/fd/CFH0004332
- Title
- Uncertainty treatment in performance based seismic assessment of typical bridge classes in United States.
- Creator
-
Mehdizadeh Nasrabadi, Mohammad, Mackie, Kevin, Catbas, Necati, Yun, Hae-Bum, Xanthopoulos, Petros, University of Central Florida
- Abstract / Description
-
Bridge networks are expensive and complex infrastructures and are essential components of today's transportation systems. Despite the advancement in computer aided modeling and increasing the computational power which is increasing the accessibility for developing the fragility curves of bridges, the complexity of the problem and uncertainties involved in fragility analysis of the bridge structures in addition to difficulties in validating the results obtained from the analysis requires...
Show moreBridge networks are expensive and complex infrastructures and are essential components of today's transportation systems. Despite the advancement in computer aided modeling and increasing the computational power which is increasing the accessibility for developing the fragility curves of bridges, the complexity of the problem and uncertainties involved in fragility analysis of the bridge structures in addition to difficulties in validating the results obtained from the analysis requires precaution in utilization of the results as a decision making tool. The main focus of this research is to address, study and treatment of uncertainties incorporated in various steps of performance based assessments (PBA) of the bridge structures. In this research the uncertainties is divided into three main categories. First, the uncertainties that come from ground motions time and frequency content alteration because of scarcity of the recorded ground motions in the database. Second, uncertainties associated in the modeling and simulation procedure of PBA, and third uncertainties originated from simplistic approach and methods utilized in the conventional procedure of PBA of the structures. Legitimacy of the scaling of ground motions is studied using the response of several simple nonlinear systems to amplitude scaled ground motions suites. Bias in the response obtained compared to unscaled records for both as recorded and synthetic ground motions.Results from this section of the research show the amount of the bias is considerable and can significantly affect the outcome of PBA. The origin of the bias is investigated and consequently a new metric is proposed to predict the bias induced by ground motion scaling without nonlinear analysis. Results demonstrate that utilizing the predictor as a scaling parameter can significantly reduce the bias for various nonlinear structures. Therefore utilizing the new metric as the intensity measuring parameter of the ground motions is recommended in PBA. To address the uncertainties associated in the modeling and simulation, MSSS concrete girder bridge class were selected due to the frequency of the construction in USCS region and lack of seismic detailing. A large scale parameters screening study is performed using Placket-Burman experimental design that considers a more complete group of parameters to decrease the computational expense of probabilistic study of the structure's seismic response. Fragility analysis for MSSS bridge is performed and the effect of removing the lesser important parameters the probabilistic demand model was investigated. This study reveals parameters reduction based on screening study techniques can be utilized to increase efficiency in fragility analysis procedure without compromising the accuracy of the outcome. The results from this study also provides more direct information on parameter reduction for PBA as well as provide insight into where future investments into higher fidelity finite element and constitutive models should be targeted. Conventional simplistic PBA approach does not account for the fundamental correlation between demand and capacity models. A more comprehensive PBA approach is presented and fragility analysis is performed with implementation of a new formulation in the component fragility analysis for MSSS bridge class and the outcome is compared with the one from conventional procedure. The results shows the correlation between demand and capacity affects the outcome of PBA and the fragility functions variation is not negligible. Therefore using the presented approach is necessary when accuracy is needed.
Show less - Date Issued
- 2014
- Identifier
- CFE0005531, ucf:50309
- Format
- Document (PDF)
- PURL
- http://purl.flvc.org/ucf/fd/CFE0005531
- Title
- Development of Traffic Safety Zones and Integrating Macroscopic and Microscopic Safety Data Analytics for Novel Hot Zone Identification.
- Creator
-
Lee, JaeYoung, Abdel-Aty, Mohamed, Radwan, Ahmed, Nam, Boo Hyun, Kuo, Pei-Fen, Choi, Keechoo, University of Central Florida
- Abstract / Description
-
Traffic safety has been considered one of the most important issues in the transportation field. With consistent efforts of transportation engineers, Federal, State and local government officials, both fatalities and fatality rates from road traffic crashes in the United States have steadily declined from 2006 to 2011.Nevertheless, fatalities from traffic crashes slightly increased in 2012 (NHTSA, 2013). We lost 33,561 lives from road traffic crashes in the year 2012, and the road traffic...
Show moreTraffic safety has been considered one of the most important issues in the transportation field. With consistent efforts of transportation engineers, Federal, State and local government officials, both fatalities and fatality rates from road traffic crashes in the United States have steadily declined from 2006 to 2011.Nevertheless, fatalities from traffic crashes slightly increased in 2012 (NHTSA, 2013). We lost 33,561 lives from road traffic crashes in the year 2012, and the road traffic crashes are still one of the leading causes of deaths, according to the Centers for Disease Control and Prevention (CDC). In recent years, efforts to incorporate traffic safety into transportation planning has been made, which is termed as transportation safety planning (TSP). The Safe, Affordable, Flexible Efficient, Transportation Equity Act (-) A Legacy for Users (SAFETEA-LU), which is compliant with the United States Code, compels the United States Department of Transportation to consider traffic safety in the long-term transportation planning process. Although considerable macro-level studies have been conducted to facilitate the implementation of TSP, still there are critical limitations in macroscopic safety studies are required to be investigated and remedied. First, TAZ (Traffic Analysis Zone), which is most widely used in travel demand forecasting, has crucial shortcomings for macro-level safety modeling. Moreover, macro-level safety models have accuracy problem. The low prediction power of the model may be caused by crashes that occur near the boundaries of zones, high-level aggregation, and neglecting spatial autocorrelation.In this dissertation, several methodologies are proposed to alleviate these limitations in the macro-level safety research. TSAZ (Traffic Safety Analysis Zone) is developed as a new zonal system for the macroscopic safety analysis and nested structured modeling method is suggested to improve the model performance. Also, a multivariate statistical modeling method for multiple crash types is proposed in this dissertation. Besides, a novel screening methodology for integrating two levels is suggested. The integrated screening method is suggested to overcome shortcomings of zonal-level screening, since the zonal-level screening cannot take specific sites with high risks into consideration. It is expected that the integrated screening approach can provide a comprehensive perspective by balancing two aspects: macroscopic and microscopic approaches.
Show less - Date Issued
- 2014
- Identifier
- CFE0005195, ucf:50653
- Format
- Document (PDF)
- PURL
- http://purl.flvc.org/ucf/fd/CFE0005195
